Recombinant Human/Mouse/Rat GDF-11/BMP-11 Protein, CF

Images

 
1 μg/lane of Recombinant Human/Mouse/Rat GDF‑11/BMP-11 was resolved withSDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by silver staining, showing bands at 13 kDa and 21 kDa, respectively.
Recombinant Human/Mouse/RatGDF‑11/BMP‑11 (Catalog # 1958-GD/CF) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 0.8-4.8 ng/mL.
ESI analysis of Recombinant Human/Mouse/Rat GDF-11/BMP-11. The peak at 25163 Da corresponds to the calculated molecular mass of the disulfide-linked homodimer.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Catalog# & Formulation Size Price

Recombinant Human/Mouse/Rat GDF-11/BMP-11 Protein, CF Summary

Details of Functionality
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.8-4.8 ng/mL.
Source
E. coli-derived GDF-11/BMP-11 protein
Asn299-Ser407, with an N-terminal Met
Accession #
N-terminal Sequence
Met-Asn299-Leu-Gly-Leu-Asp-(Cys)-Asp-Glu-His
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
GDF11
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1958-GD/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat GDF-11/BMP-11 Protein, CF

  • BMP-11
  • BMP-11BMP11Bone morphogenetic protein 11
  • GDF11
  • GDF-11
  • growth differentiation factor 11
  • growth/differentiation factor 11

Background

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide which contains a signal sequence for secretion and an RXXR proteolytic processing site to yield a 109 aa residue carboxy-terminal mature protein (1). Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99-100% identical. GDF-11 and GDF-8 share 90% amino acid sequence identity within the mature protein. As detected by in situ hybridization, GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1, 2). Targeted deletion of GDF-11, in mice, results in a spectrum of abnormalities including palatal malformation, vertebral defects, elongated trunks with a reduced or absent tail, missing or malformed kidneys, and an increased number of neurons in the olfactory epithelium (2-5). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (6). Systemic GDF-11 levels decline with age and administration of higher levels of GDF-11 can reverse age-related cardiac hypertrophy (7). In addition, systemic administration of recombinant GDF-11 protein restores genomic integrity and health of muscle stem cells, neurovasculature and enhances neurogenesis (8, 9). R&D Systems recombinant GDF-11 preparations have been shown to act similarly to GDF-8 in both the Xenopus animal cap and the K562 assays.
  1. Gamer, L.W. et al. (1999) Dev. Biol. 208: 222. 
  2. Nakashima, M. et al. (1999) Mech. Dev. 80:185.  
  3. Gad, J.M. and P.P.L. Tam (1999) Curr. Biol. 9:R783.  
  4. McPherron, A.C. et al. (1999) Nat. Genet. 22:260.
  5. Esquela, A.F. and S.J. Lee (2003) Dev. Biol. 257:356.
  6. Oh, S.P. et al. (2002) Genes & Dev. 16:274.
  7. Loffredo, F.S. et. al. (2013) Cell. 153.828.
  8. Katsimpardi, L. et. al. (2014) Science (ahead of print).
  9. Sinha, M. et. al. (2014) Science (ahead of print).

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1958-GD/CF
Species: Hu, Mu, Rt
Applications: Bioactivity

Publications for GDF-11/BMP-11 (1958-GD/CF)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.

We have publications tested in 4 applications: Bioassay, Immunocapture LCMS Standard, Surface Plasmon Resonance, WB - Ctrl.


Filter By Application
Bioassay
(10)
Immunocapture LCMS Standard
(1)
Surface Plasmon Resonance
(2)
WB - Ctrl
(1)
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Filter By Species
Human
(8)
Mouse
(4)
Rat
(1)
Xenopus
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 1958-GD/CF Applications Species
K Sakamoto, Y Kanematsu-, Y Kamada, M Oka, T Ohnishi, M Miwa, T Asami, H Inooka Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology Biochem Biophys Rep, 2017;11(0):33-39. 2017 [PMID: 28955765] (Bioassay, Human) Bioassay Human
E De Domenic, G D'Arcangel, I Faraoni, M Palmieri, V Tancredi, G Graziani, P Grimaldi, L Tentori Modulation of GDF11 expression and synaptic plasticity by age and training Oncotarget, 2017;8(35):57991-58002. 2017 [PMID: 28938532] (WB - Ctrl, Mouse) WB - Ctrl Mouse
JL Chen, KL Walton, A Hagg, TD Colgan, K Johnson, H Qian, P Gregorevic, CA Harrison Specific targeting of TGF-? family ligands demonstrates distinct roles in the regulation of muscle mass in health and disease Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28607086] (Bioassay, Human) Bioassay Human
Y Zhao, G Liu, FC Zambito, YJ Zhang, BS DeSilva, AT Kozhich, JX Shen A multiplexed immunocapture liquid chromatography tandem mass spectrometry assay for the simultaneous measurement of myostatin and GDF-11 in rat serum using an automated sample preparation platform Anal. Chim. Acta, 2017;979(0):36-44. 2017 [PMID: 28599707] (Immunocapture LCMS Standard, Rat) Immunocapture LCMS Standard Rat
YY Jing, D Li, F Wu, LL Gong, R Li GDF11 does not improve the palmitate induced insulin resistance in C2C12 Eur Rev Med Pharmacol Sci, 2017;21(8):1795-1802. 2017 [PMID: 28485800] (Bioassay, Mouse) Bioassay Mouse
E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada Activin A more prominently regulates muscle mass in primates than does GDF8 Nat Commun, 2017;8(0):15153. 2017 [PMID: 28452368] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
MM Mata-Miran, GJ Vazquez-Za, M Rojas-Lope, V Sanchez-Mo, DG Perez-Ishi, RJ Delgado-Ma Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells Biol. Res., 2017;50(1):14. 2017 [PMID: 28376862] (Bioassay, Mouse) Bioassay Mouse
SB Lee, SK Park, YS Kim Maltose binding protein-fusion enhances the bioactivity of truncated forms of pig myostatin propeptide produced in E. coli PLoS ONE, 2017;12(4):e0174956. 2017 [PMID: 28369115] (Bioassay, Human) Bioassay Human
DW Hammers, M Merscham-B, JY Hsiao, S Engst, JJ Hartman, HL Sweeney Supraphysiological levels of GDF11 induce striated muscle atrophy EMBO Mol Med, 2017;0(0):. 2017 [PMID: 28270449] (Bioassay, Human) Bioassay Human
RG Walker, M Czepnik, EJ Goebel, JC McCoy, A Vujic, M Cho, J Oh, S Aykul, KL Walton, G Schang, DJ Bernard, AP Hinck, CA Harrison, E Martinez-H, AJ Wagers, RT Lee, TB Thompson Structural basis for potency differences between GDF8 and GDF11 BMC Biol, 2017;15(1):19. 2017 [PMID: 28257634] (Bioassay, Human) Bioassay Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol GDF11
Uniprot