Recombinant Human MD-2 Protein, CF

Images

 
There are currently no images for MD-2 (1787-MD/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human MD-2 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When recombinant human TLR-4 is Immobilized at 2 µg/mL (100 µL/well), the concentration of rhMD-2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 µg/mL.
Source
E. coli-derived human MD2 protein
Met Human MD-2
(Glu17 - Asn160)
Accession # BAA78717
IEGRGGGSGGGSGGGS 10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
LY96
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
19.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1787-MD/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MD-2 Protein, CF

  • ESOP1
  • ESOP-1
  • LY96
  • ly-96
  • lymphocyte antigen 96
  • MD2
  • MD-2
  • myeloid differentiation protein-2
  • Protein MD-2

Background

MD-2, also known as lymphocyte antigen 96 and ESOP-1, is a secreted glycoprotein that shares conserved cysteine residues and significant sequence similarity (23%) with MD-1. The gene of human MD-2 encodes a 160 amino acid residue (aa) precursor protein with a 16 aa signal peptide and a 144 aa mature protein, which contains 2 N‑glycosylation sites (1). Recombinant secreted MD‑2 has been found to exist as disulfide-linked dimers and oligomers (2).

Both MD-1 and MD-2 are accessory molecules that associate with the extracellular leucine-rich repeats (LRR) of Toll-like receptor (TLR) family members, which are type I transmembrane receptors that regulate innate immune responses to microbial pathogens (3, 4). MD-1 binds to RP105 on B cells and macrophages to form the signaling receptor complex for lipopolysaccharide (LPS), a constituent of the outer membrane of Gram-negative bacteria. Similarly, MD-2 interacts with TLR-4 to form the heteromeric receptor that confers LPS responsiveness. MD-2 also associates with TLR-2, albeit with less avidity, to confer responsiveness to cell wall components from both Gram-positive and Gram-negative bacteria. MD-1 and MD-2 are also required for the correct targeting of the TLRs to the cell surface. Although MD-2 glycosylation is not crucial for its surface expression and interaction with TLR-4, it is required for LPS binding and signaling (5).

  1. Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
  2. Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12156.
  3. Nagai, Y. et al. (2002) Nature Immunology 3:667.
  4. Akashi, S. et al. (2003) J. Exp. Med. 198:1035.
  5. Correia, J. and R. Ulevitch (2002) J. Biol. Chem. 277:1845.

Customers Who Viewed This Item Also Viewed...

NB100-56566
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
DC140
Species: Hu
Applications: ELISA
NBP2-93743
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NB100-56722
Species: Ca, Hu, Mu, Rb, Rt
Applications: B/N, DB, ELISA, Flow-CS, Flow, Func, ICC/IF, IP, In vitro, WB
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
DY870-05
Species: Hu
Applications: ELISA
D6050
Species: Hu
Applications: ELISA
NBP2-29328
Species: Bv, Hu, Mu, Rt, Xp, Ye, Ze
Applications: BindInhib, B/N, ELISA, Flow, Func-Inh, IHC, In vitro, In vivo
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
NB100-56700
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-79854
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, PA, WB
NBP1-84915
Species: Hu
Applications: IHC, IHC-P, WB
NB100-56563
Species: Hu, Mu, Rt
Applications: DB, Flow-CS, Flow-IC, Flow, IHC, IHC-P, WB
NBP2-20204
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB120-13810
Species: Mu, Po, Rt
Applications: IB, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB
DY417
Species: Mu
Applications: ELISA

Publications for MD-2 (1787-MD/CF)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Bacteria - Taiwanofungus camphoratus, N/A.

We have publications tested in 6 applications: Bioassay, ELISA (Standard), ELISA Capture, ELISA Developmet, In Vivo, SPR.


Filter By Application
Bioassay
(10)
ELISA (Standard)
(1)
ELISA Capture
(1)
ELISA Developmet
(1)
In Vivo
(1)
SPR
(1)
All Applications
Filter By Species
Human
(9)
Mouse
(1)
Bacteria - Taiwanofungus camphoratus
(1)
N/A
(2)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 1787-MD/CF Applications Species
W Zhu, NE Sahar, HMA Javaid, ES Pak, G Liang, Y Wang, H Ha, JY Huh Exercise-Induced Irisin Decreases Inflammation and Improves NAFLD by Competitive Binding with MD2 Cells, 2021;10(12):. 2021 [PMID: 34943814] (Bioassay, Mouse) Bioassay Mouse
W Luo, LB Yang, CC Qian, B Ma, GM Manjengwa, XM Miao, J Wang, CH Hu, B Jin, LX Zhang, C Zheng, G Liang, Y Wang Flavokawain B alleviates LPS-induced acute lung injury via targeting myeloid differentiation factor 2 Acta pharmacologica Sinica, 2021;0(0):. 2021 [PMID: 34737421] (SPR, Human) SPR Human
A Puck, S Künig, M Modak, L May, P Fritz, C Battin, K Radakovics, P Steinberge, BM Reipert, BA Crowe, J Stöckl The soluble cytoplasmic tail of CD45 regulates T cell activation via TLR4 signaling European Journal of Immunology, 2021;0(0):. 2021 [PMID: 34626426] (Bioassay, Human) Bioassay Human
S Sun, Z Yan, X Shui, W Qi, Y Chen, X Xu, Y Hu, W Guo, P Shang Astilbin prevents osteoarthritis development through the TLR4/MD-2 pathway J Cell Mol Med, 2020;0(0):. 2020 [PMID: 33063931] (ELISA Capture, Human) ELISA Capture Human
Y Wang, W Luo, J Han, ZA Khan, Q Fang, Y Jin, X Chen, Y Zhang, M Wang, J Qian, W Huang, H Lum, G Wu, G Liang MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy Nat Commun, 2020;11(1):2148. 2020 [PMID: 32358497] (Bioassay, Human) Bioassay Human
T Chen, W Huang, J Qian, W Luo, P Shan, Y Cai, K Lin, G Wu, G Liang Macrophage-derived myeloid differentiation protein 2 plays an essential role in ox-LDL-induced inflammation and atherosclerosis EBioMedicine, 2020;53(0):102706. 2020 [PMID: 32151799] (Bioassay, Human) Bioassay Human
P Shang, Q Tang, Z Hu, S Huang, Y Hu, J Zhu, H Liu Procyanidin B3 alleviates intervertebral disc degeneration via interaction with the TLR4/MD-2 complex J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32068951] (ELISA (Standard)) ELISA (Standard)
T Ito, S Yamamoto, K Yamaguchi, M Sato, Y Kaneko, S Goto, Y Goto, I Narita Inorganic polyphosphate potentiates lipopolysaccharide-induced macrophage inflammatory response J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32041779] (Bioassay, N/A) Bioassay N/A
S Sun, M He, Y Wang, H Yang, Y Al-Abed Folic acid derived-P5779 mimetics regulate DAMP-mediated inflammation through disruption of HMGB1:TLR4:MD-2 axes PLoS ONE, 2018;13(2):e0193028. 2018 [PMID: 29447234] (Bioassay) Bioassay
B S Lashkari, DO Anumba Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4 PLoS ONE, 2017;12(3):e0173646. 2017 [PMID: 28296959] (Bioassay, Human) Bioassay Human
Show All 15 Publications.

Reviews for MD-2 (1787-MD/CF) (0)

There are no reviews for MD-2 (1787-MD/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for MD-2 (1787-MD/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human MD-2 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol LY96
Uniprot