Recombinant Human Lipocalin-2/NGAL Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human Lipocalin-2/NGAL Protein, CF Summary

Details of Functionality
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. rhLipocalin-2 can bind >1.5 µM of Fe(DHBA)3 under the described conditions.
Mouse myeloma cell line, NS0-derived human Lipocalin-2/NGAL protein
Gln21-Gly198, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
No results obtained: Gln21 predicted
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
25-27 kDa doublet, reducing conditions
Read Publications using
1757-LC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in MES and NaCl.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (TCN)
  • Recombinant Human Lipocalin‑2/NGAL (rhLipocalin-2) (Catalog # 1757-LC)
  • Iron III (Fe3+) (Sigma, Catalog # 16596)
  • 2,3,Dihydroxybenzoic Acid (DHBA) (Sigma, Catalog # 126209)
  • Ligand Buffer: 0.1 M Tris, pH 8.0
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
    1. Prepare a curve of Fe3+ in deionized water with the following serial dilutions: 640, 320, 160, 80, 40, 20, 10, 5, and 2.5 µM.
    2. Prepare 1 mM DHBA in Ligand Buffer from powder stock.
    3. Combine equal volumes of the Fe3+ curve with 1 mM DHBA. Include a control containing 1 mM DHBA and Ligand Buffer.
    4. Incubate at room temperature for 10 minutes. A curve of the metal ligand complex of Fe(DHBA)3 is formed.
    5. After incubation, perform 5 fold dilutions to the curve using Assay Buffer.
    6. Dilute rhLipocalin-2 (MW: 21905 Da) to 4 µM in Assay Buffer.
    7. In the plate, load 50 µL of the diluted Fe(DHBA)3 complex curve and 50 µL of 4 µM rhLipocalin-2.
    8. Incubate at room temperature for 30 minutes.
    9. Read at excitation and emission wavelengths of 280 nM and 340 nM, respectively in endpoint mode.
    10. Plot a 4-parameter curve of Fe(DHBA)3 concentration (x-axis) versus RFUs (y-axis), and calculate a BC50 from the curve.
Per Well:
  • Fe(DHBA)3 Curve: 0.001, 0.125, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16, and 32 µM.
  • rhLipocalin-2: 2 µM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Lipocalin-2/NGAL Protein, CF

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin


Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

  1. Flower, D.R. et al. (1994) FEBS Lett. 354:7.
  2. Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426.
  3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  4. Devireddy, L.R. et al. (2001) Science 293: 829.
  5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  7. Flo, T.H. et al. (2004) Nature 432:917.
  8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

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Publications for Lipocalin-2/NGAL (1757-LC)(23)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Salmonella.

We have publications tested in 5 applications: Bioassay, Cell Culture, Enzyme Assay, Stimulation, Western Blot.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using 1757-LC Applications Species
W Santus, AP Rana, JR Devlin, KA Kiernan, CC Jacob, J Tjokrosurj, DM Underhill, J Behnsen Mycobiota and diet-derived fungal xenosiderophores promote Salmonella gastrointestinal colonization Nature Microbiology, 2022;7(12):2025-2038. 2022 [PMID: 36411353] (Bioassay, Salmonella) Bioassay Salmonella
U Gupta, S Ghosh, CT Wallace, P Shang, Y Xin, AP Nair, M Yazdankhah, A Strizhakov, MA Ross, H Liu, S Hose, NA Stepicheva, O Chowdhury, M Nemani, V Maddipatla, R Grebe, M Das, KL Lathrop, JA Sahel, JS Zigler, J Qian, A Ghosh, Y Sergeev, JT Handa, CM St Croix, D Sinha Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD Autophagy, 2022;0(0):1-20. 2022 [PMID: 35473441] (Bioassay, Human) Bioassay Human
L Cook, M Sengelmann, B Winkler, C Nagl, S Koch, U Schlomann, EP Slater, MA Miller, EP von Strand, B Dörsam, C Preu beta er, JW Bartsch ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9 International Journal of Molecular Sciences, 2022;23(4):. 2022 [PMID: 35216088] (Stimulation, Human) Stimulation Human
SK Schröder, M Pinoé-Schm, R Weiskirche Lipocalin-2 (LCN2) Deficiency Leads to Cellular Changes in Highly Metastatic Human Prostate Cancer Cell Line PC-3 Cells, 2022;11(2):. 2022 [PMID: 35053376] (Bioassay, Human) Bioassay Human
W Zhang, R Pan, M Lu, Q Zhang, Z Lin, Y Qin, Z Wang, S Gong, H Lin, S Chong, L Lu, W Liao, X Lu Epigenetic induction of lipocalin 2 expression drives acquired resistance to 5-fluorouracil in colorectal cancer through integrin beta3/SRC pathway Oncogene, 2021;0(0):. 2021 [PMID: 34588619] (Bioassay, Human) Bioassay Human
X Wen, B Su, M Gao, J Chen, D Zhou, H You, N Li, S Chang, X Cheng, C Qian, J Gao, P Yang, S Qu, L Bu Obesity-associated up-regulation of lipocalin 2 protects gastric mucosa cells from apoptotic cell death by reducing endoplasmic reticulum stress Cell Death & Disease, 2021;12(2):221. 2021 [PMID: 33637683] (Bioassay, Human) Bioassay Human
A Tyagi, S Sharma, K Wu, SY Wu, F Xing, Y Liu, D Zhao, RP Deshpande, RB D'Agostino, K Watabe Nicotine promotes breast cancer metastasis by stimulating N2 neutrophils and generating pre-metastatic niche in lung Nature Communications, 2021;12(1):474. 2021 [PMID: 33473115] (Bioassay, Human) Bioassay Human
Z Hui, Z Jiang, D Qiao, Z Bo, K Qiyuan, B Shaohua, Y Wenbing, L Wei, L Cheng, L Shuangning, L Zhengyi, L Yingyi Increased expression of LCN2 formed a positive feedback loop with activation of the ERK pathway in human kidney cells during kidney stone formation Scientific Reports, 2020;10(1):21287. 2020 [PMID: 33277533] (Cell Culture, Human) Cell Culture Human
K Yahiro, K Ogura, Y Goto, S Iyoda, T Kobayashi, H Takeuchi, M Ohnishi, J Moss Subtilase cytotoxin induces a novel form of Lipocalin 2, which promotes Shiga-toxigenic Escherichia coli survival Sci Rep, 2020;10(1):18943. 2020 [PMID: 33144618] (Cell Culture, Human) Cell Culture Human
B Bauvois, E Pramil, L Jondrevill, E Chapiro, C Quiney, K Maloum, SA Susin, F Nguyen-Kha Relation of Neutrophil Gelatinase-Associated Lipocalin Overexpression to the Resistance to Apoptosis of Tumor B Cells in Chronic Lymphocytic Leukemia Cancers (Basel), 2020;12(8):. 2020 [PMID: 32751884] (Bioassay, Human) Bioassay Human
Show All 23 Publications.

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Gene Symbol LCN2