Recombinant Human Leptin Protein, CF

Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human Leptin R. The ED50 for this effect is 0.4-2 ng/mL.

1 μg/lane of Recombinant Human Leptin/OB was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Leptin Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human Leptin R. The ED 50 for this effect is 0.4-2 ng/mL.
• Source: E. coli-derived human Leptin/OB protein Val22-Cys167, with an N-terminal Met
• Accession #: Q6NT58
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: LEP
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 3 months, 2 to 8 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCl, pH 8.0.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Leptin Protein, CF

• FLJ94114
• LEP
• leptin (murine obesity homolog)
• leptin (obesity homolog, mouse)
• Leptin
• OB
• Obese protein
• obese, mouse, homolog of
• Obesity factor
• OBOBS

Background

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.

Publications for Leptin/OB (398-LP)(44)

Publications using 398-LP

• Giuranna, J;Zheng, Y;Brandt, M;Jall, S;Mukherjee, A;Shankhwar, S;Renner, S;Kurapati, NK;May, C;Peters, T;Herpertz-Dahlmann, B;Seitz, J;de Zwaan, M;Herzog, W;Ehrlich, S;Zipfel, S;Giel, K;Egberts, K;Burghardt, R;Föcker, M;Marcus, K;Keyvani, K;Müller, TD;Schmitz, F;Rajcsanyi, LS;Hinney, A; Genetic and functional analyses of CTBP2 in anorexia nervosa and body weight regulation Molecular psychiatry 2024-11-07 [PMID: 39511451] (In vivo assay, Mouse)
• Fontes-Villalba, M;Granfeldt, Y;Sundquist, K;Memon, AA;Hedelius, A;Carrera-Bastos, P;Jönsson, T; Effects of a Paleolithic diet compared to a diabetes diet on leptin binding inhibition in secondary analysis of a randomised cross-over study BMC endocrine disorders 2024-09-04 [PMID: 39232748] (Bioassay, N/A)
• Akindehin, S;Liskiewicz, A;Liskiewicz, D;Bernecker, M;Garcia-Caceres, C;Drucker, DJ;Finan, B;Grandl, G;Gutgesell, R;Hofmann, SM;Khalil, A;Liu, X;Cota, P;Bakhti, M;Czarnecki, O;Bastidas-Ponce, A;Lickert, H;Kang, L;Maity, G;Novikoff, A;Parlee, S;Pathak, E;Schriever, SC;Sterr, M;Ussar, S;Zhang, Q;DiMarchi, R;Tschöp, MH;Pfluger, PT;Douros, JD;Müller, TD; Loss of GIPR in LEPR cells impairs glucose control by GIP and GIP:GLP-1 co-agonism without affecting body weight and food intake in mice Molecular metabolism 2024-03-14 [PMID: 38492844] (In vivo assay, Mouse)
• Masloh, S;Chevrel, A;Culot, M;Perrocheau, A;Kalia, YN;Frehel, S;Gaussin, R;Gosselet, F;Huet, S;Zeisser Labouebe, M;Scapozza, L; Enhancing Oral Delivery of Biologics: A Non-Competitive and Cross-Reactive Anti-Leptin Receptor Nanofitin Demonstrates a Gut-Crossing Capacity in an Ex Vivo Porcine Intestinal Model Pharmaceutics 2024-01-16 [PMID: 38258126] (Bioassay, Human)
• Pries, R;Kosyna, FK;Depping, R;Plötze-Martin, K;Lange, C;Meyhöfer, S;Meyhöfer, SM;Marquardt, JU;Bruchhage, KL;Steffen, A; Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets Scientific reports 2024-01-03 [PMID: 38172514] (Bioassay, Human)
• Flores Gomez, D;Bekkering, S;Horst, RT;Cossins, B;van den Munckhof, ICL;Rutten, JHW;Joosten, LAB;Netea, MG;Riksen, NP; The effect of leptin on trained innate immunity and on systemic inflammation in subjects with obesity Journal of leukocyte biology 2023-09-30 [PMID: 37776323] (Bioassay, Human)
• Y Kayashima, WHD Townley-Ti, NL Vora, K Boggess, JW Homeister, N Maeda-Smit, F Li Insulin Elevates ID2 Expression in Trophoblasts and Aggravates Preeclampsia in Obese ASB4-Null Mice International Journal of Molecular Sciences, 2023-01-21;24(3):. 2023-01-21 [PMID: 36768469] (Bioassay, Human)
• J Wei, X Huang, X Zhang, G Chen, C Zhang, X Zhou, J Qi, Y Zhang, X Li Elevated fatty acid beta-oxidation by leptin contributes to the proinflammatory characteristics of fibroblast-like synoviocytes from RA patients via LKB1-AMPK pathway Cell Death & Disease, 2023-02-09;14(2):97. 2023-02-09 [PMID: 36759597] (Bioassay, Human)
• K Watanabe, M Suzukawa, S Kawauchi-W, S Igarashi, I Asari, S Imoto, H Tashimo, T Fukami, A Hebisawa, S Tohma, T Nagase, K Ohta Leptin-producing monocytes in the airway submucosa may contribute to asthma pathogenesis Respiratory investigation, 2022-11-09;0(0):. 2022-11-09 [PMID: 36369154] (Western Blot Control, Human)
• E Mourmoura, I Papathanas, V Trachana, V Konteles, A Tsoumpou, A Goutas, AA Papageorgi, N Stefanou, A Tsezou Leptin-depended NLRP3 inflammasome activation in osteoarthritic chondrocytes is mediated by ROS Mechanisms of Ageing and Development, 2022-09-07;208(0):111730. 2022-09-07 [PMID: 36087742] (Bioassay, Human)
• X Wang, Y Shen, X Ke, H Kang, S Hong Role of leptin/ILC2 axis in allergic rhinitis in obese children International journal of pediatric otorhinolaryngology, 2022-04-19;157(0):111127. 2022-04-19 [PMID: 35468486] (Bioassay, Human)
• M Gao, M Ge, J Huo, X Ren, X Li, Y Shao, J Huang, J Zhang, M Wang, N Nie, P Jin, Y Zheng Leptin-mediated proinflammatory bone marrow environment in acquired aplastic anemia Cytokine, 2022-02-23;152(0):155829. 2022-02-23 [PMID: 35217430] (Bioassay, Human)
• I Thomas, I Panagoulia, I Aggeletopo, A Varvarigou, BE Spiliotis, A Mouzaki The Role of Leptin in Childhood Immune Thrombocytopenia (ITP): An Anti-Inflammatory Agent? International Journal of Molecular Sciences, 2021-07-16;22(14):. 2021-07-16 [PMID: 34299256] (Bioassay, Human)
• B Leibiger, T Moede, I Valladolid, M Paschen, M Visa, IB Leibiger, PO Berggren Ectopic Leptin Production by Intraocular Pancreatic Islet Organoids Ameliorates the Metabolic Phenotype of ob/ob Mice Metabolites, 2021-06-14;11(6):. 2021-06-14 [PMID: 34198579] (In Vivo, Mouse)
• DR Crabtree, W Buosi, CL Fyfe, GW Horgan, Y Manios, O Androutsos, A Giannopoul, G Finlayson, K Beaulieu, CL Meek, JJ Holst, KV Van Norren, JG Mercer, AM Johnstone Appetite Control across the Lifecourse: The Acute Impact of Breakfast Drink Quantity and Protein Content. The Full4Health Project Nutrients, 2020-11-30;12(12):. 2020-11-30 [PMID: 33266325] (ELISA (Standard), Human)
• SN Ewbank, CA Campos, JY Chen, AJ Bowen, SL Padilla, JL Dempsey, JY Cui, RD Palmiter Chronic Gq signaling in AgRP neurons does not cause obesity Proc. Natl. Acad. Sci. U.S.A., 2020-08-06;0(0):. 2020-08-06 [PMID: 32764144] (In Vivo, Mouse)
• F Gu, H Zhang, L Yao, S Jiang, H Lu, X Xing, C Zhang, P Jiang, R Zhang Leptin contributes to the taxol chemoresistance in epithelial ovarian cancer Oncol Lett, 2019-05-21;18(1):561-570. 2019-05-21 [PMID: 31289528] (Bioassay, Human)
• K Watanabe, M Suzukawa, S Arakawa, K Kobayashi, S Igarashi, H Tashimo, H Nagai, S Tohma, T Nagase, K Ohta Leptin enhances cytokine/chemokine production by normal lung fibroblasts by binding to leptin receptor Allergol Int, 2019-04-24;0(0):. 2019-04-24 [PMID: 31029506] (Bioassay, Human)
• F Yang, R Wu, Z Jiang, J Chen, J Nan, S Su, N Zhang, C Wang, J Zhao, C Ni, Y Wang, W Hu, Z Zeng, K Zhu, X Liu, X Hu, W Zhu, H Yu, J Huang, J Wang Leptin increases mitochondrial OPA1 via GSK3-mediated OMA1 ubiquitination to enhance therapeutic effects of mesenchymal stem cell transplantation Cell Death Dis, 2018-05-01;9(5):556. 2018-05-01 [PMID: 29748581] (Bioassay, Human)
• J Oswald, M Büttner, S Jasinsky-B, R Jacobs, P Rosenstock, H Kielstein Leptin affects filopodia and cofilin in NK-92 cells in a dose- and time-dependent manner Eur J Histochem, 2018-01-26;62(1):2848. 2018-01-26 [PMID: 29569869] (Bioassay, Human)
• F Riu, SC Slater, EJ Garcia, I Rodriguez-, V Alvino, E Avolio, G Mangialard, A Cordaro, S Satchell, C Zebele, A Caporali, G Angelini, P Madeddu The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling Sci Rep, 2017-07-14;7(1):5443. 2017-07-14 [PMID: 28710369] (Bioassay, Human)
• D Daley-Brow, G Oprea-Iles, KT Vann, V Lanier, R Lee, PV Candelaria, A Quarshie, R Pattillo, RR Gonzalez-P Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation Dis. Markers, 2017-06-04;2017(0):8248175. 2017-06-04 [PMID: 28659656] (Bioassay, Human)
• A Harbuzariu, A Rampoldi, DS Daley-Brow, P Candelaria, TL Harmon, CC Lipsey, DJ Beech, A Quarshie, GO Ilies, RR Gonzalez-P Leptin-Notch signaling axis is involved in pancreatic cancer progression Oncotarget, 2017-01-31;8(5):7740-7752. 2017-01-31 [PMID: 27999190] (Bioassay, Human)
• Robert A Hart Leptin pharmacokinetics in male mice Endocr Connect, 2016-12-20;0(0):. 2016-12-20 [PMID: 27998953] (In Vivo, Mouse)
• Chang C, Wu M, Yang J, Camarillo I, Chang C Leptin-STAT3-G9a Signaling Promotes Obesity-Mediated Breast Cancer Progression. Cancer Res, 2015-04-03;75(11):2375-86. 2015-04-03 [PMID: 25840984] (Bioassay, Human)
• Tadokoro , Susumu, Ide , Shinji, Tokuyama , Reiko, Umeki , Hirochik, Tatehara , Seiko, Kataoka , Shiki, Satomura , Kazuhito Leptin promotes wound healing in the skin. PLoS ONE, 2015-03-23;10(3):e0121242. 2015-03-23 [PMID: 25799398] (Bioassay, Human)
• Liu , Tao, Zhang , Yong, Liu , Yan, Wang , Ying, Jia , Haiqun, Kang , Mingchao, Luo , Xiaozhou, Caballero , Dawna, Gonzalez , Jose, Sherwood , Lance, Nunez , Vanessa, Wang , Danling, Woods , Ashley, Schultz , Peter G, Wang , Feng Functional human antibody CDR fusions as long-acting therapeutic endocrine agonists. Proc Natl Acad Sci U S A, 2015-01-20;112(5):1356-61. 2015-01-20 [PMID: 25605877] (Bioassay, Mouse)
• Huang , Guian, Li , Juan, Wang , Hongning, Lan , Xinyu, Wang , Yajun Discovery of a novel functional leptin protein (LEP) in zebra finches: evidence for the existence of an authentic avian leptin gene predominantly expressed in the brain and pituitary. Endocrinology, 2014-05-13;155(9):3385-96. 2014-05-13 [PMID: 24823393] (Bioassay, Human)
• Dong , Z, Fu , S, Xu , X, Yang , Y, Du , L, Li , W, Kan , S, Li , Z, Zhang , X, Wang , L, Li , J, Liu , H, Qu , X, Wang , C Leptin-mediated regulation of ICAM-1 is Rho/ROCK dependent and enhances gastric cancer cell migration. Br J Cancer, 2014-02-18;110(7):1801-10. 2014-02-18 [PMID: 24548863] (Bioassay, Human)
• Libraty D, Zhang L, Woda M, Giaya K, Kathivu C, Acosta L, Tallo V, Segubre-Mercado E, Bautista A, Obcena A, Brion J, Capeding R Low adiposity during early infancy is associated with a low risk for developing dengue hemorrhagic fever: a preliminary model. PLoS ONE, 2014-02-12;9(2):e88944. 2014-02-12 [PMID: 24533162] (Bioassay, Human)
• Lo H, Lai T, Li C, Wu W TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways. Acta Pharmacol Sin, 2014-02-03;35(3):339-50. 2014-02-03 [PMID: 24487964] (Bioassay, Human)
• Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1. Cytokine, 2013-11-23;65(2):222-30. 2013-11-23 [PMID: 24275551] (Bioassay, Human)
• Schroeter M, Eschholz N, Herzberg S, Jerchel I, Leifheit-Nestler M, Czepluch F, Chalikias G, Konstantinides S, Schafer K Leptin-dependent and leptin-independent paracrine effects of perivascular adipose tissue on neointima formation. Arterioscler Thromb Vasc Biol, 2013-03-21;33(5):980-7. 2013-03-21 [PMID: 23520165] (Bioassay, Human)
• Jaedicke K, Roythorne A, Padget K, Todryk S, Preshaw P, Taylor J Leptin up-regulates TLR2 in human monocytes. J Leukoc Biol, 2013-01-22;93(4):561-71. 2013-01-22 [PMID: 23341537] (Bioassay, Human)
• Lam KK, Chiu PC, Lee CL Glycodelin-A protein interacts with Siglec-6 protein to suppress trophoblast invasiveness by down-regulating extracellular signal-regulated kinase (ERK)/c-Jun signaling pathway. J. Biol. Chem., 2011-08-31;286(43):37118-27. 2011-08-31 [PMID: 21880722] (Binding Assay, Human)
• Suzukawa M, Nagase H, Ogahara I, Han K, Tashimo H, Shibui A, Koketsu R, Nakae S, Yamaguchi M, Ohta K Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils. J. Immunol., 2011-03-18;186(9):5254-60. 2011-03-18 [PMID: 21421855] (Bioassay, Human)
• Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-06-08;56(0):1320. 2010-06-08 [PMID: 20530731] (ELISA (Standard))
• Procopio C, Andreozzi F, Laratta E, Cassese A, Beguinot F, Arturi F, Hribal ML, Perticone F, Sesti G Leptin-stimulated endothelial nitric-oxide synthase via an adenosine 5'-monophosphate-activated protein kinase/Akt signaling pathway is attenuated by interaction with C-reactive protein. Endocrinology, 2009-04-09;150(8):3584-93. 2009-04-09 [PMID: 19359389] (In Vivo, Mouse)
• Dellas C, Schafer K, Rohm I, Lankeit M, Ellrott T, Faustin V, Riggert J, Hasenfuss G, Konstantinides S Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity. Thromb. Haemost., 2008-12-01;100(6):1123-9. 2008-12-01 [PMID: 19132239] (Bioassay, Human)
• Emsley HC, Smith CJ, Gavin CM Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production. BMC Neurol, 2007-02-28;7(0):5. 2007-02-28 [PMID: 17328808] (ELISA (Standard))
• Durakoglugil M, Irving AJ, Harvey J Leptin induces a novel form of NMDA receptor-dependent long-term depression. J. Neurochem., 2005-08-08;95(2):396-405. 2005-08-08 [PMID: 16086687] (In Vivo, Rat)
• Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis. J. Assist. Reprod. Genet., 2005-06-01;22(6):257-64. 2005-06-01 [PMID: 16021855] (ELISA (Standard))
• Mistrik P, Moreau F, Allen JM BiaCore analysis of leptin-leptin receptor interaction: evidence for 1:1 stoichiometry. Anal. Biochem., 2004-04-15;327(2):271-7. 2004-04-15 [PMID: 15051545] (Surface Plasmon Resonance, Human, Mouse)
• Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes. Eur. Cytokine Netw., 2002-04-01;13(2):225-9. 2002-04-01 [PMID: 12101078] (ELISA (Standard))

Bioinformatics

• Gene Symbol: LEP
• Uniprot:
  • Q6NT58()