Recombinant Human Leptin Protein, CF

Images

 
1 μg/lane of Recombinant Human Leptin/OB was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human Leptin R. The ED50 for this effect is 0.4-2 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human Leptin Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human Leptin R. The ED50 for this effect is 0.4‑2 ng/mL.
Source
E. coli-derived
Val22-Cys167, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
LEP
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
398-LP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCl, pH 8.0.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Leptin Protein, CF

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Background

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

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Publications for (23)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 4 applications: Bioassay, Competitive Binding Assay, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(15)
Competitive Binding Assay
(1)
ELISA Standard
(3)
In Vivo
(2)
All Applications
Filter By Species
Human
(15)
Mouse
(2)
Rat
(1)
N/A
(3)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using 398-LP Applications Species
F Riu, SC Slater, EJ Garcia, I Rodriguez-, V Alvino, E Avolio, G Mangialard, A Cordaro, S Satchell, C Zebele, A Caporali, G Angelini, P Madeddu The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling Sci Rep, 2017;7(1):5443. 2017 [PMID: 28710369] (Bioassay, Human) Bioassay Human
D Daley-Brow, G Oprea-Iles, KT Vann, V Lanier, R Lee, PV Candelaria, A Quarshie, R Pattillo, RR Gonzalez-P Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation Dis. Markers, 2017;2017(0):8248175. 2017 [PMID: 28659656] (Bioassay, Human) Bioassay Human
A Harbuzariu, A Rampoldi, DS Daley-Brow, P Candelaria, TL Harmon, CC Lipsey, DJ Beech, A Quarshie, GO Ilies, RR Gonzalez-P Leptin-Notch signaling axis is involved in pancreatic cancer progression Oncotarget, 2017;8(5):7740-7752. 2017 [PMID: 27999190] (Bioassay, Human) Bioassay Human
Chang C, Wu M, Yang J, Camarillo I, Chang C Leptin-STAT3-G9a Signaling Promotes Obesity-Mediated Breast Cancer Progression. Cancer Res, 2015;75(11):2375-86. 2015 [PMID: 25840984] (Bioassay, Human) Bioassay Human
Tadokoro, Susumu, Ide, Shinji, Tokuyama, Reiko, Umeki, Hirochik, Tatehara, Seiko, Kataoka, Shiki, Satomura, Kazuhito Leptin promotes wound healing in the skin. PLoS ONE, 2015;10(3):e0121242. 2015 [PMID: 25799398] (Bioassay, Human) Bioassay Human
Liu, Tao, Zhang, Yong, Liu, Yan, Wang, Ying, Jia, Haiqun, Kang, Mingchao, Luo, Xiaozhou, Caballero, Dawna, Gonzalez, Jose, Sherwood, Lance, Nunez, Vanessa, Wang, Danling, Woods, Ashley, Schultz, Peter G, Wang, Feng Functional human antibody CDR fusions as long-acting therapeutic endocrine agonists. Proc Natl Acad Sci U S A, 2015;112(5):1356-61. 2015 [PMID: 25605877] (Bioassay, Mouse) Bioassay Mouse
Huang, Guian, Li, Juan, Wang, Hongning, Lan, Xinyu, Wang, Yajun Discovery of a novel functional leptin protein (LEP) in zebra finches: evidence for the existence of an authentic avian leptin gene predominantly expressed in the brain and pituitary. Endocrinology, 2014;155(9):3385-96. 2014 [PMID: 24823393] (Bioassay, Human) Bioassay Human
Dong, Z, Fu, S, Xu, X, Yang, Y, Du, L, Li, W, Kan, S, Li, Z, Zhang, X, Wang, L, Li, J, Liu, H, Qu, X, Wang, C Leptin-mediated regulation of ICAM-1 is Rho/ROCK dependent and enhances gastric cancer cell migration. Br J Cancer, 2014;110(7):1801-10. 2014 [PMID: 24548863] (Bioassay, Human) Bioassay Human
Libraty D, Zhang L, Woda M, Giaya K, Kathivu C, Acosta L, Tallo V, Segubre-Mercado E, Bautista A, Obcena A, Brion J, Capeding R Low adiposity during early infancy is associated with a low risk for developing dengue hemorrhagic fever: a preliminary model. PLoS ONE, 2014;9(2):e88944. 2014 [PMID: 24533162] (Bioassay, Human) Bioassay Human
Lo H, Lai T, Li C, Wu W TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways. Acta Pharmacol Sin, 2014;35(3):339-50. 2014 [PMID: 24487964] (Bioassay, Human) Bioassay Human
Show All 23 Publications.

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Bioinformatics

Gene Symbol LEP
Entrez
Uniprot