Recombinant Human IL-4 Protein, CF


1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-4 (Catalog # 204‑IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.05‑02 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications BA

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Recombinant Human IL-4 Protein, CF Summary

Additional Information
A New rhIL-4 is Now Available! The new protein is mammalian cell expressed!
New Product 6507-IL
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.
The specific activity of recombinant human IL-4 is approximately 2.9 x 104 IU/μg, which is calibrated against human IL-4 WHO International Standard (NIBSC code: 88/656).
E. coli-derived
His25-Ser153, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
14 kDa, reducing conditions
Read Publications using
204-IL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-4 Protein, CF

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra


Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

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Publications for (259)

We have publications tested in 7 confirmed species: Human, Mouse, Chinchilla, N/A, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, ELISA Standard, Ex Vivo, cell culture.

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Primate - Chlorocebus pygerythrus (Vervet Monkey)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 259. Show All 259 Publications.
Publications using 204-IL/CF Applications Species
J Zeitvogel, N Jokmin, S Rieker, I Klug, C Brandenber, T Werfel GATA3 regulates FLG and FLG2 expression in human primary keratinocytes Sci Rep, 2017;7(1):11847. 2017 [PMID: 28928464] (Bioassay, Human) Bioassay Human
M Yoshida, Y Ishioka, T Ozawa, H Okuyama, M Iguchi, T Ota, T Ito, M Nagira, A Morita, H Tanaka, H Naito, H Kidoya, N Takakura Soluble HLA-associated peptide from PSF1 has a cancer vaccine potency Sci Rep, 2017;7(1):11137. 2017 [PMID: 28894200] (Bioassay, Human) Bioassay Human
L Sarmiento, J De Rosa, A Machicote, LA Billordo, P Baz, PM Fernández, I Kaimen Mac, A Blanco, EI Arana Characterization of tonsillar IL10 secreting B cells and their role in the pathophysiology of tonsillar hypertrophy Sci Rep, 2017;7(1):11077. 2017 [PMID: 28894116] (Bioassay, Human) Bioassay Human
J Helft, F Anjos-Afon, AG van der Ve, P Chakravart, D Bonnet, C Reis E Sou Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors Cell Rep, 2017;20(3):529-537. 2017 [PMID: 28723558] (Bioassay, Human) Bioassay Human
DJ Son, YY Jung, MH Park, HL Lee, MJ Song, HS Yoo, DY Hwang, SB Han, JT Hong Activated Natural Killer Cells Mediate the Suppressive Effect of Interleukin-4 on Tumor Development via STAT6 Activation in an Atopic Condition Melanoma Model Neoplasia, 2017;19(7):537-548. 2017 [PMID: 28587956] (Bioassay, Human) Bioassay Human
AM Clemente, G Castronovo, A Antonelli, MM D'Andrea, M Tanturli, E Perissi, S Paccosi, A Parenti, F Cozzolino, GM Rossolini, MG Torcia Differential Th17 response induced by the two clades of the pandemic ST258 Klebsiella pneumoniae clonal lineages producing KPC-type carbapenemase PLoS ONE, 2017;12(6):e0178847. 2017 [PMID: 28586386] (Bioassay, Human) Bioassay Human
JY Ao, XD Zhu, ZT Chai, H Cai, YY Zhang, KZ Zhang, LQ Kong, N Zhang, BG Ye, DN Ma, HC Sun Colony-stimulating factor 1 receptor blockade inhibits tumor growth by altering the polarization of tumor-associated macrophages in hepatocellular carcinoma Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28572167] (Bioassay, Human) Bioassay Human
CSB Viegas, RM Costa, L Santos, PA Videira, Z Silva, N Araújo, AL Macedo, AP Matos, C Vermeer, DC Simes Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases PLoS ONE, 2017;12(5):e0177829. 2017 [PMID: 28542410] (Bioassay, Human) Bioassay Human
J Wang, H Ye, D Zhang, K Cheng, Y Hu, X Yu, L Lu, J Hu, C Zuo, B Qian, Y Yu, S Liu, G Liu, C Mao, S Liu Cancer-derived Circulating MicroRNAs Promote Tumor Angiogenesis by Entering Dendritic Cells to Degrade Highly Complementary MicroRNAs Theranostics, 2017;7(6):1407-1421. 2017 [PMID: 28529626] (Bioassay, Human) Bioassay Human
SC Silva-Card, AJ Affandi, L Spel, M Cossu, JAG van Roon, M Boes, TRDJ Radstake CXCL4 Exposure Potentiates TLR-Driven Polarization of Human Monocyte-Derived Dendritic Cells and Increases Stimulation of T Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28515281] (Bioassay, Human) Bioassay Human
Show All 259 Publications.

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Gene Symbol IL4