Recombinant Human IL-4 Protein, CF


1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-4 (Catalog # 204‑IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.05‑02 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-4 Protein, CF Summary

Additional Information
A New rh IL-4 is Now Available! The new protein is mammalian cell expressed!
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.
The specific activity of recombinant human IL-4 is approximately 2.9 x 104 IU/μg, which is calibrated against human IL-4 WHO International Standard (NIBSC code: 88/656).
E. coli-derived human IL-4 protein
His25-Ser153, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
14 kDa, reducing conditions
Read Publications using
204-IL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-4 Protein, CF

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra


Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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Publications for IL-4 (204-IL/CF)(290)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chinchilla, N/A, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, ELISA Standard, Ex Vivo, cell culture.

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cell culture
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Primate - Chlorocebus pygerythrus (Vervet Monkey)
Primate - Macaca mulatta (Rhesus Macaque)
All Species
Showing Publications 1 - 10 of 290. Show All 290 Publications.
Publications using 204-IL/CF Applications Species
T Yang, W Zhang, L Wang, C Xiao, L Wang, Y Gong, D Huang, B Guo, Q Li, Y Xiang, Y Nan Co-culture of dendritic cells and cytokine-induced killer cells effectively suppresses liver cancer stem cell growth by inhibiting pathways in the immune system BMC Cancer, 2018;18(1):984. 2018 [PMID: 30326865] (Bioassay, Human) Bioassay Human
J Hrdý, K Vlasáková, V ?erný, L Súkeníková, O Novotná, P Petrásková, K Boráková, R Lodinová-Ž, L Kolá?ová, L Prokešová Decreased allergy incidence in children supplemented with E. coli O83:K24:H31 and its possible modes of action Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 30306557] (Human) Human
L She, Y Qin, J Wang, C Liu, G Zhu, G Li, M Wei, C Chen, G Liu, D Zhang, X Chen, Y Wang, Y Qiu, Y Tian, X Zhang, Y Liu, D Huang Tumor-associated macrophages derived CCL18 promotes metastasis in squamous cell carcinoma of the head and neck Cancer Cell Int., 2018;18(0):120. 2018 [PMID: 30181713] (Bioassay, Human) Bioassay Human
CH Lu, DW Yeh, CY Lai, YL Liu, LR Huang, AY Lee, SC Jin, TH Chuang USP17 mediates macrophage-promoted inflammation and stemness in lung cancer cells by regulating TRAF2/TRAF3 complex formation Oncogene, 2018;0(0):. 2018 [PMID: 30038267] (Bioassay, Human) Bioassay Human
G Genard, AC Wera, C Huart, B Le Calve, S Penninckx, A Fattacciol, T Tabarrant, C Demazy, N Ninane, AC Heuskin, S Lucas, C Michiels Proton irradiation orchestrates macrophage reprogramming through NF?B signaling Cell Death Dis, 2018;9(7):728. 2018 [PMID: 29950610] (Bioassay, Human) Bioassay Human
L Ladislau, DM Portilho, T Courau, A Solares-Pé, E Negroni, J Lainé, D Klatzmann, A Bonomo, Y Allenbach, O Benveniste, I Riederer, W Savino, V Mouly, G Butler-Bro, CF Benjamim Activated dendritic cells modulate proliferation and differentiation of human myoblasts Cell Death Dis, 2018;9(5):551. 2018 [PMID: 29748534] (Bioassay, Human) Bioassay Human
LK Martin, A Hollaus, A Stahuber, C Hübener, A Fraccaroli, J Tischer, A Schub, A Moosmann Cross-sectional analysis of CD8 T cell immunity to human herpesvirus 6B PLoS Pathog., 2018;14(4):e1006991. 2018 [PMID: 29698478] (Bioassay, Human) Bioassay Human
EY Huang, JC Chang, HH Chen, CY Hsu, HC Hsu, KL Wu Carcinoembryonic antigen as a marker of radioresistance in colorectal cancer: a potential role of macrophages BMC Cancer, 2018;18(1):321. 2018 [PMID: 29580202] (Bioassay, Human) Bioassay Human
X Chen, S Hao, Z Zhao, J Liu, Q Shao, F Wang, D Sun, Y He, W Gao, H Mao Interleukin 35: Inhibitory regulator in monocyte-derived dendritic cell maturation and activation Cytokine, 2018;108(0):43-52. 2018 [PMID: 29571039] (Bioassay, Human) Bioassay Human
T Kato, T Matsuda, Y Ikeda, JH Park, M Leisegang, S Yoshimura, T Hikichi, M Harada, M Zewde, S Sato, K Hasegawa, K Kiyotani, Y Nakamura Effective screening of T cells recognizing neoantigens and construction of T-cell receptor-engineered T cells Oncotarget, 2018;9(13):11009-11019. 2018 [PMID: 29541393] (Bioassay, Human) Bioassay Human
Show All 290 Publications.

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Gene Symbol IL4