Recombinant Human IL-4 Protein, CF

Images

 
1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-4 (Catalog # 204‑IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.05‑02 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human IL-4 Protein, CF Summary

Additional Information
A New rhIL-4 is Now Available! The new protein is mammalian cell expressed!
New Product 6507-IL
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.
The specific activity of recombinant human IL-4 is approximately 2.9 x 104 IU/μg, which is calibrated against human IL-4 WHO International Standard (NIBSC code: 88/656).
Source
E. coli-derived human IL-4 protein
His25-Ser153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
Publications
Read Publications using
204-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-4 Protein, CF

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-25241
Species: Hu, Mu
Applications: WB, ICC/IF, IP
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NB600-922
Species: Ch, Vi
Applications: WB, ELISA, IHC, IHC-P, IP
NBP2-24867
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)

Publications for IL-4 (204-IL/CF)(286)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chinchilla, N/A, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, ELISA Standard, Ex Vivo, cell culture.


Filter By Application
Bioassay
(270)
Cell Culture Substrate
(2)
ELISA Standard
(8)
Ex Vivo
(1)
cell culture
(3)
All Applications
Filter By Species
Human
(262)
Mouse
(5)
Rat
(1)
Chinchilla
(1)
N/A
(8)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 286. Show All 286 Publications.
Publications using 204-IL/CF Applications Species
G Genard, AC Wera, C Huart, B Le Calve, S Penninckx, A Fattacciol, T Tabarrant, C Demazy, N Ninane, AC Heuskin, S Lucas, C Michiels Proton irradiation orchestrates macrophage reprogramming through NF?B signaling Cell Death Dis, 2018;9(7):728. 2018 [PMID: 29950610] (Bioassay, Human) Bioassay Human
L Ladislau, DM Portilho, T Courau, A Solares-Pé, E Negroni, J Lainé, D Klatzmann, A Bonomo, Y Allenbach, O Benveniste, I Riederer, W Savino, V Mouly, G Butler-Bro, CF Benjamim Activated dendritic cells modulate proliferation and differentiation of human myoblasts Cell Death Dis, 2018;9(5):551. 2018 [PMID: 29748534] (Bioassay, Human) Bioassay Human
LK Martin, A Hollaus, A Stahuber, C Hübener, A Fraccaroli, J Tischer, A Schub, A Moosmann Cross-sectional analysis of CD8 T cell immunity to human herpesvirus 6B PLoS Pathog., 2018;14(4):e1006991. 2018 [PMID: 29698478] (Bioassay, Human) Bioassay Human
EY Huang, JC Chang, HH Chen, CY Hsu, HC Hsu, KL Wu Carcinoembryonic antigen as a marker of radioresistance in colorectal cancer: a potential role of macrophages BMC Cancer, 2018;18(1):321. 2018 [PMID: 29580202] (Bioassay, Human) Bioassay Human
X Chen, S Hao, Z Zhao, J Liu, Q Shao, F Wang, D Sun, Y He, W Gao, H Mao Interleukin 35: Inhibitory regulator in monocyte-derived dendritic cell maturation and activation Cytokine, 2018;108(0):43-52. 2018 [PMID: 29571039] (Bioassay, Human) Bioassay Human
T Kato, T Matsuda, Y Ikeda, JH Park, M Leisegang, S Yoshimura, T Hikichi, M Harada, M Zewde, S Sato, K Hasegawa, K Kiyotani, Y Nakamura Effective screening of T cells recognizing neoantigens and construction of T-cell receptor-engineered T cells Oncotarget, 2018;9(13):11009-11019. 2018 [PMID: 29541393] (Bioassay, Human) Bioassay Human
E Berdyshev, E Goleva, I Bronova, N Dyjack, C Rios, J Jung, P Taylor, M Jeong, CF Hall, BN Richers, KA Norquest, T Zheng, MA Seibold, DY Leung Lipid abnormalities in atopic skin are driven by type 2 cytokines JCI Insight, 2018;3(4):. 2018 [PMID: 29467325] (Bioassay, Human) Bioassay Human
D Wang, XF Huang, B Hong, XT Song, L Hu, M Jiang, B Zhang, H Ning, Y Li, C Xu, X Lou, B Li, Z Yu, J Hu, J Chen, F Yang, H Gao, G Ding, L Liao, L Rollins, L Jones, SY Chen, H Chen Efficacy of intracellular immune checkpoint-silenced DC vaccine JCI Insight, 2018;3(3):. 2018 [PMID: 29415891] (Bioassay, Human) Bioassay Human
HD Brightbill, E Suto, N Blaquiere, N Ramamoorth, S Sujatha-Bh, EB Gogol, GM Castanedo, BT Jackson, YC Kwon, S Haller, J Lesch, K Bents, C Everett, PB Kohli, S Linge, L Christian, K Barrett, A Jaochico, LM Berezhkovs, PW Fan, Z Modrusan, K Veliz, MJ Townsend, J DeVoss, AR Johnson, R Godemann, WP Lee, CD Austin, BS McKenzie, JA Hackney, JJ Crawford, ST Staben, MH Alaoui Ism, LC Wu, N Ghilardi NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus Nat Commun, 2018;9(1):179. 2018 [PMID: 29330524] (Bioassay, Human) Bioassay Human
YJ Choi, SJ Park, YS Park, HS Park, KM Yang, K Heo EpCAM peptide-primed dendritic cell vaccination confers significant anti-tumor immunity in hepatocellular carcinoma cells PLoS ONE, 2018;13(1):e0190638. 2018 [PMID: 29298343] (Bioassay, Human) Bioassay Human
Show All 286 Publications.

Reviews for IL-4 (204-IL/CF) (0)

There are no reviews for IL-4 (204-IL/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for IL-4 (204-IL/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human IL-4 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol IL4
Entrez
Uniprot