Recombinant Human IL-4 Protein, CF

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1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-4 (Catalog # 204‑IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.05‑02 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human IL-4 Protein, CF Summary

Additional Information
A New rhIL-4 is Now Available! The new protein is mammalian cell expressed!
New Product 6507-IL
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.
The specific activity of recombinant human IL-4 is approximately 2.9 x 104 IU/μg, which is calibrated against human IL-4 WHO International Standard (NIBSC code: 88/656).
Source
E. coli-derived
His25-Ser153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
Publications
Read Publications using
204-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-4 Protein, CF

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

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Publications for (259)

We have publications tested in 7 confirmed species: Human, Mouse, Chinchilla, N/A, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, ELISA Standard, Ex Vivo, cell culture.


Filter By Application
Bioassay
(243)
Cell Culture Substrate
(2)
ELISA Standard
(8)
Ex Vivo
(1)
cell culture
(3)
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Filter By Species
Human
(237)
Mouse
(4)
Chinchilla
(1)
N/A
(8)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 259. Show All 259 Publications.
Publications using 204-IL/CF Applications Species
J Zeitvogel, N Jokmin, S Rieker, I Klug, C Brandenber, T Werfel GATA3 regulates FLG and FLG2 expression in human primary keratinocytes Sci Rep, 2017;7(1):11847. 2017 [PMID: 28928464] (Bioassay, Human) Bioassay Human
M Yoshida, Y Ishioka, T Ozawa, H Okuyama, M Iguchi, T Ota, T Ito, M Nagira, A Morita, H Tanaka, H Naito, H Kidoya, N Takakura Soluble HLA-associated peptide from PSF1 has a cancer vaccine potency Sci Rep, 2017;7(1):11137. 2017 [PMID: 28894200] (Bioassay, Human) Bioassay Human
L Sarmiento, J De Rosa, A Machicote, LA Billordo, P Baz, PM Fernández, I Kaimen Mac, A Blanco, EI Arana Characterization of tonsillar IL10 secreting B cells and their role in the pathophysiology of tonsillar hypertrophy Sci Rep, 2017;7(1):11077. 2017 [PMID: 28894116] (Bioassay, Human) Bioassay Human
J Helft, F Anjos-Afon, AG van der Ve, P Chakravart, D Bonnet, C Reis E Sou Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors Cell Rep, 2017;20(3):529-537. 2017 [PMID: 28723558] (Bioassay, Human) Bioassay Human
DJ Son, YY Jung, MH Park, HL Lee, MJ Song, HS Yoo, DY Hwang, SB Han, JT Hong Activated Natural Killer Cells Mediate the Suppressive Effect of Interleukin-4 on Tumor Development via STAT6 Activation in an Atopic Condition Melanoma Model Neoplasia, 2017;19(7):537-548. 2017 [PMID: 28587956] (Bioassay, Human) Bioassay Human
AM Clemente, G Castronovo, A Antonelli, MM D'Andrea, M Tanturli, E Perissi, S Paccosi, A Parenti, F Cozzolino, GM Rossolini, MG Torcia Differential Th17 response induced by the two clades of the pandemic ST258 Klebsiella pneumoniae clonal lineages producing KPC-type carbapenemase PLoS ONE, 2017;12(6):e0178847. 2017 [PMID: 28586386] (Bioassay, Human) Bioassay Human
JY Ao, XD Zhu, ZT Chai, H Cai, YY Zhang, KZ Zhang, LQ Kong, N Zhang, BG Ye, DN Ma, HC Sun Colony-stimulating factor 1 receptor blockade inhibits tumor growth by altering the polarization of tumor-associated macrophages in hepatocellular carcinoma Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28572167] (Bioassay, Human) Bioassay Human
CSB Viegas, RM Costa, L Santos, PA Videira, Z Silva, N Araújo, AL Macedo, AP Matos, C Vermeer, DC Simes Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases PLoS ONE, 2017;12(5):e0177829. 2017 [PMID: 28542410] (Bioassay, Human) Bioassay Human
J Wang, H Ye, D Zhang, K Cheng, Y Hu, X Yu, L Lu, J Hu, C Zuo, B Qian, Y Yu, S Liu, G Liu, C Mao, S Liu Cancer-derived Circulating MicroRNAs Promote Tumor Angiogenesis by Entering Dendritic Cells to Degrade Highly Complementary MicroRNAs Theranostics, 2017;7(6):1407-1421. 2017 [PMID: 28529626] (Bioassay, Human) Bioassay Human
SC Silva-Card, AJ Affandi, L Spel, M Cossu, JAG van Roon, M Boes, TRDJ Radstake CXCL4 Exposure Potentiates TLR-Driven Polarization of Human Monocyte-Derived Dendritic Cells and Increases Stimulation of T Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28515281] (Bioassay, Human) Bioassay Human
Show All 259 Publications.

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Bioinformatics

Gene Symbol IL4
Entrez
Uniprot