Recombinant Human IL-4 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-4 Protein Summary

Additional Information
A New rh IL-4 is Now Available! The new protein is mammalian cell expressed!
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.
The specific activity of recombinant human IL-4 is approximately 2.9 x 104 IU/μg, which is calibrated against human IL-4 WHO International Standard (NIBSC code: 88/656).
Source
E. coli-derived human IL-4 protein
His25-Ser153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
204-IL in the following application:

Publications
Read Publications using
204-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-4 Protein

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • IL4E12
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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Publications for IL-4 (204-IL)(349)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chinchilla, N/A, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 6 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), ELISA Standard, Ex Vivo.


Filter By Application
Bioassay
(311)
Cell Culture
(24)
Differentiation
(1)
ELISA (Standard)
(8)
ELISA Standard
(1)
Ex Vivo
(1)
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Filter By Species
Human
(325)
Mouse
(5)
Rat
(1)
Chinchilla
(1)
N/A
(8)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 349. Show All 349 Publications.
Publications using 204-IL Applications Species
KJ Gregory, SM Morin, A Kubosiak, J Ser-Dolans, B Schalet, DJ Jerry, SS Schneider The use of patient-derived breast tissue explants to study macrophage polarization and the effects of environmental chemical exposure Immunol. Cell Biol., 2020;0(0):. 2020 [PMID: 32713010] (Cell Culture, Human) Cell Culture Human
B Wang, K Hara, A Kawabata, M Nishimura, A Wakata, LH Tjan, AL Poetranto, C Yamamoto, Y Haseda, T Aoshi, L Munakata, R Suzuki, M Komatsu, R Tsukamoto, T Itoh, C Nishigori, Y Saito, T Matozaki, Y Mori Tetrameric glycoprotein complex gH/gL/gQ1/gQ2 is a promising vaccine candidate for human herpesvirus 6B PLoS Pathog., 2020;16(7):e1008609. 2020 [PMID: 32702057] (Cell Culture, Human) Cell Culture Human
K Shan, N Feng, J Cui, S Wang, H Qu, G Fu, J Li, H Chen, X Wang, R Wang, Y Qi, Z Gu, YQ Chen Resolvin D1 and D2 inhibit tumour growth and inflammation via modulating macrophage polarization J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32469149] (Bioassay, Human) Bioassay Human
J Lu, G Jiang, Y Wu, S Antony, JL Meitzler, A Juhasz, H Liu, K Roy, H Makhlouf, R Chuaqui, D Butcher, MM Konaté, JH Doroshow NADPH oxidase 1 is highly expressed in human large and small bowel cancers PLoS ONE, 2020;15(5):e0233208. 2020 [PMID: 32428030] (Bioassay, Human) Bioassay Human
YD Kim, SM Park, HC Ha, AR Lee, H Won, H Cha, S Cho, JM Cho HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment J Cancer, 2020;11(14):4059-4072. 2020 [PMID: 32368288] (Bioassay, Human) Bioassay Human
QM Nhu, L Hsieh, L Dohil, R Dohil, RO Newbury, R Kurten, FJ Moawad, SS Aceves Antifibrotic Effects of the Thiazolidinediones in Eosinophilic Esophagitis Pathologic Remodeling: A Preclinical Evaluation Clin Transl Gastroenterol, 2020;11(4):e00164. 2020 [PMID: 32352681] (Cell Culture, Human) Cell Culture Human
M Minopoli, S Sarno, G Di Carlucc, R Azzaro, S Costantini, F Fazioli, M Gallo, G Apice, L Cannella, D Rea, MP Stoppelli, D Boraschi, A Budillon, K Scotlandi, A De Chiara, MV Carriero Inhibiting Monocyte Recruitment to Prevent the Pro-Tumoral Activity of Tumor-Associated Macrophages in Chondrosarcoma Cells, 2020;9(4):. 2020 [PMID: 32344648] (Bioassay, Human) Bioassay Human
X Unamuno, J Gómez-Ambr, B Ramírez, A Rodríguez, S Becerril, V Valentí, R Moncada, C Silva, J Salvador, G Frühbeck, V Catalán Dermatopontin, A Novel Adipokine Promoting Adipose Tissue Extracellular Matrix Remodelling and Inflammation in Obesity J Clin Med, 2020;9(4):. 2020 [PMID: 32283761] (Cell Culture, Human) Cell Culture Human
H Gan, T Shen, DP Chupp, JR Taylor, HN Sanchez, X Li, Z Xu, H Zan, P Casali B cell Sirt1 deacetylates histone and non-histone proteins for epigenetic modulation of AID expression and the antibody response Sci Adv, 2020;6(14):eaay2793. 2020 [PMID: 32270032] (Cell Culture, Human) Cell Culture Human
A Papait, E Vertua, M Magatti, S Ceccarigli, S De Munari, AR Silini, M Sheleg, R Ofir, O Parolini Mesenchymal Stromal Cells from Fetal and Maternal Placenta Possess Key Similarities and Differences: Potential Implications for Their Applications in Regenerative Medicine Cells, 2020;9(1):. 2020 [PMID: 31935836] (Cell Culture, Human) Cell Culture Human
Show All 349 Publications.

Reviews for IL-4 (204-IL) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 204-IL:
Filter by Applications
CellProlif
(1)
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Images Ratings Applications Species Date Details
 IL-4 204-IL
Enlarge
5
reviewed by:
Anonymous
06/09/2019
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Summary

LotNCL0617091

Comments

CommentsCells supplied with 20ng/ml IL-4 and 100ng/ml of GM-CSF to differentiate dendritic cells
Cell Proliferation IL-4 204-IL
Enlarge
5
reviewed by:
Anonymous
CellProlif 10/07/2018
View

Summary

ApplicationCell Proliferation
LotNCL0617091

Comments

CommentsCells were supplied with the cytokine
treatments: 50 ng /ml GM-CSF and 10 ng/ ml1 of IL-4 to differentiate DC cells

FAQs for IL-4 (204-IL) (0)

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Blogs on IL-4.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

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Recent Reviews

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Anonymous
06/09/2019
Application:
Species:

Anonymous
10/07/2018
Application: CellProlif
Species:

Bioinformatics

Gene Symbol IL4
Uniprot