Recombinant Human IL-4 Protein


1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-4 (Catalog # 204-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.05-02 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-4 Protein Summary

Additional Information
A New rh IL-4 is Now Available! The new protein is mammalian cell expressed!
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.
The specific activity of recombinant human IL-4 is approximately 2.9 x 104 IU/μg, which is calibrated against human IL-4 WHO International Standard (NIBSC code: 88/656).
E. coli-derived human IL-4 protein
His25-Ser153, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
14 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
204-IL in the following application:

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204-IL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-4 Protein

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra


Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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Publications for IL-4 (204-IL)(307)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chinchilla, N/A, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), Ex Vivo.

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Primate - Chlorocebus pygerythrus (Vervet Monkey)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 307. Show All 307 Publications.
Publications using 204-IL Applications Species
S Márquez, JJ Fernández, C Mancebo, C Herrero-Sá, S Alonso, TA Sandoval, M Rodríguez, JR Cubillos-R, O Montero, N Fernández, M Sánchez Cr Tricarboxylic Acid Cycle Activity and Remodeling of Glycerophosphocholine Lipids Support Cytokine Induction in Response to Fungal Patterns Cell Rep, 2019;27(2):525-536.e4. 2019 [PMID: 30970255] (Bioassay, Human) Bioassay Human
J Kristoff, ML Palma, TM Garcia-Bat, C Shen, N Sluis-Crem, P Gupta, CR Rinaldo, RB Mailliard Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1 EBioMedicine, 2019;0(0):. 2019 [PMID: 30952614] (Bioassay, Human) Bioassay Human
R Gramlich, E Aliahmadi, M Peiser In Vitro Induction of T Helper 17 Cells by Synergistic Activation of Human Monocyte-Derived Langerhans Cell-Like Cells with Bacterial Agonists Int J Mol Sci, 2019;20(6):. 2019 [PMID: 30893757] (Bioassay, Human) Bioassay Human
TA Sebrell, M Hashimi, B Sidar, R Wilkinson, L Kirpotina, MT Quinn, Z Malkoç, PJ Taylor, JN Wilking, D Bimczok A Novel Gastric Spheroid Co-Culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 30878664] (Bioassay, Human) Bioassay Human
M Kita, Y Ano, A Inoue, J Aoki Identification of P2Y receptors involved in oleamide-suppressing inflammatory responses in murine microglia and human dendritic cells Sci Rep, 2019;9(1):3135. 2019 [PMID: 30816271] (Bioassay, Human) Bioassay Human
F Feng, J Zhao, P Li, R Li, L Chen, C Sun Preexisting Virus-Specific T Lymphocytes-Mediated Enhancement of Adenovirus Infections to Human Blood CD14+ Cells Viruses, 2019;11(2):. 2019 [PMID: 30781810] (Bioassay, Human) Bioassay Human
C Anzilotti, DJ Swan, B Boisson, M Deobagkar-, C Oliveira, P Chabosseau, KR Engelhardt, X Xu, R Chen, L Alvarez, R Berlinguer, KR Bull, E Cawthorne, AP Cribbs, TL Crockford, TS Dang, A Fearn, EJ Fenech, SJ de Jong, BC Lagerholm, CS Ma, D Sims, B van den Be, Y Xu, AJ Cant, G Kleiner, TR Leahy, MT de la More, JM Puck, RS Shapiro, M van der Bu, JR Chapman, JC Christians, B Davies, JA McGrath, S Przyborski, M Santibanez, SG Tangye, A Werner, GA Rutter, S Padilla-Pa, JL Casanova, RJ Cornall, ME Conley, S Hambleton An essential role for the Zn2+ transporter ZIP7 in B cell development Nat. Immunol., 2019;0(0):. 2019 [PMID: 30718914] (Bioassay, Human) Bioassay Human
M Pujol, C Borie, M Montoya, A Ferreira, R Vernal Brucella canis induces canine CD4+ T cells multi-cytokine Th1/Th17 production via dendritic cell activation Comp. Immunol. Microbiol. Infect. Dis., 2019;62(0):68-75. 2019 [PMID: 30711049] (Bioassay, Human) Bioassay Human
K Gettler, M Giri, E Kenigsberg, J Martin, LS Chuang, NY Hsu, LA Denson, JS Hyams, A Griffiths, JD Noe, WV Crandall, DR Mack, R Kellermaye, C Abraham, G Hoffman, S Kugathasan, JH Cho Prioritizing Crohn&#039;s disease genes by integrating association signals with gene expression implicates monocyte subsets Genes Immun., 2019;0(0):. 2019 [PMID: 30692607] (Bioassay, Human) Bioassay Human
X Liu, P Zhang, Y Zhang, Z Wang, S Xu, Y Li, W Huai, Q Zhou, X Chen, X Chen, N Li, P Wang, Y Li, X Cao Glycolipid iGb3 feedback amplifies innate immune responses via CD1d reverse signaling Cell Res., 2018;0(0):. 2018 [PMID: 30514903] (Bioassay, Human) Bioassay Human
Show All 307 Publications.

Reviews for IL-4 (204-IL) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 204-IL:
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 IL-4 204-IL
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CommentsCells supplied with 20ng/ml IL-4 and 100ng/ml of GM-CSF to differentiate dendritic cells
Cell Proliferation IL-4 204-IL
reviewed by:
CellProlif 10/07/2018


ApplicationCell Proliferation


CommentsCells were supplied with the cytokine
treatments: 50 ng /ml GM-CSF and 10 ng/ ml1 of IL-4 to differentiate DC cells

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Application: CellProlif


Gene Symbol IL4