Recombinant Human IL-4 Protein

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1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-4 (Catalog # 204-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.05-02 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-4 Protein Summary

Additional Information
A New rh IL-4 is Now Available! The new protein is mammalian cell expressed!
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.
The specific activity of recombinant human IL-4 is approximately 2.9 x 104 IU/μg, which is calibrated against human IL-4 WHO International Standard (NIBSC code: 88/656).
Source
E. coli-derived human IL-4 protein
His25-Ser153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
204-IL in the following application:

Publications
Read Publications using
204-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-4 Protein

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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204-IL
Species: Hu
Applications: Bioactivity

Publications for IL-4 (204-IL)(307)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chinchilla, N/A, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), Ex Vivo.


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Bioassay
(290)
Cell Culture
(5)
ELISA (Standard)
(8)
Ex Vivo
(1)
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Human
(283)
Mouse
(5)
Rat
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Chinchilla
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(8)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Rabbit
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Showing Publications 1 - 10 of 307. Show All 307 Publications.
Publications using 204-IL Applications Species
S Márquez, JJ Fernández, C Mancebo, C Herrero-Sá, S Alonso, TA Sandoval, M Rodríguez, JR Cubillos-R, O Montero, N Fernández, M Sánchez Cr Tricarboxylic Acid Cycle Activity and Remodeling of Glycerophosphocholine Lipids Support Cytokine Induction in Response to Fungal Patterns Cell Rep, 2019;27(2):525-536.e4. 2019 [PMID: 30970255] (Bioassay, Human) Bioassay Human
J Kristoff, ML Palma, TM Garcia-Bat, C Shen, N Sluis-Crem, P Gupta, CR Rinaldo, RB Mailliard Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1 EBioMedicine, 2019;0(0):. 2019 [PMID: 30952614] (Bioassay, Human) Bioassay Human
R Gramlich, E Aliahmadi, M Peiser In Vitro Induction of T Helper 17 Cells by Synergistic Activation of Human Monocyte-Derived Langerhans Cell-Like Cells with Bacterial Agonists Int J Mol Sci, 2019;20(6):. 2019 [PMID: 30893757] (Bioassay, Human) Bioassay Human
TA Sebrell, M Hashimi, B Sidar, R Wilkinson, L Kirpotina, MT Quinn, Z Malkoç, PJ Taylor, JN Wilking, D Bimczok A Novel Gastric Spheroid Co-Culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 30878664] (Bioassay, Human) Bioassay Human
M Kita, Y Ano, A Inoue, J Aoki Identification of P2Y receptors involved in oleamide-suppressing inflammatory responses in murine microglia and human dendritic cells Sci Rep, 2019;9(1):3135. 2019 [PMID: 30816271] (Bioassay, Human) Bioassay Human
F Feng, J Zhao, P Li, R Li, L Chen, C Sun Preexisting Virus-Specific T Lymphocytes-Mediated Enhancement of Adenovirus Infections to Human Blood CD14+ Cells Viruses, 2019;11(2):. 2019 [PMID: 30781810] (Bioassay, Human) Bioassay Human
C Anzilotti, DJ Swan, B Boisson, M Deobagkar-, C Oliveira, P Chabosseau, KR Engelhardt, X Xu, R Chen, L Alvarez, R Berlinguer, KR Bull, E Cawthorne, AP Cribbs, TL Crockford, TS Dang, A Fearn, EJ Fenech, SJ de Jong, BC Lagerholm, CS Ma, D Sims, B van den Be, Y Xu, AJ Cant, G Kleiner, TR Leahy, MT de la More, JM Puck, RS Shapiro, M van der Bu, JR Chapman, JC Christians, B Davies, JA McGrath, S Przyborski, M Santibanez, SG Tangye, A Werner, GA Rutter, S Padilla-Pa, JL Casanova, RJ Cornall, ME Conley, S Hambleton An essential role for the Zn2+ transporter ZIP7 in B cell development Nat. Immunol., 2019;0(0):. 2019 [PMID: 30718914] (Bioassay, Human) Bioassay Human
M Pujol, C Borie, M Montoya, A Ferreira, R Vernal Brucella canis induces canine CD4+ T cells multi-cytokine Th1/Th17 production via dendritic cell activation Comp. Immunol. Microbiol. Infect. Dis., 2019;62(0):68-75. 2019 [PMID: 30711049] (Bioassay, Human) Bioassay Human
K Gettler, M Giri, E Kenigsberg, J Martin, LS Chuang, NY Hsu, LA Denson, JS Hyams, A Griffiths, JD Noe, WV Crandall, DR Mack, R Kellermaye, C Abraham, G Hoffman, S Kugathasan, JH Cho Prioritizing Crohn&#039;s disease genes by integrating association signals with gene expression implicates monocyte subsets Genes Immun., 2019;0(0):. 2019 [PMID: 30692607] (Bioassay, Human) Bioassay Human
X Liu, P Zhang, Y Zhang, Z Wang, S Xu, Y Li, W Huai, Q Zhou, X Chen, X Chen, N Li, P Wang, Y Li, X Cao Glycolipid iGb3 feedback amplifies innate immune responses via CD1d reverse signaling Cell Res., 2018;0(0):. 2018 [PMID: 30514903] (Bioassay, Human) Bioassay Human
Show All 307 Publications.

Reviews for IL-4 (204-IL) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 204-IL:
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CellProlif
(1)
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Images Ratings Applications Species Date Details
 IL-4 204-IL
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reviewed by:
Anonymous
06/09/2019
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Summary

LotNCL0617091

Comments

CommentsCells supplied with 20ng/ml IL-4 and 100ng/ml of GM-CSF to differentiate dendritic cells
Cell Proliferation IL-4 204-IL
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reviewed by:
Anonymous
CellProlif 10/07/2018
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Summary

ApplicationCell Proliferation
LotNCL0617091

Comments

CommentsCells were supplied with the cytokine
treatments: 50 ng /ml GM-CSF and 10 ng/ ml1 of IL-4 to differentiate DC cells

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Anonymous
06/09/2019
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Anonymous
10/07/2018
Application: CellProlif
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Bioinformatics

Gene Symbol IL4
Uniprot