Recombinant Human IL-4 Protein

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1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-4 (Catalog # 204-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.05-02 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-4 Protein Summary

Additional Information
A New rh IL-4 is Now Available! The new protein is mammalian cell expressed!
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.
The specific activity of recombinant human IL-4 is approximately 2.9 x 104 IU/μg, which is calibrated against human IL-4 WHO International Standard (NIBSC code: 88/656).
Source
E. coli-derived human IL-4 protein
His25-Ser153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 5
using
204-IL in the following application:

Publications
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204-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-4 Protein

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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204-IL
Species: Hu
Applications: Bioactivity

Publications for IL-4 (204-IL)(290)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chinchilla, N/A, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, ELISA Standard, Ex Vivo, cell culture.


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Bioassay
(273)
Cell Culture Substrate
(2)
ELISA Standard
(8)
Ex Vivo
(1)
cell culture
(3)
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Human
(266)
Mouse
(5)
Rat
(1)
Chinchilla
(1)
N/A
(8)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 290. Show All 290 Publications.
Publications using 204-IL Applications Species
T Yang, W Zhang, L Wang, C Xiao, L Wang, Y Gong, D Huang, B Guo, Q Li, Y Xiang, Y Nan Co-culture of dendritic cells and cytokine-induced killer cells effectively suppresses liver cancer stem cell growth by inhibiting pathways in the immune system BMC Cancer, 2018;18(1):984. 2018 [PMID: 30326865] (Bioassay, Human) Bioassay Human
J Hrdý, K Vlasáková, V ?erný, L Súkeníková, O Novotná, P Petrásková, K Boráková, R Lodinová-Ž, L Kolá?ová, L Prokešová Decreased allergy incidence in children supplemented with E. coli O83:K24:H31 and its possible modes of action Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 30306557] (Human) Human
L She, Y Qin, J Wang, C Liu, G Zhu, G Li, M Wei, C Chen, G Liu, D Zhang, X Chen, Y Wang, Y Qiu, Y Tian, X Zhang, Y Liu, D Huang Tumor-associated macrophages derived CCL18 promotes metastasis in squamous cell carcinoma of the head and neck Cancer Cell Int., 2018;18(0):120. 2018 [PMID: 30181713] (Bioassay, Human) Bioassay Human
CH Lu, DW Yeh, CY Lai, YL Liu, LR Huang, AY Lee, SC Jin, TH Chuang USP17 mediates macrophage-promoted inflammation and stemness in lung cancer cells by regulating TRAF2/TRAF3 complex formation Oncogene, 2018;0(0):. 2018 [PMID: 30038267] (Bioassay, Human) Bioassay Human
G Genard, AC Wera, C Huart, B Le Calve, S Penninckx, A Fattacciol, T Tabarrant, C Demazy, N Ninane, AC Heuskin, S Lucas, C Michiels Proton irradiation orchestrates macrophage reprogramming through NF?B signaling Cell Death Dis, 2018;9(7):728. 2018 [PMID: 29950610] (Bioassay, Human) Bioassay Human
L Ladislau, DM Portilho, T Courau, A Solares-Pé, E Negroni, J Lainé, D Klatzmann, A Bonomo, Y Allenbach, O Benveniste, I Riederer, W Savino, V Mouly, G Butler-Bro, CF Benjamim Activated dendritic cells modulate proliferation and differentiation of human myoblasts Cell Death Dis, 2018;9(5):551. 2018 [PMID: 29748534] (Bioassay, Human) Bioassay Human
LK Martin, A Hollaus, A Stahuber, C Hübener, A Fraccaroli, J Tischer, A Schub, A Moosmann Cross-sectional analysis of CD8 T cell immunity to human herpesvirus 6B PLoS Pathog., 2018;14(4):e1006991. 2018 [PMID: 29698478] (Bioassay, Human) Bioassay Human
EY Huang, JC Chang, HH Chen, CY Hsu, HC Hsu, KL Wu Carcinoembryonic antigen as a marker of radioresistance in colorectal cancer: a potential role of macrophages BMC Cancer, 2018;18(1):321. 2018 [PMID: 29580202] (Bioassay, Human) Bioassay Human
X Chen, S Hao, Z Zhao, J Liu, Q Shao, F Wang, D Sun, Y He, W Gao, H Mao Interleukin 35: Inhibitory regulator in monocyte-derived dendritic cell maturation and activation Cytokine, 2018;108(0):43-52. 2018 [PMID: 29571039] (Bioassay, Human) Bioassay Human
T Kato, T Matsuda, Y Ikeda, JH Park, M Leisegang, S Yoshimura, T Hikichi, M Harada, M Zewde, S Sato, K Hasegawa, K Kiyotani, Y Nakamura Effective screening of T cells recognizing neoantigens and construction of T-cell receptor-engineered T cells Oncotarget, 2018;9(13):11009-11019. 2018 [PMID: 29541393] (Bioassay, Human) Bioassay Human
Show All 290 Publications.

Review for IL-4 (204-IL) (1) 51

Average Rating: 5
(Based on 1 review)

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 IL-4 204-IL
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reviewed by:
Anonymous
10/07/2018
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Summary

LotNCL0617091

Comments

CommentsCells were supplied with the cytokine
treatments: 50 ng /ml GM-CSF and 10 ng/ ml1 of IL-4 to differentiate DC cells

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Gene Symbol IL4
Uniprot