Recombinant Human IL-20 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human IL-20 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 0.2-0.6 ng/mL.
Source
E. coli-derived human IL-20 protein
Leu25-Glu176, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL20
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1102-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-20 Protein, CF

  • Cytokine Zcyto10
  • IL10D
  • IL20
  • IL-20
  • IL-20ZCYTO10four alpha helix cytokine
  • interleukin 20
  • interleukin-20
  • MGC96907

Background

Human Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences containing a signal sequence and amphipathic helices found in helical cytokines. Human IL-20 is synthesized as a 176 amino acid (aa) precursor with a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 share 77% aa sequence identity in their mature segments. Human IL-20 is active on mouse cells. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first is composed of IL-20 R alpha andIL-20 R beta. The second is composed of IL-22 R and IL-20 R beta. Whereas the IL-22 R/IL-20 R beta complex is shared with IL-24/mda-7, the IL-20 R alpha /IL-20 R beta  complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1 - 6).

  1. Blumberg, H. et al. (2001) Cell 104:9.
  2. Liu, L. et al. (2003) Blood 102:3206. 
  3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
  4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
  5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
  6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.

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Publications for IL-20 (1102-IL/CF)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(8)
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Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using 1102-IL/CF Applications Species
P Gough, S Ganesan, SK Datta IL-20 Signaling in Activated Human Neutrophils Inhibits Neutrophil Migration and Function J. Immunol., 2017;0(0):. 2017 [PMID: 28424238] (Bioassay, Human) Bioassay Human
Robert Sabat MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations Mediators Inflamm., 2016;2016(0):4097574. 2016 [PMID: 27843200] (Bioassay, Human) Bioassay Human
Addiction to the IGF2-ID1-IGF2 circuit for maintenance of the breast cancer stem-like cells Oncogene, 2016;0(0):. 2016 [PMID: 27546618] (Bioassay, Human) Bioassay Human
Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C Induction of phenotype modifying cytokines by FERMT1 mutations. Hum. Mutat., 2011;32(4):397-406. 2011 [PMID: 21309038] (Bioassay, Human) Bioassay Human
Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis. Eur. J. Immunol., 2009;39(12):3570-81. 2009 [PMID: 19830738] (Bioassay, Human) Bioassay Human
Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 2009;39(10):2779-88. 2009 [PMID: 19731362] (Bioassay, Human) Bioassay Human
Wolk K, Witte K, Witte E, Proesch S, Schulze-Tanzil G, Nasilowska K, Thilo J, Asadullah K, Sterry W, Volk HD, Sabat R Maturing dendritic cells are an important source of IL-29 and IL-20 that may cooperatively increase the innate immunity of keratinocytes. J. Leukoc. Biol., 2008;83(5):1181-93. 2008 [PMID: 18281438] (Bioassay, Human) Bioassay Human
Sa SM, Valdez PA, Wu J The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis. J. Immunol., 2007;178(4):2229-40. 2007 [PMID: 17277128] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol IL20
Entrez
Uniprot