Recombinant Human IL-22 Protein, CF


1 µg/lane of Recombinant Human IL-22 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-22 (Catalog # 782‑IL/CF) induces IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. The ED50 for this effect is 60‑300 pg/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-22 Protein, CF Summary

Details of Functionality
Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. Nagalakshmi, M.L. et al. (2004) International Immunopharmacology 4:679. The ED50 for this effect is 60-300 pg/mL.
E. coli-derived human IL-22 protein
Ala34-Ile179, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
782-IL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-22 Protein, CF

  • Cytokine Zcyto18
  • IL-10-related T-cell-derived inducible factor
  • IL-21
  • IL22
  • IL-22
  • IL-22IL21
  • IL-D110
  • IL-TIF
  • ILTIFIL-10-related T-cell-derived-inducible factor
  • IL-TIFMGC79382
  • interleukin 21
  • interleukin 22
  • interleukin-22
  • MGC79384
  • TIFa
  • TIFIL-23
  • zcyto18


Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT‑1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) PNAS 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

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Publications for IL-22 (782-IL/CF)(45)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 5 applications: Bioassay, ELISA Standard, ELISA capture, SPR, WB Standard.

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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
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Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using 782-IL/CF Applications Species
M Abikhair B, N Roudiani, J Chen, AL Santana, N Doudican, C Proby, D Felsen, JA Carucci Ruxolitinib inhibits cyclosporine-induced proliferation of cutaneous squamous cell carcinoma JCI Insight, 2018;3(17):. 2018 [PMID: 30185657] (Bioassay, Human) Bioassay Human
S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Bioassay, Human) Bioassay Human
R Bichele, J Kärner, K Truusalu, I Smidt, R Mändar, HR Conti, SL Gaffen, P Peterson, M Laan, K Kisand IL-22 neutralizing autoantibodies impair fungal clearance in murine oropharyngeal candidiasis model Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 29150834] (Bioassay, Mouse) Bioassay Mouse
Y Wang, JB Mumm, R Herbst, R Kolbeck, Y Wang IL-22 Increases Permeability of Intestinal Epithelial Tight Junctions by Enhancing Claudin-2 Expression J. Immunol., 2017;0(0):. 2017 [PMID: 28939759] (Bioassay, Human) Bioassay Human
M Tohyama, Y Shirakata, Y Hanakawa, X Dai, K Shiraishi, M Murakami, S Miyawaki, H Mori, R Utsunomiya, K Masuda, K Hashimoto, K Sayama Bcl-3 induced by IL-22 via STAT3 activation acts as a potentiator of psoriasis-related gene expression in epidermal keratinocytes Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 28901004] (Bioassay, Human) Bioassay Human
E Couderc, F Morel, P Levillain, A Buffière-M, M Camus, C Paquier, C Bodet, JF Jégou, M Pohin, L Favot, M Garcia, V Huguier, J Mcheik, C Lacombe, H Yssel, G Guillet, FX Bernard, JC Lecron Interleukin-17A-induced production of acute serum amyloid A by keratinocytes contributes to psoriasis pathogenesis PLoS ONE, 2017;12(7):e0181486. 2017 [PMID: 28708859] (Bioassay, Human) Bioassay Human
Y Kim, J Lee, J Kim, CW Choi, YI Hwang, JS Kang, WJ Lee The pathogenic role of interleukin-22 and its receptor during UVB-induced skin inflammation PLoS ONE, 2017;12(5):e0178567. 2017 [PMID: 28558005] (Bioassay, Human) Bioassay Human
S La Manna, PL Scognamigl, C Di Natale, M Leone, FA Mercurio, AM Malfitano, F Cianfarani, S Madonna, S Caravella, C Albanesi, E Novellino, D Marasco Characterization of linear mimetic peptides of Interleukin-22 from dissection of protein interfaces Biochimie, 2017;0(0):. 2017 [PMID: 28479106] (SPR, Human) SPR Human
J Zhang, IM Friberg, A Kift-Morga, G Parekh, MP Morgan, AR Liuzzi, CY Lin, KL Donovan, CS Colmont, PH Morgan, P Davis, I Weeks, DJ Fraser, N Topley, M Eberl Machine-learning algorithms define pathogen-specific local immune fingerprints in peritoneal dialysis patients with bacterial infections Kidney Int, 2017;0(0):. 2017 [PMID: 28318629] (ELISA Standard) ELISA Standard
JS Ainscough, T Macleod, D McGonagle, R Brakefield, JM Baron, A Alase, M Wittmann, M Stacey Cathepsin S is the major activator of the psoriasis-associated proinflammatory cytokine IL-36? Proc. Natl. Acad. Sci. U.S.A, 2017;114(13):E2748-E2757. 2017 [PMID: 28289191] (Bioassay, Human) Bioassay Human
Show All 45 Publications.

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Gene Symbol IL22