Recombinant Human IL-22 Protein, CF


Recombinant Human IL-22 (Catalog # 782‑IL/CF) induces IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. The ED50 for this effect is 60‑300 pg/mL.
1 µg/lane of Recombinant Human IL-22 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

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Applications Bioactivity

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Recombinant Human IL-22 Protein, CF Summary

Additional Information
E. coli
Details of Functionality
Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. Nagalakshmi, M.L. et al. (2004) International Immunopharmacology 4:679. The ED50 for this effect is 60-300 pg/mL.
E. coli-derived human IL-22 protein
Ala34-Ile179, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
782-IL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-22 Protein, CF

  • Cytokine Zcyto18
  • IL-10-related T-cell-derived inducible factor
  • IL22
  • IL-22
  • IL-D110
  • IL-TIF
  • ILTIFIL-10-related T-cell-derived-inducible factor
  • IL-TIFMGC79382
  • interleukin 22
  • interleukin-22
  • MGC79384
  • TIFa
  • TIFIL-23
  • zcyto18


Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta , share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT‑1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) PNAS 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

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Publications for IL-22 (782-IL/CF)(65)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 7 applications: Bioassay, Cell Culture, ELISA (Capture), ELISA (Standard), Stimulation, Surface Plasmon Resonance, Western Blot.

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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using 782-IL/CF Applications Species
C Maresca, G Di Maggio, C Stolfi, F Laudisi, M Colella, T Pacifico, A Di Grazia, D Di Fusco, D Congiu, AM Guida, G Sica, I Monteleone, G Monteleone Smad7 Sustains Stat3 Expression and Signaling in Colon Cancer Cells Cancers, 2022-10-12;14(20):. 2022-10-12 [PMID: 36291778] (Stimulation, Human) Stimulation Human
S Segarra, T Naiken, J Garnier, V Hamon, N Coussay, FX Bernard Enhanced In Vitro Expression of Filaggrin and Antimicrobial Peptides Following Application of Glycosaminoglycans and a Sphingomyelin-Rich Lipid Extract Oncogene, 2022-06-27;9(7):. 2022-06-27 [PMID: 35878340] (Bioassay, Human) Bioassay Human
DJ Carroll, MWN Burns, L Mottram, DC Propheter, A Boucher, GM Lessen, A Kumar, SA Malaker, C Xing, LV Hooper, U Yrlid, JJ Kohler Interleukin-22 regulates B3GNT7 expression to induce fucosylation of glycoproteins in intestinal epithelial cells The Journal of Biological Chemistry, 2021-12-02;0(0):101463. 2021-12-02 [PMID: 34864058] (Bioassay, Human) Bioassay Human
L Mercurio, M Morelli, C Scarponi, GL Scaglione, S Pallotta, C Albanesi, S Madonna PI3Kdelta Sustains Keratinocyte Hyperproliferation and Epithelial Inflammation: Implications for a Topically Druggable Target in Psoriasis Cells, 2021-10-02;10(10):. 2021-10-02 [PMID: 34685616] (Stimulation, Human) Stimulation Human
F Abdallah, E Henriet, A Suet, A Arar, R Clemençon, JM Malinge, G Lecellier, P Baril, C Pichon miR-21-3p/IL-22 Axes Are Major Drivers of Psoriasis Pathogenesis by Modulating Keratinocytes Proliferation-Survival Balance and Inflammatory Response Cells, 2021-09-26;10(10):. 2021-09-26 [PMID: 34685526] (Stimulation, Human) Stimulation Human
HC Hawerkamp, A Domdey, L Radau, P Sewerin, P Oláh, B Homey, S Meller Tofacitinib downregulates antiviral immune defence in keratinocytes and reduces T cell activation Arthritis Research & Therapy, 2021-05-21;23(1):144. 2021-05-21 [PMID: 34020693] (Bioassay, Human) Bioassay Human
HK Min, JY Won, BM Kim, KA Lee, SJ Lee, SH Lee, HR Kim, KW Kim Interleukin (IL)-25 suppresses IL-22-induced osteoclastogenesis in rheumatoid arthritis via STAT3 and p38 MAPK/I&amp;kappaB&amp;alpha pathway Arthritis Research & Therapy, 2020-09-23;22(1):222. 2020-09-23 [PMID: 32972460] (Human) Human
EY Kim, HM Noh, B Choi, JE Park, JE Kim, Y Jang, HK Lee, EJ Chang Interleukin-22 Induces the Infiltration of Visceral Fat Tissue by a Discrete Subset of Duffy Antigen Receptor for Chemokine-Positive M2-Like Macrophages in Response to a High Fat Diet Cells, 2019-12-06;8(12):. 2019-12-06 [PMID: 31817755] (Bioassay, Human, Mouse) Bioassay Human, Mouse
X Xu, H Fukui, Y Ran, X Wang, Y Inoue, N Ebisudani, H Nishimura, T Tomita, T Oshima, J Watari, H Kiyama, H Miwa The Link between Type III Reg and STAT3-Associated Cytokines in Inflamed Colonic Tissues Mediators Inflamm., 2019-11-06;2019(0):7859460. 2019-11-06 [PMID: 31780871] (Bioassay, Human) Bioassay Human
KD Hebert, N Mclaughlin, M Galeas-Pen, Z Zhang, T Eddens, A Govero, JM Pilewski, JK Kolls, DA Pociask Targeting the IL-22/IL-22BP axis enhances tight junctions and reduces inflammation during influenza infection Mucosal Immunol, 2019-10-09;0(0):. 2019-10-09 [PMID: 31597930] (Bioassay, Human) Bioassay Human
Show All 65 Publications.

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Gene Symbol IL22