Recombinant Human IL-17A (Human Cell-expressed) Protein

Recombinant Human IL-17 (Catalog # 7955-IL) induces CXCL1/GRO alpha secretion in HT‑29human colon adenocarcinoma cells. The ED50 for this effect is 0.12‑1.2 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human IL-17A (Human Cell-expressed) Protein Summary

• Details of Functionality: Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED 50 for this effect is 0.12-1.2 ng/mL. Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED 50 for this effect is 1.5-7.5 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein Gly24-Ala155
• Accession #: Q16552
• N-terminal Sequence: Gly24
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL17A
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 15.1 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15-23 kDa, reducing conditions 28-38 kDa, non-reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100-500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17A (Human Cell-expressed) Protein

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17A shares 60% amino acid sequence identity with mouse and rat IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17 RA in complex with IL-17 RC or IL-17 RD (7, 8). Both IL-17 RA and IL-17 RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9-11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15). IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).

1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
3. Yao, Z. et al. (1995) J. Immunol. 155:5483.
4. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
8. Rong, Z. et al. (2009) Cell Res. 19:208.
9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
13. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
14. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
15. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
16. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
17. Kryczek, I. et al. (2009) Blood 114:357.

Publications for IL-17/IL-17A (7955-IL)(17)

Publications using 7955-IL

• Sigutova, V;Xiang, W;Regensburger, M;Winner, B;Prots, I; Alpha-synuclein fine-tunes neuronal response to pro-inflammatory cytokines Brain, behavior, and immunity 2024-08-14 [PMID: 39128571] (Bioassay, Human)
• Ben, J;Moududee, S;Ge, D;Zhou, P;Wang, A;Liu, Y;You, Z; SCFFBXW11 Complex Targets Interleukin-17 Receptor A for Ubiquitin-Proteasome-Mediated Degradation Biomedicines 2024-03-28 [PMID: 38672111] (Bioassay, Human)
• Cao, QT;Ishak, M;Shpilman, I;Hirota, JA; TNF-? and Poly(I:C) induction of A20 and activation of NF-?B signaling are independent of ABCF1 in human airway epithelial cells Scientific reports 2023-09-07 [PMID: 37679460] (Bioassay, Human)
• P Pavlidis, A Tsakmaki, A Treveil, K Li, D Cozzetto, F Yang, U Niazi, BH Hayee, M Saqi, J Friedman, T Korcsmaros, G Bewick, N Powell Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease Cell Reports, 2022-09-27;40(13):111439. 2022-09-27 [PMID: 36170836] (Bioassay, Human)
• G Margetts, S Kleidonas, NS Zaibi, MS Zaibi, KD Edwards Evidence for anti-inflammatory effects and modulation of neurotransmitter metabolism by Salvia officinalis L BMC complementary medicine and therapies, 2022-05-12;22(1):131. 2022-05-12 [PMID: 35550086] (Bioassay, Human)
• EM Byrne, M Llorián-Sa, M Tang, A Margariti, M Chen, H Xu IL-17A Damages the Blood-Retinal Barrier through Activating the Janus Kinase 1 Pathway Biomedicines, 2021-07-16;9(7):. 2021-07-16 [PMID: 34356895] (Bioassay, Human)
• D Sinkeviciu, A Aspberg, Y He, AC Bay-Jensen, P Önnerfjord Characterization of the interleukin-17 effect on articular cartilage in a translational model: an explorative study BMC Rheumatol, 2020-05-12;4(0):30. 2020-05-12 [PMID: 32426694] (Bioassay, Bovine)
• L Sun, M Girnary, L Wang, Y Jiao, E Zeng, K Mercer, J Zhang, JT Marchesan, N Yu, K Moss, YL Lei, S Offenbache, S Zhang IL-10 Dampens an IL-17-Mediated Periodontitis-Associated Inflammatory Network J. Immunol., 2020-03-13;204(8):2177-2191. 2020-03-13 [PMID: 32169848] (Bioassay, Human)
• JE Heath, GM Scholz, PD Veith, EC Reynolds IL-36? regulates mediators of tissue homeostasis in epithelial cells Cytokine, 2019-03-08;119(0):24-31. 2019-03-08 [PMID: 30856602] (Bioassay, Human)
• S Muntaka, Y Almuhanna, D Jackson, S Singh, A Afryie-Asa, M Cámara, L Martínez-P IFN-? and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection Infect. Immun., 2019-01-24;0(0):. 2019-01-24 [PMID: 30455194] (Bioassay, Human)
• J Chorachoo, S Lambert, T Furnholm, L Roberts, L Reingold, S Auepemkiat, SP Voravuthik, A Johnston The small molecule rhodomyrtone suppresses TNF-? and IL-17A-induced keratinocyte inflammatory responses: A potential new therapeutic for psoriasis PLoS ONE, 2018-10-15;13(10):e0205340. 2018-10-15 [PMID: 30321197] (Bioassay, Human)
• ED Anderson, I Sastalla, NJ Earland, M Mahnaz, IN Moore, F Otaizo-Car, TG Myers, CA Myles, SK Datta, IA Myles Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632 PLoS ONE, 2018-09-12;13(9):e0198862. 2018-09-12 [PMID: 30208113] (Bioassay, Human)
• M Bambouskov, L Gorvel, V Lampropoul, A Sergushich, E Loginichev, K Johnson, D Korenfeld, ME Mathyer, H Kim, LH Huang, D Duncan, H Bregman, A Keskin, A Santeford, RS Apte, R Sehgal, B Johnson, GK Amarasingh, MP Soares, T Satoh, S Akira, T Hai, C de Guzman, K Auclair, TP Roddy, SA Biller, M Jovanovic, E Klechevsky, KM Stewart, GJ Randolph, MN Artyomov Electrophilic properties of itaconate and derivatives regulate the�I?B?-ATF3 inflammatory axis Nature, 2018-04-18;556(7702):501-504. 2018-04-18 [PMID: 29670287] (Bioassay, Human)
• BA Stanfield, PJF Rider, J Caskey, F Del Piero, KG Kousoulas Intramuscular vaccination of guinea pigs with the live-attenuated human herpes simplex vaccine VC2 stimulates a transcriptional profile of vaginal Th17 and regulatory Tr1 responses Vaccine, 2018-04-11;0(0):. 2018-04-11 [PMID: 29655629] (Cell Culture, Human)
• BK Tusi, SL Wolock, C Weinreb, Y Hwang, D Hidalgo, R Zilionis, A Waisman, JR Huh, AM Klein, M Socolovsky Population snapshots predict early haematopoietic and erythroid hierarchies Nature, 2018-02-21;0(0):. 2018-02-21 [PMID: 29466336] (Bioassay, Human)
• WR Swindell, MA Beamer, MK Sarkar, S Loftus, J Fullmer, X Xing, NL Ward, LC Tsoi, MJ Kahlenberg, Y Liang, JE Gudjonsson RNA-Seq Analysis of IL-1B and IL-36 Responses in Epidermal Keratinocytes Identifies a Shared MyD88-Dependent Gene Signature Front Immunol, 2018-01-29;9(0):80. 2018-01-29 [PMID: 29434599] (Cell Culture, Human)
• Impaired Tight Junctions in Atopic Dermatitis Skin and in a Skin-Equivalent Model Treated with Interleukin-17 PLoS ONE, 2016-09-02;11(9):e0161759. 2016-09-02 [PMID: 27588419] (Bioassay, Human)

FAQs for IL-17/IL-17A (7955-IL). (Showing 1 - 2 of 2 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A
• Uniprot:
  • Q16552()