Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF Summary

• Details of Functionality: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.4‑2 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-17A/F Heterodimer protein
  

  Human IL-17A (Gly24-Ala155) Accession # Q16552

  Human IL-17F (Arg31-Gln163) Accession # Q96PD4
  N-terminus	C-terminus

• Accession #: Q16552 (IL-17A) & Q96PD4 (IL-17F)
• N-terminal Sequence: Gly24 (IL-17A) & Arg21 (IL-17F)
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15.1 kDa (IL-17A) & 14.9 kDa (IL-17F).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20 & 16 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in 4 mM HCI.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF

• CTLA8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• Cytotoxic T-Lymphocyte-Associated Protein 8
• IL17
• IL-17
• IL-17A
• IL-17A/F Heterodimer
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A
• interleukin-17A

Background

Human IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (3-5). Mature human IL-17A and IL-17F share 61% and 56% amino acid sequence identity with mouse IL-17A and IL-17F, respectively. They share 50% aa sequence identity with each other. IL-17A/F and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
2. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
4. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
5. Gu, C. et al. (2013) Cytokine 64:477.
6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
7. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
8. Ouyang, W. et al. (2008) Immunity 28:454.
9. Steinman, L. (2007) Nat. Med. 13:139.
10. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
11. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
13. Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.

Publications for IL-17A/F Heterodimer (5837-IL)(1)

Publication using 5837-IL

• Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage. Eur J Immunol, 2015-12-14;46(2):440-5. 2015-12-14 [PMID: 26558442] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • Q16552 (IL-17A) & Q96PD4 (IL-17F)()