Recombinant Human IFN-gamma Protein, CF


Recombinant Human IFN-gamma (Catalog # 285‑IF/CF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater more
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IFN-gamma Protein, CF Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
E. coli-derived human IFN-gamma protein
Gln24-Gln166 with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
17 kDa, reducing conditions
Read Publications using
285-IF/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile, deionized water. 


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma Protein, CF

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma


Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma , 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma , and 37%‑43% with cotton rat, mouse, and rat IFN-gamma . IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Mu
Applications: ELISA
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Species: Hu
Applications: BA, PAGE
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
Species: Mu
Applications: ELISA
Species: Mu
Applications: ELISA
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: Bioactivity

Publications for IFN-gamma (285-IF/CF)(420)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Hamster, N/A, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 12 applications: Binding Assay, Bio-assay, Bioassay, Cell Culture, Differentiation, ELISA (Capture), ELISA (Standard), ELISA Standard, In Vivo, Stimulation, Western Blot, c.

Filter By Application
Binding Assay
Cell Culture
ELISA (Capture)
ELISA (Standard)
ELISA Standard
In Vivo
Western Blot
All Applications
Filter By Species
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
All Species
Showing Publications 1 - 10 of 420. Show All 420 Publications.
Publications using 285-IF/CF Applications Species
H Asashima, S Mohanty, M Comi, WE Ruff, KB Hoehn, P Wong, J Klein, C Lucas, I Cohen, S Coffey, N Lele, L Greta, K Raddassi, O Chaudhary, A Unterman, B Emu, SH Kleinstein, RR Montgomery, A Iwasaki, CS Dela Cruz, N Kaminski, AC Shaw, DA Hafler, TS Sumida PD-1highCXCR5-CD4+ peripheral helper T�cells promote CXCR3+ plasmablasts in human acute viral infection Cell Reports, 2022;0(0):111895. 2022 [PMID: 36596303] (Cell Culture, Human) Cell Culture Human
E Turner, L Chen, JG Foulke, Z Gu, F Tian CRISPR/Cas9 Edited RAS &amp; MEK Mutant Cells Acquire BRAF and MEK Inhibitor Resistance with MEK1 Q56P Restoring Sensitivity to MEK/BRAF Inhibitor Combo and KRAS G13D Gaining Sensitivity to Immunotherapy Cancers, 2022;14(21):. 2022 [PMID: 36358868] (Bioassay, Human) Bioassay Human
S Hayashi, CK Muraleedha, M Oku, S Tomar, SP Hogan, M Quiros, CA Parkos, A Nusrat Intestinal epithelial BLT1 promotes mucosal repair JCI Insight, 2022;0(0):. 2022 [PMID: 36301666] (Bioassay, Human) Bioassay Human
P Pavlidis, A Tsakmaki, A Treveil, K Li, D Cozzetto, F Yang, U Niazi, BH Hayee, M Saqi, J Friedman, T Korcsmaros, G Bewick, N Powell Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease Cell Reports, 2022;40(13):111439. 2022 [PMID: 36170836] (Bioassay, Human) Bioassay Human
S Mitra, R Gera, J Sundheimer, M Lemee, LU Wahlberg, B Linderoth, M Eriksdotte, H Behbahani Microglia Impairs Proliferation and Induces Senescence In-Vitro in NGF Releasing Cells Used in Encapsulated Cell Biodelivery for Alzheimer&#039;s Disease Therapy International Journal of Molecular Sciences, 2022;23(16):. 2022 [PMID: 36012296] (Bioassay, Human) Bioassay Human
T Meyer, D Shimon, S Youssef, G Yankovitz, A Tessler, T Chernobyls, A Gaoni-Yoge, R Perelroize, N Budick-Har, L Steinman, L Mayo NAD+ metabolism drives astrocyte proinflammatory reprogramming in central nervous system autoimmunity Proceedings of the National Academy of Sciences of the United States of America, 2022;119(35):e2211310119. 2022 [PMID: 35994674] (Cell Culture, Human) Cell Culture Human
S Chandrasek, JA Kochanowsk, EF Merritt, JS Lagas, A Swannigan, AA Koshy IFN-gamma stimulated murine and human neurons mount anti-parasitic defenses against the intracellular parasite Toxoplasma gondii Nature Communications, 2022;13(1):4605. 2022 [PMID: 35941154] (Bioassay, Human) Bioassay Human
Z Zhang, Y Xun, S Rong, L Yan, JA SoRelle, X Li, M Tang, K Keller, S Ludwig, EMY Moresco, B Beutler Loss of immunity-related GTPase GM4951 leads to nonalcoholic fatty liver disease without obesity Nature Communications, 2022;13(1):4136. 2022 [PMID: 35842425] (Bioassay, Human) Bioassay Human
S Padmanabha, B Gaire, Y Zou, MM Uddin, I Vancurova IFNgamma-induced PD-L1 expression in ovarian cancer cells is regulated by JAK1, STAT1 and IRF1 signaling Cellular Signalling, 2022;97(0):110400. 2022 [PMID: 35820543] (Bioassay, Human) Bioassay Human
XJ Mi, JK Kim, S Lee, SK Moon, YJ Kim, H Kim In vitro assessment of the anti-inflammatory and skin-moisturizing effects of Filipendula palmata (Pall.) Maxim. On human keratinocytes and identification of its bioactive phytochemicals Journal of ethnopharmacology, 2022;296(0):115523. 2022 [PMID: 35809756] (Bioassay, Human) Bioassay Human
Show All 420 Publications.

Reviews for IFN-gamma (285-IF/CF) (0)

There are no reviews for IFN-gamma (285-IF/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IFN-gamma (285-IF/CF). (Showing 1 - 1 of 1 FAQs).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

Additional IFN-gamma Products

Bioinformatics Tool for IFN-gamma (285-IF/CF)

Discover related pathways, diseases and genes to IFN-gamma (285-IF/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IFN-gamma (285-IF/CF)

Discover more about diseases related to IFN-gamma (285-IF/CF).

Pathways for IFN-gamma (285-IF/CF)

View related products by pathway.

PTMs for IFN-gamma (285-IF/CF)

Learn more about PTMs related to IFN-gamma (285-IF/CF).

Blogs on IFN-gamma.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.

  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human IFN-gamma Protein, CF and receive a gift card or discount.


Gene Symbol IFNG