Recombinant Human IFN-gamma Protein, CF


Recombinant Human IFN-gamma (Catalog # 285‑IF/CF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater more
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-gamma Protein, CF Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of Recombinant Human IFN-gamma is approximately 2 x 104 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).   Specific activity is for reference purposes only and is not routinely tested.  
E. coli-derived human IFN-gamma protein
Gln24-Gln166 with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
17 kDa, reducing conditions
Read Publications using
285-IF/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile, deionized water. 


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma Protein, CF

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma


Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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Publications for IFN-gamma (285-IF/CF)(352)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, ELISA (Standard), ELISA Standard, In Vivo, Western Blot, c.

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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
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Showing Publications 1 - 10 of 352. Show All 352 Publications.
Publications using 285-IF/CF Applications Species
AC Tecalco-Cr, M Macías-Sil, JO Ramírez-Ja, B Méndez-Amb Identification of genes modulated by interferon gamma in breast cancer cells Biochemistry and Biophysics Reports, 2021;27(0):101053. 2021 [PMID: 34189281] (Cell Culture, Human) Cell Culture Human
R Abreu, L Essler, P Giri, F Quinn Interferon-gamma promotes iron export in human macrophages to limit intracellular bacterial replication PLoS ONE, 2020;15(12):e0240949. 2020 [PMID: 33290416] (Cell Culture, Human) Cell Culture Human
X Li, G Yin, W Ji, J Liu, Y Zhang, J Wang, X Zhu, L Zhu, D Dai, W Ma, W Xu 18F-FHBG PET-CT Reporter Gene Imaging of Adoptive CIK Cell Transfer Immunotherapy for Breast Cancer in a Mouse Model Onco Targets Ther, 2020;13(0):11659-11668. 2020 [PMID: 33223839] (Cell Culture, Human) Cell Culture Human
V Panagiotak, D Ivanyuk, S De Cicco, W Haq, A Arsi?, C Yu, D Messelodi, M Oldrati, DC Schöndorf, MJ Perez, RP Cassatella, M Jakobi, N Schneiderh, T Gasser, I Niki?-Spie, M Deleidi Interferon-&amp;gamma signaling synergizes with LRRK2 in neurons and microglia derived from human induced pluripotent stem cells Nat Commun, 2020;11(1):5163. 2020 [PMID: 33057020] (Bioassay, Human) Bioassay Human
J Bhushan, JB Radke, YC Perng, M Mcallaster, DJ Lenschow, HW Virgin, LD Sibley ISG15 Connects Autophagy and IFN-&amp;gamma-Dependent Control of Toxoplasma gondii Infection in Human Cells MBio, 2020;11(5):. 2020 [PMID: 33024031] (Cell Culture, Human) Cell Culture Human
T Nicotra, A Desnos, J Halimi, H Antonot, L Reppel, T Belmas, A Freton, F Stranieri, M Mebarki, J Larghero, A Cras, L Faivre Mesenchymal stem/stromal cell quality control: validation of mixed lymphocyte reaction assay using flow cytometry according to ICH Q2(R1) Stem Cell Res Ther, 2020;11(1):426. 2020 [PMID: 33004063] (Cell Culture, Human) Cell Culture Human
B Cioni, A Zaalberg, JR van Beijnu, MHM Melis, J van Burgst, MJ Muraro, E Hooijberg, D Peters, I Hofland, Y Lubeck, J de Jong, J Sanders, J Vivié, HG van der Po, JP de Boer, AW Griffioen, W Zwart, AM Bergman Androgen receptor signalling in macrophages promotes TREM-1-mediated prostate cancer cell line migration and invasion Nat Commun, 2020;11(1):4498. 2020 [PMID: 32908142] (Bioassay, Human) Bioassay Human
F Duan, L Guo, L Yang, Y Han, A Thakur, BE Nilsson-Pa, P Wang, Z Zhang, CY Ma, X Zhou, T Han, T Zhang, X Wang, D Xu, X Duan, J Xiang, HF Tse, C Liao, W Luo, FP Huang, YW Chen, T Evans, RE Schwartz, B tenOever, DD Ho, S Chen, Q Lian, HJ Chen Modeling COVID-19 with Human Pluripotent Stem Cell-Derived Cells Reveals Synergistic Effects of Anti-inflammatory Macrophages with ACE2 Inhibition Against SARS-CoV-2 Res Sq, 2020;0(0):. 2020 [PMID: 32839764] (Cell Culture, Human) Cell Culture Human
E Abu-Shah, N Trendel, P Kruger, J Nguyen, J Pettmann, M Kutuzov, O Dushek Human CD8+ T Cells Exhibit a Shared Antigen Threshold for Different Effector Responses J. Immunol., 2020;0(0):. 2020 [PMID: 32817332] (Bioassay, Human) Bioassay Human
D Fisch, B Clough, MC Domart, V Encheva, H Bando, AP Snijders, LM Collinson, M Yamamoto, AR Shenoy, EM Frickel Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death Cell Rep, 2020;32(6):108008. 2020 [PMID: 32783936] (Bioassay, Human) Bioassay Human
Show All 352 Publications.

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FAQs for IFN-gamma (285-IF/CF). (Showing 1 - 1 of 1 FAQs).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

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Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.

  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
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Gene Symbol IFNG