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Recombinant Human ICAM-1/CD54 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human ICAM-1/CD54 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to Recombinant Human ICAM‑1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >30% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ICAM-1/CD54 protein
Met1-Glu480
Accession #
N-terminal Sequence
Not obtained, Predicted Gln28
Protein/Peptide Type
Recombinant Proteins
Gene
ICAM1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70-90 kDa, reducing conditions
Publications
Read Publications using
ADP4 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, containing Calcium and Magnesium with Sorbitol and with Trehalose.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ICAM-1/CD54 Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

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Publications for ICAM-1/CD54 (ADP4)(31)

We have publications tested in 4 confirmed species: Human, Mouse, Bacteria, N/A.

We have publications tested in 7 applications: Adhesion Assay, Affinity Purification, Bioassay, ELISA (Capture), ELISA (Standard), ELISA Capture, Surface Plasmon Resonance.


Filter By Application
Adhesion Assay
(1)
Affinity Purification
(1)
Bioassay
(24)
ELISA (Capture)
(1)
ELISA (Standard)
(2)
ELISA Capture
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(25)
Mouse
(1)
Bacteria
(1)
N/A
(1)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using ADP4 Applications Species
Lacouture, C;Chaves, B;Guipouy, D;Houmadi, R;Duplan-Eche, V;Allart, S;Destainville, N;Dupré, L; LFA-1 nanoclusters integrate TCR stimulation strength to tune T-cell cytotoxic activity Nature communications 2024-01-09 [PMID: 38195629] (Bioassay, Human) Bioassay Human
Sindram, E;Caballero-Oteyza, A;Kogata, N;Huang, S;Alizadeh, Z;Gamez-Díaz, L;Fazlollahi, MR;Peng, X;Grimbacher, B;Way, M;Proietti, M; ARPC5 deficiency leads to severe early onset systemic inflammation and mortality Disease models & mechanisms 2023-06-29 [PMID: 37382373] (Bioassay, Human) Bioassay Human
L Kaltenbach, P Martzloff, SK Bambach, N Aizarani, M Mihlan, A Gavrilov, KM Glaser, M Stecher, R Thünauer, A Thiriot, K Heger, K Kierdorf, S Wienert, UH von Andria, M Schmidt-Su, C Nerlov, F Klauschen, A Roers, M Bajénoff, D Grün, T Lämmermann Slow integrin-dependent migration organizes networks of tissue-resident mast cells Nature Immunology, 2023-04-20;0(0):. 2023-04-20 [PMID: 37081147] (Bioassay, Mouse) Bioassay Mouse
C Weichwald, I Zettl, I Ellinger, K Niespodzia, EE Waltl, S Villazala-, D Ivanov, J Eckl-Dorna, V Niederberg, R Valenta, S Flicker Antibody Conjugates Bispecific for Pollen Allergens and ICAM-1 with Potential to Prevent Epithelial Allergen Transmigration and Rhinovirus Infection International Journal of Molecular Sciences, 2023-02-01;24(3):. 2023-02-01 [PMID: 36769047] (ELISA Capture, Human, N/A) ELISA Capture Human, N/A
R Fitterer, T Lajqi, SA Kranig, M Braun, N Theissig, N Kuss, J Pöschl, D Frommhold, H Hudalla L-Arginine Modulates Neonatal Leukocyte Recruitment in a Gestational Age-Dependent Manner J Clin Med, 2020-08-27;9(9):. 2020-08-27 [PMID: 32867030] (Bioassay, Human) Bioassay Human
J Gamara, L Davis, E Rollet-Lab, T Hongu, Y Funakoshi, Y Kanaho, F Aoudjit, SG Bourgoin Assessment of Arf6 Deletion in PLB-985 Differentiated in Neutrophil-Like Cells and in Mouse Neutrophils: Impact on Adhesion and Migration Mediators Inflamm., 2020-04-07;2020(0):2713074. 2020-04-07 [PMID: 32322163] (Bioassay, Human) Bioassay Human
F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3) Int J Mol Sci, 2019-10-24;20(21):. 2019-10-24 [PMID: 31653015] (Bioassay, Human) Bioassay Human
NV Ayala-Nune, G Follain, F Delalande, A Hirschler, E Partiot, GL Hale, BC Bollweg, J Roels, M Chazal, F Bakoa, M Carocci, S Bourdoulou, O Faklaris, SR Zaki, A Eckly, B Uring-Lamb, F Doussau, S Cianferani, C Carapito, FMJ Jacobs, N Jouvenet, JG Goetz, R Gaudin Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells Nat Commun, 2019-09-27;10(1):4430. 2019-09-27 [PMID: 31562326] (Bioassay, Human) Bioassay Human
MJ Bertrand, M Abran, F Maafi, D Busseuil, N Merlet, T Mihalache-, P Geoffroy, PL Tardif, A Abulrob, M Arbabi-Gha, F Ni, M Sirois, PL L'Allier, É Rhéaume, F Lesage, JC Tardif In Vivo Near-Infrared Fluorescence Imaging of Atherosclerosis Using Local Delivery of Novel Targeted Molecular Probes Sci Rep, 2019-02-25;9(1):2670. 2019-02-25 [PMID: 30804367] (Affinity Purification, Human) Affinity Purification Human
D Wolf, N Anto-Miche, H Blankenbac, A Wiedemann, K Buscher, JD Hohmann, B Lim, M Bäuml, A Marki, M Mauler, D Duerschmie, Z Fan, H Winkels, D Sidler, P Diehl, DM Zajonc, I Hilgendorf, P Stachon, T Marchini, F Willecke, M Schell, B Sommer, C von Zur Mu, J Reinöhl, T Gerhardt, EF Plow, V Yakubenko, P Libby, C Bode, K Ley, K Peter, A Zirlik A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense Nat Commun, 2018-02-06;9(1):525. 2018-02-06 [PMID: 29410422] (Bioassay, Human) Bioassay Human
Show All 31 Publications.

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Bioinformatics

Gene Symbol ICAM1
Uniprot