Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF


1 μg/lane of Recombinant HumanICAM-1/CD54 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 110 kDa and 190 kDa, respectively.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to recombinant human ICAM Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >60% will adhere after PMA 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived human ICAM-1/CD54 protein
Human ICAM-1
Accession # CAA30051
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
76 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
110-125 kDa, reducing conditions
Read Publications using
720-IC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 12 weeks, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58


Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

  1. Pigott, R. and C. Power, 1993, The Adhesion Molecule Facts Book, pp. 74. Academic Press.

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Publications for ICAM-1/CD54 (720-IC)(42)

We have publications tested in 4 confirmed species: Human, Mouse, Bacteria - Staphylococcus aureus, Goat.

We have publications tested in 6 applications: Adhesion Assay, Bioassay, Flow Cytometry, Migration, Surface Plasmon Resonance, Western Blot.

Filter By Application
Adhesion Assay
Flow Cytometry
Surface Plasmon Resonance
Western Blot
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Filter By Species
Bacteria - Staphylococcus aureus
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using 720-IC Applications Species
H Sun, Z Fan, AR Gingras, MA Lopez-Rami, MH Ginsberg, K Ley Frontline Science: A flexible kink in the transmembrane domain impairs β2 integrin extension and cell arrest from rolling J. Leukoc. Biol., 2019;0(0):. 2019 [PMID: 31475386] (Bioassay, Human) Bioassay Human
LC Ristow, V Tran, KJ Schwartz, L Pankratz, A Mehle, JD Sauer, RA Welch The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity MBio, 2019;10(4):. 2019 [PMID: 31289186] (Western Blot, Human) Western Blot Human
O Theodoly, N Garcia-Sey, F Bedu, X Luo, S Gabriele, T Mignot, J Giermanska, JP Chapel, M Métivier, MP Valignat Live nanoscopic to mesoscopic topography reconstruction with an optical microscope for chemical and biological samples PLoS ONE, 2018;13(12):e0207881. 2018 [PMID: 30540777] (Bioassay, Human) Bioassay Human
G Saggu, K Okubo, Y Chen, R Vattepu, N Tsuboi, F Rosetti, X Cullere, N Washburn, S Tahir, AM Rosado, SM Holland, RM Anthony, M Sen, C Zhu, TN Mayadas Cis interaction between sialylated Fc?RIIA and the ?I-domain of Mac-1 limits antibody-mediated neutrophil recruitment Nat Commun, 2018;9(1):5058. 2018 [PMID: 30498196]
BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff Biomimetic post-capillary venule expansions for leukocyte adhesion studies Sci Rep, 2018;8(1):9328. 2018 [PMID: 29921896]
F Jahan, S Madhavan, T Rolova, L Viazmina, M Grönholm, CG Gahmberg Phosphorylation of the ?-chain in the integrin LFA-1 enables ?2-chain phosphorylation and ?-actinin binding required for cell adhesion J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29903913] (Bioassay) Bioassay
CH Huang, YJ Liao, TH Fan, TJ Chiou, YH Lin, YC Twu A Developed NK-92MI Cell Line with Siglec-7negPhenotype Exhibits High and Sustainable Cytotoxicity against Leukemia Cells Int J Mol Sci, 2018;19(4):. 2018 [PMID: 29617289] (Bioassay) Bioassay
R Houmadi, D Guipouy, J Rey-Barros, Z Vasconcelo, J Cornet, M Manghi, N Destainvil, S Valitutti, S Allart, L Dupré The Wiskott-Aldrich Syndrome Protein Contributes to the Assembly of the LFA-1 Nanocluster Belt at the Lytic Synapse Cell Rep, 2018;22(4):979-991. 2018 [PMID: 29386139] (Bioassay, Human) Bioassay Human
C Arbuckle, M Greenberg, A Bergh, R German, N Sirago, E Linstead T-Time: A data repository of T cell and calcium release-activated calcium channel activation imagery BMC Res Notes, 2017;10(1):408. 2017 [PMID: 28807036] (Bioassay, Human) Bioassay Human
Q Yuan, C Ren, W Xu, B Petri, J Zhang, Y Zhang, P Kubes, D Wu, W Tang PKN1 Directs Polarized RAB21 Vesicle Trafficking via RPH3A and Is Important for Neutrophil Adhesion and Ischemia-Reperfusion Injury Cell Rep, 2017;19(12):2586-2597. 2017 [PMID: 28636945] (Bioassay, Goat) Bioassay Goat
Show All 42 Publications.

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Gene Symbol ICAM1