Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF


1 μg/lane of Recombinant HumanICAM-1/CD54 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 110 kDa and 190 kDa, respectively.

Product Details

Reactivity HuSpecies Glossary
Applications BA

Order Details

Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to recombinant human ICAM Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >60% will adhere after PMA 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived
Human ICAM-1
Accession # CAA30051
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
76 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
110-125 kDa, reducing conditions
Read Publications using
720-IC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 12 weeks, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58


Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

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Publications for (31)

We have publications tested in 4 confirmed species: Human, Mouse, Bacteria - Staphylococcus aureus, Goat.

We have publications tested in 6 applications: Adhesion Assay, Bioassay, Cell Adhesion, Flow, Migration assay, Surface Plasmon Resonance.

Filter By Application
Adhesion Assay
Cell Adhesion
Migration assay
Surface Plasmon Resonance
All Applications
Filter By Species
Bacteria - Staphylococcus aureus
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using 720-IC Applications Species
C Arbuckle, M Greenberg, A Bergh, R German, N Sirago, E Linstead T-Time: A data repository of T cell and calcium release-activated calcium channel activation imagery BMC Res Notes, 2017;10(1):408. 2017 [PMID: 28807036] (Bioassay, Human) Bioassay Human
Q Yuan, C Ren, W Xu, B Petri, J Zhang, Y Zhang, P Kubes, D Wu, W Tang PKN1 Directs Polarized RAB21 Vesicle Trafficking via RPH3A and Is Important for Neutrophil Adhesion and Ischemia-Reperfusion Injury Cell Rep, 2017;19(12):2586-2597. 2017 [PMID: 28636945] (Bioassay, Goat) Bioassay Goat
T Schober, T Magg, M Laschinger, M Rohlfs, ND Linhares, J Puchalka, T Weisser, K Fehlner, J Mautner, C Walz, K Hussein, G Jaeger, B Kammer, I Schmid, M Bahia, SD Pena, U Behrends, BH Belohradsk, C Klein, F Hauck A human immunodeficiency syndrome caused by mutations in CARMIL2 Nat Commun, 2017;8(0):14209. 2017 [PMID: 28112205] (Migration assay, Human) Migration assay Human
Recruitment of calcineurin to the TCR positively regulates T cell activation Nat. Immunol, 2016;0(0):. 2016 [PMID: 27941787] (Bioassay, Human) Bioassay Human
Identification of pathogenic factors potentially involved in Staphylococcus aureus keratitis using proteomics Exp Eye Res, 2016;151(0):171-178. 2016 [PMID: 27590660] (Bioassay, Bacteria - Staphylococcus aureus) Bioassay Bacteria - Staphylococcus aureus
Calcium influx through CRAC channels controls actin organization and dynamics at the immune synapse Elife, 2016;5(0):. 2016 [PMID: 27440222] (Bioassay, Human) Bioassay Human
Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells MBio, 2016;7(4):. 2016 [PMID: 27406562] (Bioassay, Human) Bioassay Human
WNK1 kinase balances T cell adhesion versus migration in vivo Nat Immunol, 2016;0(0):. 2016 [PMID: 27400149] (Bioassay, Human) Bioassay Human
Seung Hee Jung ARAP, a Novel Adaptor Protein, Is Required for TCR Signaling and Integrin-Mediated Adhesion J Immunol, 2016;0(0):. 2016 [PMID: 27335501] (Bioassay, Human) Bioassay Human
Yago T, Tsukamoto H, Liu Z, Wang Y, Thompson L, McEver R Multi-Inhibitory Effects of A2A Adenosine Receptor Signaling on Neutrophil Adhesion Under Flow. J Immunol, 2015;195(8):3880-9. 2015 [PMID: 26355151] (Bioassay, Human) Bioassay Human
Show All 31 Publications.

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Gene Symbol ICAM1