Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

Images

 
1 μg/lane of Recombinant HumanICAM-1/CD54 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 110 kDa and 190 kDa, respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to recombinant human ICAM Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >60% will adhere after PMA 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived
Human ICAM-1
Gln28-Glu480
Accession # CAA30051
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ICAM1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
76 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110-125 kDa, reducing conditions
Publications
Read Publications using
720-IC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 12 weeks, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

Customers Who Viewed This Item Also Viewed...

NB200-610
Species: Hu, Ca, GP
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP
NB500-328
Species: Hu
Applications: Flow, Func, IP, CyTOF-ready
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-2700
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF1008
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
NB110-89474
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-22542
Species: Hu
Applications: WB, B/N, Flow, ICC/IF, IHC, IHC-Fr, IP
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, KO

Publications for (31)

We have publications tested in 4 confirmed species: Human, Mouse, Bacteria - Staphylococcus aureus, Goat.

We have publications tested in 6 applications: Adhesion Assay, Bioassay, Cell Adhesion, Flow, Migration assay, Surface Plasmon Resonance.


Filter By Application
Adhesion Assay
(1)
Bioassay
(25)
Cell Adhesion
(1)
Flow
(2)
Migration assay
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(28)
Mouse
(1)
Bacteria - Staphylococcus aureus
(1)
Goat
(1)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using 720-IC Applications Species
C Arbuckle, M Greenberg, A Bergh, R German, N Sirago, E Linstead T-Time: A data repository of T cell and calcium release-activated calcium channel activation imagery BMC Res Notes, 2017;10(1):408. 2017 [PMID: 28807036] (Bioassay, Human) Bioassay Human
Q Yuan, C Ren, W Xu, B Petri, J Zhang, Y Zhang, P Kubes, D Wu, W Tang PKN1 Directs Polarized RAB21 Vesicle Trafficking via RPH3A and Is Important for Neutrophil Adhesion and Ischemia-Reperfusion Injury Cell Rep, 2017;19(12):2586-2597. 2017 [PMID: 28636945] (Bioassay, Goat) Bioassay Goat
T Schober, T Magg, M Laschinger, M Rohlfs, ND Linhares, J Puchalka, T Weisser, K Fehlner, J Mautner, C Walz, K Hussein, G Jaeger, B Kammer, I Schmid, M Bahia, SD Pena, U Behrends, BH Belohradsk, C Klein, F Hauck A human immunodeficiency syndrome caused by mutations in CARMIL2 Nat Commun, 2017;8(0):14209. 2017 [PMID: 28112205] (Migration assay, Human) Migration assay Human
Recruitment of calcineurin to the TCR positively regulates T cell activation Nat. Immunol, 2016;0(0):. 2016 [PMID: 27941787] (Bioassay, Human) Bioassay Human
Identification of pathogenic factors potentially involved in Staphylococcus aureus keratitis using proteomics Exp Eye Res, 2016;151(0):171-178. 2016 [PMID: 27590660] (Bioassay, Bacteria - Staphylococcus aureus) Bioassay Bacteria - Staphylococcus aureus
Calcium influx through CRAC channels controls actin organization and dynamics at the immune synapse Elife, 2016;5(0):. 2016 [PMID: 27440222] (Bioassay, Human) Bioassay Human
Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells MBio, 2016;7(4):. 2016 [PMID: 27406562] (Bioassay, Human) Bioassay Human
WNK1 kinase balances T cell adhesion versus migration in vivo Nat Immunol, 2016;0(0):. 2016 [PMID: 27400149] (Bioassay, Human) Bioassay Human
Seung Hee Jung ARAP, a Novel Adaptor Protein, Is Required for TCR Signaling and Integrin-Mediated Adhesion J Immunol, 2016;0(0):. 2016 [PMID: 27335501] (Bioassay, Human) Bioassay Human
Yago T, Tsukamoto H, Liu Z, Wang Y, Thompson L, McEver R Multi-Inhibitory Effects of A2A Adenosine Receptor Signaling on Neutrophil Adhesion Under Flow. J Immunol, 2015;195(8):3880-9. 2015 [PMID: 26355151] (Bioassay, Human) Bioassay Human
Show All 31 Publications.

Reviews for ICAM-1/CD54 (0)

There are no reviews for ICAM-1/CD54. By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for ICAM-1/CD54 (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

CD11b Antibody
NB110-89474

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol ICAM1
Entrez
Uniprot