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Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

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1 μg/lane of Recombinant HumanICAM-1/CD54 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 110 kDa and 190 kDa, respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to recombinant human ICAM Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >60% will adhere after PMA 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ICAM-1/CD54 protein
Human ICAM-1
Gln28-Glu480
Accession # CAA30051
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ICAM1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
76 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110-125 kDa, reducing conditions
Publications
Read Publications using
720-IC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 12 weeks, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

  1. Pigott, R. and C. Power, 1993, The Adhesion Molecule Facts Book, pp. 74. Academic Press.

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Applications: Bioactivity

Publications for ICAM-1/CD54 (720-IC)(62)

We have publications tested in 4 confirmed species: Human, Mouse, Bacteria - Staphylococcus aureus, Goat.

We have publications tested in 8 applications: Adhesion Assay, Bioassay, Cell Culture, ELISA Capture Control, Flow Cytometry, Migration, Surface Plasmon Resonance, Western Blot.


Filter By Application
Adhesion Assay
(2)
Bioassay
(52)
Cell Culture
(2)
ELISA Capture Control
(1)
Flow Cytometry
(3)
Migration
(1)
Surface Plasmon Resonance
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(51)
Mouse
(4)
Bacteria - Staphylococcus aureus
(1)
Goat
(1)
All Species
Showing Publications 1 - 10 of 62. Show All 62 Publications.
Publications using 720-IC Applications Species
Wilkins, GC;Gilmour, J;Giannoudaki, E;Kirby, JA;Sheerin, NS;Ali, S; Dissecting the Therapeutic Mechanisms of Sphingosine-1-Phosphate Receptor Agonism during Ischaemia and Reperfusion International journal of molecular sciences 2023-07-07 [PMID: 37446370] (Bioassay, Human) Bioassay Human
Toffali, L;D'Ulivo, B;Giagulli, C;Montresor, A;Zenaro, E;Delledonne, M;Rossato, M;Iadarola, B;Sbarbati, A;Bernardi, P;Angelini, G;Rossi, B;Lopez, N;Linke, WA;Unger, A;Di Silvestre, D;Benazzi, L;De Palma, A;Motta, S;Constantin, G;Mauri, P;Laudanna, C; An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking Cell reports 2023-05-18 [PMID: 37204926] (Bioassay, Human) Bioassay Human
Gr�bnitz, F;Stark, D;Shlesinger, D;Petkidis, A;Borsa, M;Yermanos, A;Carr, A;Barandun, N;Wehling, A;Balaz, M;Schroeder, T;Oxenius, A; Asymmetric cell division safeguards memory CD8 T�cell development Cell reports 2023-05-12 [PMID: 37178119] (Bioassay, Mouse) Bioassay Mouse
I Rohwedder, LM Wackerbart, K Heinig, A Ballweg, J Altstätter, M Ripphahn, C Nussbaum, M Salvermose, S Bierschenk, T Straub, M Gunzer, M Schmidt-Su, T Kolben, C Schulz, A Ma, B Walzog, M Heinig, M Sperandio A20 and the non-canonical NF-kappaB pathway are key regulators of neutrophil recruitment during fetal ontogeny JCI Insight, 2023-02-22;0(0):. 2023-02-22 [PMID: 36633909] (Bioassay, Human) Bioassay Human
VA Morikis, SJ Chen, J Madigan, MH Jo, LC Werba, T Ha, SI Simon beta2-Integrin Adhesive Bond Tension under Shear Stress Modulates Cytosolic Calcium Flux and Neutrophil Inflammatory Response Cells, 2022-09-09;11(18):. 2022-09-09 [PMID: 36139397] (Bioassay, Human) Bioassay Human
L Wen, A Marki, Z Wang, M Orecchioni, J Makings, M Billitti, E Wang, SSA Suthahar, K Kim, WB Kiosses, Z Mikulski, K Ley A humanized beta2 integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in�vivo Cell Reports, 2022-05-31;39(9):110876. 2022-05-31 [PMID: 35649374] (Bioassay, Human) Bioassay Human
M Mastrogiov, P Vargas, T Rose, C Cuche, E Esposito, M Juzans, H Laude, A Schneider, M Bernard, S Goyard, C Renaudat, MN Ungeheuer, J Delon, A Alcover, V Di Bartolo The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration Science Advances, 2022-04-13;8(15):eabl5942. 2022-04-13 [PMID: 35417240] (Bioassay, Human) Bioassay Human
W Liu, AY Hsu, Y Wang, T Lin, H Sun, JS Pachter, A Groisman, M Imperioli, FW Yungher, L Hu, P Wang, Q Deng, Z Fan Mitofusin-2 regulates leukocyte adhesion and beta2 integrin activation Journal of leukocyte biology, 2021-09-08;0(0):. 2021-09-08 [PMID: 34494308] (Bioassay, Human) Bioassay Human
KP Eidell, A Lovy, NR Sylvain, FA Scangarell, HI Muendlein, MJ Ophir, K Nguyen, MC Seminario, SC Bunnell LFA-1 and Kindlin-3 enable the collaborative transport of SLP-76 microclusters by myosin and dynein motors Journal of Cell Science, 2021-08-27;0(0):. 2021-08-27 [PMID: 34279667] (Bioassay, Human) Bioassay Human
AL Lanz, G Masi, N Porciello, A Cohnen, D Cipria, D Prakaash, Š Bálint, R Raggiaschi, D Galgano, DK Cole, M Lepore, O Dushek, ML Dustin, MSP Sansom, AC Kalli, O Acuto Allosteric activation of T�cell antigen receptor signaling by quaternary structure relaxation Cell Reports, 2021-07-13;36(2):109375. 2021-07-13 [PMID: 34260912] (Bioassay, Human) Bioassay Human
Show All 62 Publications.

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Bioinformatics

Gene Symbol ICAM1
Uniprot