Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

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1 μg/lane of Recombinant HumanICAM-1/CD54 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 110 kDa and 190 kDa, respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to recombinant human ICAM Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >60% will adhere after PMA 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ICAM-1/CD54 protein
Human ICAM-1
Gln28-Glu480
Accession # CAA30051
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ICAM1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
76 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110-125 kDa, reducing conditions
Publications
Read Publications using
720-IC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 12 weeks, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

  1. Pigott, R. and C. Power, 1993, The Adhesion Molecule Facts Book, pp. 74. Academic Press.

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Publications for ICAM-1/CD54 (720-IC)(38)

We have publications tested in 4 confirmed species: Human, Mouse, Bacteria - Staphylococcus aureus, Goat.

We have publications tested in 6 applications: Adhesion Assay, Bioassay, Cell Adhesion, Flow, Migration assay, Surface Plasmon Resonance.


Filter By Application
Adhesion Assay
(1)
Bioassay
(31)
Cell Adhesion
(1)
Flow
(2)
Migration assay
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(31)
Mouse
(1)
Bacteria - Staphylococcus aureus
(1)
Goat
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using 720-IC Applications Species
BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff Biomimetic post-capillary venule expansions for leukocyte adhesion studies Sci Rep, 2018;8(1):9328. 2018 [PMID: 29921896]
F Jahan, S Madhavan, T Rolova, L Viazmina, M Grönholm, CG Gahmberg Phosphorylation of the ?-chain in the integrin LFA-1 enables ?2-chain phosphorylation and ?-actinin binding required for cell adhesion J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29903913] (Bioassay) Bioassay
CH Huang, YJ Liao, TH Fan, TJ Chiou, YH Lin, YC Twu A Developed NK-92MI Cell Line with Siglec-7negPhenotype Exhibits High and Sustainable Cytotoxicity against Leukemia Cells Int J Mol Sci, 2018;19(4):. 2018 [PMID: 29617289] (Bioassay) Bioassay
R Houmadi, D Guipouy, J Rey-Barros, Z Vasconcelo, J Cornet, M Manghi, N Destainvil, S Valitutti, S Allart, L Dupré The Wiskott-Aldrich Syndrome Protein Contributes to the Assembly of the LFA-1 Nanocluster Belt at the Lytic Synapse Cell Rep, 2018;22(4):979-991. 2018 [PMID: 29386139] (Bioassay, Human) Bioassay Human
C Arbuckle, M Greenberg, A Bergh, R German, N Sirago, E Linstead T-Time: A data repository of T cell and calcium release-activated calcium channel activation imagery BMC Res Notes, 2017;10(1):408. 2017 [PMID: 28807036] (Bioassay, Human) Bioassay Human
Q Yuan, C Ren, W Xu, B Petri, J Zhang, Y Zhang, P Kubes, D Wu, W Tang PKN1 Directs Polarized RAB21 Vesicle Trafficking via RPH3A and Is Important for Neutrophil Adhesion and Ischemia-Reperfusion Injury Cell Rep, 2017;19(12):2586-2597. 2017 [PMID: 28636945] (Bioassay, Goat) Bioassay Goat
V Nägele, A Kratzer, G Zugmaier, C Holland, Y Hijazi, MS Topp, N Gökbuget, PA Baeuerle, P Kufer, A Wolf, M Klinger Changes in clinical laboratory parameters and pharmacodynamic markers in response to blinatumomab treatment of patients with relapsed/refractory ALL Exp Hematol Oncol, 2017;6(0):14. 2017 [PMID: 28533941] (Bioassay, Human) Bioassay Human
EE Edwards, SN Thomas P-Selectin and ICAM-1 synergy in mediating THP-1 monocyte adhesion in hemodynamic flow is length dependent Integr Biol (Camb), 2017;9(4):313-327. 2017 [PMID: 28262902] (Bioassay, Human) Bioassay Human
T Schober, T Magg, M Laschinger, M Rohlfs, ND Linhares, J Puchalka, T Weisser, K Fehlner, J Mautner, C Walz, K Hussein, G Jaeger, B Kammer, I Schmid, M Bahia, SD Pena, U Behrends, BH Belohradsk, C Klein, F Hauck A human immunodeficiency syndrome caused by mutations in CARMIL2 Nat Commun, 2017;8(0):14209. 2017 [PMID: 28112205] (Migration assay, Human) Migration assay Human
Recruitment of calcineurin to the TCR positively regulates T cell activation Nat. Immunol, 2016;0(0):. 2016 [PMID: 27941787] (Bioassay, Human) Bioassay Human
Show All 38 Publications.

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Bioinformatics

Gene Symbol ICAM1
Uniprot