RNMT Antibody (3H3-1D12)

Images

 
Western Blot: RNMT Antibody (3H3-1D12) [H00008731-M01] - RNMT monoclonal antibody (M01), clone 3H3-1D12 Analysis of RNMT expression in HeLa.
Immunocytochemistry/ Immunofluorescence: RNMT Antibody (3H3-1D12) [H00008731-M01] - Analysis of monoclonal antibody to RNMT on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: RNMT Antibody (3H3-1D12) [H00008731-M01] - Analysis of monoclonal antibody to RNMT on formalin-fixed paraffin-embedded human squamous cell carcinoma tissue. Antibody concentration 3 ug/ml.
Sandwich ELISA: RNMT Antibody (3H3-1D12) [H00008731-M01] - Detection limit for recombinant GST tagged RNMT is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P
Clone
3H3-1D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RNMT Antibody (3H3-1D12) Summary

Immunogen
RNMT (AAH36798, 1 a.a. - 476 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANSAKAEEYEKMSLEQAKASVNSETESSFNINENTTASGTGLSEKTSVCRQVDIARKRKEFEDDLVKESSSCGKDTPSKKRKLDPEIVPEEKDCGDAEGNSKKRKRETEDVPKDKSSTGDGTQNKRKIALEDVPEKQKNLEEGHSSTVAAHYNELQEVGLEKRSQSRIFYLRNFNNWMKSVLIGEFLEKVRQKKKRDITVLDLGCGKGGDLLKWKKGRINKLVCTDIADVSVKQCQQRYEDMKNRRDSEYIFSAEFITADSSKELLIDKFRDPQMCFDICSCQFVCHYSFESYEQADMMLRNACERLSPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGDYPLFGCKYDFNLEGVVDVPEFLVYFPLLNEMAKKYNMKLVYKKTFLEFYEEKIKNNENKMLLKRMQALEPYPANESSKLVSEKVDDYEHAAKYMKNSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ
Localization
Nucleus
Specificity
RNMT - RNA (guanine-7-) methyltransferase
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RNMT
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNMT Antibody (3H3-1D12)

  • DKFZp686H1252
  • EC 2.1.1.56
  • hcm1p
  • hCMT1
  • hCMT1c
  • hMet
  • KIAA0398
  • mRNA (guanine-7-)methyltransferase
  • mRNA (guanine-N(7)-)-methyltransferase
  • mRNA cap guanine-N7 methyltransferase
  • mRNA cap methyltransferase
  • RG7MT1MET
  • RNA (guanine-7-) methyltransferase

Background

mRNA cap methyltransferase (HCM) catalyzes the N7 methylation of the 5' cap formed by mRNA capping enzyme (HCE) to form a terminal 7-methylguanosine. This is subsequently recognised by cap binding proteins that facilitate key events in gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RNMT
Entrez
OMIM
Uniprot