Recombinant Human HGF Protein, CF


Recombinant Human HGF (294-HG/CF) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to or less than 4.00 ng/mL.
Adult stem cell-derived liver organoids were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and liver organoid initiation medium, which includes Recombinant Human HGF (Catalog # 294-HG/CF), more

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations

Recombinant Human HGF Protein, CF Summary

Additional Information
An economically priced, mammalian-expressed Version Available!
Details of Functionality
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is equal to or less than 4.00 ng/mL.

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HGF protein
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession #
N-terminal Sequence
alpha chain: No results obtained; Gln32 predicted
beta chain: Val495
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
53.7 kDa ( alpha chain), 26 kDa ( beta chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
60-70 kDa, nonreducing conditions
60 kDa, and 33-34 kDa, reducing conditions
Read Publications using
294-HG/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 µg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HGF Protein, CF

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTA
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A


HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates human HGF isoforms that lack the proteinase‑like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH‑stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Publications for HGF (294-HG/CF)(108)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Equine, Fish - Prionace glauca (Blue Shark), Hamster, N/A, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Squirrel.

We have publications tested in 7 applications: Activation, Bioassay, Cell Culture, Control, ELISA (Standard), In Vivo, Proliferation.

Filter By Application
Cell Culture
ELISA (Standard)
In Vivo
All Applications
Filter By Species
Fish - Prionace glauca (Blue Shark)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
All Species
Showing Publications 1 - 10 of 108. Show All 108 Publications.
Publications using 294-HG/CF Applications Species
S Wenhua, T Tsunematsu, M Umeda, H Tawara, N Fujiwara, Y Mouri, R Arakaki, N Ishimaru, Y Kudo Cancer cell-derived novel periostin isoform promotes invasion in head and neck squamous cell carcinoma Cancer Medicine, 2023-01-23;0(0):. 2023-01-23 [PMID: 36691359] (Bioassay, Human) Bioassay Human
JE Kaserman, RB Werder, F Wang, T Matte, MI Higgins, M Dodge, J Lindstrom-, P Bawa, A Hinds, E Bullitt, IS Caballero, X Shi, RE Gerszten, N Brunetti-P, M Liesa, C Villacorta, AN Hollenberg, DN Kotton, AA Wilson Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity Cell Reports, 2022-12-06;41(10):111775. 2022-12-06 [PMID: 36476855] (Bioassay, Human) Bioassay Human
R Yoshida, M Saigi, T Tani, BF Springer, H Shibata, S Kitajima, NR Mahadevan, M Campisi, W Kim, Y Kobayashi, TC Thai, K Haratani, Y Yamamoto, SK Sundararam, EH Knelson, A Vajdi, I Canadas, R Uppaluri, CP Paweletz, JJ Miret, PH Lizotte, PC Gokhale, PA Janne, DA Barbie MET-induced CD73 restrains STING-mediated immunogenicity of EGFR-mutant lung cancer Cancer Research, 2022-11-02;0(0):. 2022-11-02 [PMID: 36066413] (Bioassay, Human) Bioassay Human
Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M Physical and functional interactome atlas of human receptor tyrosine kinases. EMBO Rep, 2022-04-05;23(6):e54041. 2022-04-05 [PMID: 35384245] (Bioassay, Human) Bioassay Human
J Krumm, K Sekine, P Samaras, A Brazovskaj, M Breunig, R Yasui, A Kleger, H Taniguchi, M Wilhelm, B Treutlein, JG Camp, B Kuster High temporal resolution proteome and phosphoproteome profiling of stem cell-derived hepatocyte development Cell Reports, 2022-03-29;38(13):110604. 2022-03-29 [PMID: 35354033] (Bioassay, Human) Bioassay Human
P Banerjee, GY Xiao, X Tan, VJ Zheng, L Shi, MNB Rabassedas, HF Guo, X Liu, J Yu, L Diao, J Wang, WK Russell, J Roszik, CJ Creighton, JM Kurie The EMT activator ZEB1 accelerates endosomal trafficking to establish a polarity axis in lung adenocarcinoma cells Nature Communications, 2021-11-03;12(1):6354. 2021-11-03 [PMID: 34732702] (Bioassay, Human) Bioassay Human
E Zahmatkesh, MH Ghanian, I Zarkesh, Z Farzaneh, M Halvaei, Z Heydari, F Moeinvazir, A Othman, M Ruo beta , A Piryaei, R Gramignoli, S Yakhkeshi, A Nüssler, M Najimi, H Baharvand, M Vosough Tissue-Specific Microparticles Improve Organoid Microenvironment for Efficient Maturation of Pluripotent Stem-Cell-Derived Hepatocytes Cells, 2021-05-21;10(6):. 2021-05-21 [PMID: 34063948] (Bioassay, Human) Bioassay Human
X Chamling, A Kallman, W Fang, CA Berlinicke, JL Mertz, P Devkota, IEM Pantoja, MD Smith, Z Ji, C Chang, A Kaushik, L Chen, KA Whartenby, PA Calabresi, HQ Mao, H Ji, TH Wang, DJ Zack Single-cell transcriptomic reveals molecular diversity and developmental heterogeneity of human stem cell-derived oligodendrocyte lineage cells Nature Communications, 2021-01-28;12(1):652. 2021-01-28 [PMID: 33510160] (Bioassay, Human) Bioassay Human
C Wang, M Zhang, G Garcia, E Tian, Q Cui, X Chen, G Sun, J Wang, V Arumugaswa, Y Shi ApoE-Isoform-Dependent SARS-CoV-2 Neurotropism and Cellular Response Cell Stem Cell, 2021-01-04;28(2):331-342.e5. 2021-01-04 [PMID: 33450186] (Bioassay, Human) Bioassay Human
L Gesualdi, E Leonetti, A Cucina, BM Scicchitan, S Sorrentino, MG Tarsitano, A Isidori, M Bizzarri, A Filippini, A Riccioli, M Cammarota, V Gigantino, G Ricci, A Catizone The PI3K/AKT Pathway Is Activated by HGF in NT2D1 Non-Seminoma Cells and Has a Role in the Modulation of Their Malignant Behavior Int J Mol Sci, 2020-11-17;21(22):. 2020-11-17 [PMID: 33212946] (Cell Culture, Human) Cell Culture Human
Show All 108 Publications.

Reviews for HGF (294-HG/CF) (0)

There are no reviews for HGF (294-HG/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for HGF (294-HG/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HGF Products

Blogs on HGF.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human HGF Protein, CF and receive a gift card or discount.


Gene Symbol HGF