HGF Proteins

294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED₅₀ for this effect is equal to less than 4.00 ng/mL." class="big_lightbox"> 294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED₅₀ for this effect is equal to less than 4.00 ng/mL." alt="Recombinant Human HGF (294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED₅₀ for this effect is equal to less than 4.00 ng/mL." class="big_thumb" />

354-BP), Recombinant Human EGF (236-EG), Recombinant Human FGF-10 (345-FG), Recombinant Human Noggin (6057-NG), Recombinant Human R-Spondin 1 (4645-RS), and Recombinant Human Wnt-3a (5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human HGF (Catalog # 294-HG), Recombinant Human BMP-7 (354-BP), Recombinant Human EGF (236-EG), and Recombinant Human FGF-19 (969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (AF2400; red) and counterstained with DAPI (5748; blue). " class="big_lightbox"> 354-BP), Recombinant Human EGF (236-EG), Recombinant Human FGF-10 (345-FG), Recombinant Human Noggin (6057-NG), Recombinant Human R-Spondin 1 (4645-RS), and Recombinant Human Wnt-3a (5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human HGF (Catalog # 294-HG), Recombinant Human BMP-7 (354-BP), Recombinant Human EGF (236-EG), and Recombinant Human FGF-19 (969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (AF2400; red) and counterstained with DAPI (5748; blue). " class="big_thumb" />

CP004) at 0.500 μg/mL (100 μL/well), Recombinant Human HGFR/c-MET Fc Chimera Protein (8614-MT) binds with an ED₅₀ of 6.00-60.0 ng/mL." class="big_lightbox"> CP004) at 0.500 μg/mL (100 μL/well), Recombinant Human HGFR/c-MET Fc Chimera Protein (8614-MT) binds with an ED₅₀ of 6.00-60.0 ng/mL." alt="Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human HGF Protein (Catalog # BT294) is captured on EvenCoat Streptavidin Coated Plates (CP004) at 0.500 μg/mL (100 μL/well), Recombinant Human HGFR/c-MET Fc Chimera Protein (8614-MT) binds with an ED₅₀ of 6.00-60.0 ng/mL." class="big_thumb" />