Recombinant Human GDNF Protein, CF


Recombinant Human GDNF (Catalog # 212‑GD/CF) induces SH-SY5Y human neuroblastoma cell proliferation in the presence of Recombinant Human GFR alpha ‑1 Fc Chimera (Catalog # 714‑GR). The activity is approximately more
1 μg/lane of Recombinant Human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 17 kDa and 34 kDa, respectively.

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Reactivity HuSpecies Glossary
Applications Binding Activity, Bioactivity

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Recombinant Human GDNF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714‑GR).
The specific activity of recombinant human GDNF is approximately 3.1 x 103 units/μg, which is calibrated against recombinant human GDNF Reference Standard (NIBSC code: 09/266). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) at 1 µg/mL can bind Recombinant Human GDNF with an apparent K<1 nM.
Mouse myeloma cell line, NS0-derived human GDNF protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
11.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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212-GD/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GDNF Protein, CF

  • Astrocyte-derived trophic factor
  • ATF
  • ATF1
  • ATF2
  • GDNF
  • glial cell derived neurotrophic factor
  • glial cell line derived neurotrophic factor
  • glial cell line-derived neurotrophic factor
  • HSCR3


Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

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Publications for GDNF (212-GD/CF)(107)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Bacteria, Chicken, Guinea Pig, Porcine, Rabbit, Synthetic.

We have publications tested in 6 applications: Bioassay, Cell Culture, Differentiation, ELISA (Capture), In Vivo, Surface Plasmon Resonance.

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Showing Publications 1 - 10 of 107. Show All 107 Publications.
Publications using 212-GD/CF Applications Species
SI Mae, M Ryosaka, S Sakamoto, K Matsuse, A Nozaki, M Igami, R Kabai, A Watanabe, K Osafune Expansion of Human iPSC-Derived Ureteric Bud Organoids with Repeated Branching Potential Cell Rep, 2020;32(4):107963. 2020 [PMID: 32726627] (Bioassay, Human) Bioassay Human
N Michel, P Narayanan, O Shomroni, M Schmidt Maturational Changes in Mouse Cutaneous Touch and Piezo2-Mediated Mechanotransduction Cell Rep, 2020;32(3):107912. 2020 [PMID: 32697985] (Bioassay, Mouse) Bioassay Mouse
H Tsujimoto, T Kasahara, SI Sueta, T Araoka, S Sakamoto, C Okada, SI Mae, T Nakajima, N Okamoto, D Taura, M Nasu, T Shimizu, M Ryosaka, Z Li, M Sone, M Ikeya, A Watanabe, K Osafune A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells Cell Rep, 2020;31(1):107476. 2020 [PMID: 32268094] (Bioassay, Human) Bioassay Human
P Kielkowski, IY Buchsbaum, VC Kirsch, NC Bach, M Drukker, S Cappello, SA Sieber FICD activity and AMPylation remodelling modulate human neurogenesis Nat Commun, 2020;11(1):517. 2020 [PMID: 31980631] (Differentiation, Human) Differentiation Human
AR Nickolls, MM Lee, DF Espinoza, M Szczot, RM Lam, Q Wang, J Beers, J Zou, MQ Nguyen, HJ Solinski, AA AlJanahi, KR Johnson, ME Ward, AT Chesler, CG Bönnemann Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells Cell Rep, 2020;30(3):932-946.e7. 2020 [PMID: 31968264] (Differentiation, Human) Differentiation Human
H Munezane, H Oizumi, T Wakabayash, S Nishio, T Hirasawa, T Sato, A Harada, T Yoshida, T Eguchi, Y Yamanashi, T Hashimoto, T Iwatsubo Roles of Collagen XXV and Its Putative Receptors PTPsigma/delta in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation Disorder Cell Rep, 2019;29(13):4362-4376.e6. 2019 [PMID: 31875546] (Cell Culture, Mouse) Cell Culture Mouse
A Bonafina, MF Trinchero, AS Ríos, P Bekinschte, AF Schinder, G Paratcha, F Ledda GDNF and GFRalpha1 Are Required for Proper Integration of Adult-Born Hippocampal Neurons Cell Rep, 2019;29(13):4308-4319.e4. 2019 [PMID: 31875542] (Cell Culture, Mouse) Cell Culture Mouse
M Chumarina, K Russ, C Azevedo, A Heuer, M Pihl, A Collin, EÅ Frostner, E Elmer, P Hyttel, G Cappellett, M Zini, S Goldwurm, L Roybon Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene Acta Neuropathol Commun, 2019;7(1):208. 2019 [PMID: 31843010] (Bioassay, Human) Bioassay Human
S Mahajani, A Raina, C Fokken, S Kügler, M Bähr Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors Cell Death Dis, 2019;10(12):898. 2019 [PMID: 31776327] (Cell Culture, Human) Cell Culture Human
T Hyvärinen, A Hyysalo, FE Kapucu, L Aarnos, A Vinogradov, SJ Eglen, L Ylä-Outine, S Narkilahti Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures Sci Rep, 2019;9(1):17125. 2019 [PMID: 31748598] (Differentiation, Human) Differentiation Human
Show All 107 Publications.

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Gene Symbol GDNF