Recombinant Human GDNF Protein, CF


1 ug/lane of Recombinant Human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 17 kDa and 34 kDa, respectively.
Recombinant Human GDNF (Catalog # 212‑GD/CF) induces SH-SY5Y human neuroblastoma cell proliferation in the presence of Recombinant Human GFR alpha ‑1 Fc Chimera (Catalog # 714‑GR). The ED50 for this effect more

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Reactivity HuSpecies Glossary
Applications BA, Bind

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Recombinant Human GDNF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714‑GR).
The specific activity of recombinant human GDNF is approximately 3.1 x 103 units/μg, which is calibrated against recombinant human GDNF Reference Standard (NIBSC code: 09/266). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) at 1 µg/mL can bind Recombinant Human GDNF with an apparent K<1 nM.
Mouse myeloma cell line, NS0-derived
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
11.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
212-GD/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GDNF Protein, CF

  • Astrocyte-derived trophic factor
  • ATF
  • ATF1
  • ATF2
  • GDNF
  • glial cell derived neurotrophic factor
  • glial cell line derived neurotrophic factor
  • glial cell line-derived neurotrophic factor
  • hGDNF
  • HSCR3


Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

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Publications for (50)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Bacteria, Chicken, Guinea Pig, Porcine, Rabbit, Synthetic.

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, ELISA capture, In Vivo, Surface Plasmon Resonance.

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Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using 212-GD/CF Applications Species
M Höllerhage, C Moebius, J Melms, WH Chiu, JN Goebel, T Chakroun, T Koeglsperg, WH Oertel, TW Rösler, M Bickle, GU Höglinger Protective efficacy of phosphodiesterase-1 inhibition against alpha-synuclein toxicity revealed by compound screening in LUHMES cells Sci Rep, 2017;7(1):11469. 2017 [PMID: 28904388] (Bioassay, Human) Bioassay Human
J Liu, D Shang, Y Xiao, P Zhong, H Cheng, R Zhou Isolation and characterization of string-forming female germline stem cells from ovaries of neonatal mice J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28827310] (Bioassay, Mouse) Bioassay Mouse
J Drouin-Oue, S Lau, PL Brattås, D Rylander O, K Pircs, DA Grassi, LM Collins, R Vuono, A Andersson, G Westergren, C Graff, L Minthon, H Toresson, RA Barker, J Jakobsson, M Parmar REST suppression mediates neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways EMBO Mol Med, 2017;9(8):1117-1131. 2017 [PMID: 28646119] (Bioassay, Human) Bioassay Human
S Menges, G Minakaki, PM Schaefer, H Meixner, I Prots, U Schlötzer-, K Friedland, B Winner, TF Outeiro, KF Winklhofer, CA von Arnim, W Xiang, J Winkler, J Klucken Alpha-synuclein prevents the formation of spherical mitochondria and apoptosis under oxidative stress Sci Rep, 2017;7(0):42942. 2017 [PMID: 28224980] (Bioassay, Human) Bioassay Human
SK Goparaju, K Kohda, K Ibata, A Soma, Y Nakatake, T Akiyama, S Wakabayash, M Matsushita, M Sakota, H Kimura, M Yuzaki, SB Ko, MS Ko Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors Sci Rep, 2017;7(0):42367. 2017 [PMID: 28205555] (Bioassay, Human) Bioassay Human
SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres Neurotoxic reactive astrocytes are induced by activated microglia Nature, 2017;541(7638):481-487. 2017 [PMID: 28099414] (Bioassay, Human) Bioassay Human
Z Zhao, Y Ma, Z Chen, Q Liu, Q Li, D Kong, K Yuan, L Hu, T Wang, X Chen, Y Peng, W Jiang, Y Yu, X Liu Effects of Feeder Cells on Dopaminergic Differentiation of Human Embryonic Stem Cells Front Cell Neurosci, 2016;10(0):291. 2016 [PMID: 28066186] (Bioassay, Human) Bioassay Human
Small molecules increase direct neural conversion of human fibroblasts Sci Rep, 2016;6(0):38290. 2016 [PMID: 27917895] (Bioassay, Human) Bioassay Human
Sustained synchronized neuronal network activity in a human astrocyte co-culture system Sci Rep, 2016;6(0):36529. 2016 [PMID: 27819315] (Bioassay, Human) Bioassay Human
MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells Sci Rep, 2016;6(0):32532. 2016 [PMID: 27608814] (Bioassay, Human) Bioassay Human
Show All 50 Publications.

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Gene Symbol GDNF