Recombinant Human GDNF Protein, CF


Recombinant Human GDNF (Catalog # 212‑GD/CF) induces SH-SY5Y human neuroblastoma cell proliferation in the presence of Recombinant Human GFR alpha ‑1 Fc Chimera (Catalog # 714‑GR). The activity is approximately more
1 μg/lane of Recombinant Human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 17 kDa and 34 kDa, respectively.

Product Details

Reactivity HuSpecies Glossary
Applications Binding Activity, Bioactivity

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Recombinant Human GDNF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714‑GR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) at 1 µg/mL can bind Recombinant Human GDNF with an apparent K<1 nM.
Mouse myeloma cell line, NS0-derived human GDNF protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
  • Bioactivity
Theoretical MW
11.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
212-GD/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GDNF Protein, CF

  • Astrocyte-derived trophic factor
  • ATF
  • ATF1
  • ATF2
  • GDNF
  • glial cell derived neurotrophic factor
  • glial cell line derived neurotrophic factor
  • glial cell line-derived neurotrophic factor
  • HSCR3


Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

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Publications for GDNF (212-GD/CF)(178)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Bacteria, Chicken, Goat, Guinea Pig, Porcine, Rabbit, Synthetic, Transgenic Human, Transgenic Mouse.

We have publications tested in 6 applications: Bioassay, Cell Culture, Differentiation, ELISA (Capture), In Vivo, Surface Plasmon Resonance.

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Showing Publications 1 - 10 of 178. Show All 178 Publications.
Publications using 212-GD/CF Applications Species
HW Ong, Y Liang, W Richardson, ER Lowry, CI Wells, X Chen, M Silvestre, K Dempster, JA Silvaroli, JL Smith, H Wichterle, NS Pabla, SK Ultanir, AN Bullock, DH Drewry, AD Axtman A Potent and Selective CDKL5/GSK3 Chemical Probe is Neuroprotective bioRxiv : the preprint server for biology, 2023;0(0):. 2023 [PMID: 36798313] (Bioassay, Human) Bioassay Human
GR Linares, Y Li, WH Chang, J Rubin-Sigl, S Mendonca, S Hong, Y Eoh, W Guo, YH Huang, J Chang, S Tu, N Dorjsuren, M Santana, ST Hung, J Yu, J Perez, M Chickering, TY Cheng, CC Huang, SJ Lee, HJ Deng, KT Bach, K Gray, V Subramanya, J Rosenfeld, SV Alworth, H Goodarzi, JK Ichida SYF2 suppression mitigates neurodegeneration in models of diverse forms of ALS Cell Stem Cell, 2023;30(2):171-187.e14. 2023 [PMID: 36736291] (Bioassay, Human) Bioassay Human
CB Pantazis, A Yang, E Lara, JA McDonough, C Blauwendra, L Peng, H Oguro, J Kanaujiya, J Zou, D Sebesta, G Pratt, E Cross, J Blockwick, P Buxton, L Kinner-Bib, C Medura, C Tompkins, S Hughes, M Santiana, F Faghri, MA Nalls, D Vitale, S Ballard, YA Qi, DM Ramos, KM Anderson, J Stadler, P Narayan, J Papademetr, L Reilly, MP Nelson, S Aggarwal, LU Rosen, P Kirwan, V Pisupati, SL Coon, SW Scholz, T Priebe, M Öttl, J Dong, M Meijer, LJM Janssen, VS Lourenco, R van der Ka, D Crusius, D Paquet, AC Raulin, G Bu, A Held, BJ Wainger, RMC Gabriele, JM Casey, S Wray, D Abu-Bonsra, CL Parish, MS Beccari, DW Cleveland, E Li, IVL Rose, M Kampmann, C Calatayud, P Verstreken, L Heinrich, MY Chen, B Schüle, D Dou, ELF Holzbaur, MC Zanellati, R Basundra, M Deshmukh, S Cohen, R Khanna, M Raman, ZS Nevin, M Matia, J Van Lent, V Timmerman, BR Conklin, K Johnson Ch, K Zhang, S Funes, DA Bosco, L Erlebach, M Welzer, D Kronenberg, G Lyu, E Arenas, E Coccia, L Sarrafha, T Ahfeldt, JC Marioni, WC Skarnes, MR Cookson, ME Ward, FT Merkle A reference human induced pluripotent stem cell line for large-scale collaborative studies Cell Stem Cell, 2022;29(12):1685-1702.e22. 2022 [PMID: 36459969] (Bioassay, Human) Bioassay Human
JR Herdy, L Traxler, RK Agarwal, L Karbacher, JCM Schlachetz, L Boehnke, D Zangwill, D Galasko, CK Glass, J Mertens, FH Gage Increased post-mitotic senescence in aged human neurons is a pathological feature of Alzheimer&#039;s disease Cell Stem Cell, 2022;29(12):1637-1652.e6. 2022 [PMID: 36459967] (Bioassay, Human) Bioassay Human
M Jones, A Cunningham, N Frank, D Sethi The monoculture of cord-blood-derived CD34+ cells by an automated, membrane-based dynamic perfusion system with a novel cytokine cocktail Stem Cell Reports, 2022;0(0):. 2022 [PMID: 36332632] (Bioassay, Human) Bioassay Human
G Kim, L Nakayama, JA Blum, T Akiyama, S Boeynaems, M Chakrabort, J Couthouis, E Tassoni-Ts, CM Rodriguez, MC Bassik, AD Gitler Genome-wide CRISPR screen reveals v-ATPase as a drug target to lower levels of ALS protein ataxin-2 Cell Reports, 2022;41(4):111508. 2022 [PMID: 36288714] (Bioassay, Human) Bioassay Human
C Seah, MS Breen, T Rusielewic, HN Bader, C Xu, CJ Hunter, B McCarthy, PJM Deans, M Chattopadh, J Goldberg, F Desarnaud, I Makotkine, JD Flory, LM Bierer, M Staniskyte, NYSCF Glob, SA Noggle, LM Huckins, D Paull, KJ Brennand, R Yehuda Modeling gene � environment interactions in PTSD using human neurons reveals diagnosis-specific glucocorticoid-induced gene expression Nature Neuroscience, 2022;25(11):1434-1445. 2022 [PMID: 36266471] (Bioassay, Human) Bioassay Human
T Patel, J Hammelman, S Aziz, S Jang, M Closser, TL Michaels, JA Blum, DK Gifford, H Wichterle Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro Nature Communications, 2022;13(1):5427. 2022 [PMID: 36109497] (Cell Culture, Mouse) Cell Culture Mouse
L Mahoney-Sa, H Bouchaoui, I Boussaad, A Jonneaux, K Timmerman, O Berdeaux, S Ayton, R Krüger, JA Duce, D Devos, JC Devedjian Alpha synuclein determines ferroptosis sensitivity in dopaminergic neurons via modulation of ether-phospholipid membrane composition Cell Reports, 2022;40(8):111231. 2022 [PMID: 36001957] (Bioassay, Human) Bioassay Human
R Lieberman, LK Cortes, G Gao, H Park, B Wang, PL Jones, RB Hunter, JP Leonard, RH Barker Human iPSC-derived astrocytes generated from donors with globoid cell leukodystrophy display phenotypes associated with disease PLoS ONE, 2022;17(8):e0271360. 2022 [PMID: 35921286] (Bioassay, Human) Bioassay Human
Show All 178 Publications.

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The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

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Gene Symbol GDNF