Recombinant Human Neurturin Protein, CF

Images

 
There are currently no images for Neurturin (1297-NE/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity, Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Neurturin Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 20-80 ng/mL in the presence of Recombinant Human GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 613‑FR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 613-FR) at 1 μg/mL can bind Recombinant Human Neurturin with an apparent KD <3.0 nM.
Source
E. coli-derived human Neurturin protein
Ala96-Val197, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NRTN
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
11.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1297-NE/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Neurturin Protein, CF

  • Neurturin
  • NRTN
  • NTN

Background

Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. GDNF family proteins are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1-4). Similarly to other TGF-beta family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active human Neurturin is a disulfide-linked homodimer of the carboxy-terminal 102 amino acid residues. Mature human Neurturin shares approximately 92% amino acid sequence identity with mouse Neurturin. Mature Neurturin also shares about 40% similarities with the other three members of the GDNF family ligands (2-5). Unlike other members of TGF-beta family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFR alpha -1-GFR alpha -4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFR alpha -2 but can also bind GFR alpha -1 at higher concentrations (5‑8). Neurturin had been shown to promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. Neurturin is expressed in both neuronal and non-neuronal tissues. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems and as well as non-neuronal systems (9).

  1. Lin, L-F.H. et al. (1993) Science 260:1130.
  2. Kotzbauer, P.T. et al. (1996) Nature 384:467.
  3. Milbrandt, J. et al. (1998) Neuron 20:245.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.
  5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
  6. Baloh, R.H. et al. (1997) Neuron 18:793.
  7. Jing, S. et al. (1996) Cell 85:1113.
  8. Jing, S. et al. (1997) J. Biol. Chem. 272:33111.
  9. Airaksinen. M. and M. Saarma (2002) Nature Review Neuroscience 3:383.

Customers Who Viewed This Item Also Viewed...

AF482
Species: Mu
Applications: WB, IHC
AF1085
Species: Mu
Applications: WB, IHC, Neut
AF429
Species: Hu, Mu
Applications: WB, IHC, Block, ICC
MAB2388
Species: Hu
Applications: WB, IHC
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, ICC, IHC-FrFl, IHC-WhMt
NBP2-57362
Species: Hu
Applications: WB, ICC/IF
AF2645
Species: Mu
Applications: WB, IHC
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24557
Species: Hu, Eq, Pm
Applications: WB, IHC-P
NLS54
Species: Hu, Mu, Ca
Applications: WB, ICC/IF, IHC-P, ICC
NBP2-02450
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82452
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF5659
Species: Hu
Applications: WB
1297-NE/CF
Species: Hu
Applications: Binding Activity, Bioactivity

Publications for Neurturin (1297-NE/CF)(6)

We have publications tested in 4 confirmed species: Human, Rat, Bacteria, Guinea Pig.

We have publications tested in 4 applications: Bioassay, ELISA (Capture), In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(3)
ELISA (Capture)
(1)
In Vivo
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(2)
Rat
(1)
Bacteria
(1)
Guinea Pig
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using 1297-NE/CF Applications Species
JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction Mol Ther Methods Clin Dev, 2018;9(0):172-180. 2018 [PMID: 29560384] (Bioassay, Human) Bioassay Human
Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011;286(34):30034-46. 2011 [PMID: 21737454] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain. Neuropharmacology, 2010;58(7):1114-21. 2010 [PMID: 20153340] (In Vivo, Rat) In Vivo Rat
Lu B, PereiraPerrin M A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008;76(8):3530-8. 2008 [PMID: 18541656] (ELISA (Capture), Bacteria) ELISA (Capture) Bacteria
Girard BM, Young BA, Buttolph TR, White SL, Parsons RL Regulation of neuronal pituitary adenylate cyclase-activating polypeptide expression during culture of guinea-pig cardiac ganglia. Neuroscience, 2007;146(2):584-93. 2007 [PMID: 17367946] (Bioassay, Guinea Pig) Bioassay Guinea Pig
Girard BM, Young BA, Buttolph TR, Locknar SA, White SL, Parsons RL Trophic factor modulation of cocaine- and amphetamine-regulated transcript peptide expression in explant cultured guinea-pig cardiac neurons. Neuroscience, 2006;139(4):1329-41. 2006 [PMID: 16516394] (Bioassay, Guinea Pig) Bioassay Guinea Pig

Reviews for Neurturin (1297-NE/CF) (0)

There are no reviews for Neurturin (1297-NE/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Neurturin (1297-NE/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Neurturin Products

Bioinformatics Tool for Neurturin (1297-NE/CF)

Discover related pathways, diseases and genes to Neurturin (1297-NE/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Neurturin (1297-NE/CF)

Discover more about diseases related to Neurturin (1297-NE/CF).
 

Pathways for Neurturin (1297-NE/CF)

View related products by pathway.

PTMs for Neurturin (1297-NE/CF)

Learn more about PTMs related to Neurturin (1297-NE/CF).

Blogs on Neurturin

There are no specific blogs for Neurturin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Neurturin Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol NRTN
Uniprot