GDNF Antibodies

BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="GDNF and GFRa-1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Biotinylated Goat Anti-Rat GFRa-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-212-NA). The ND₅₀ is typically 0.25-1 µg/mL." class="big_lightbox"> 212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-212-NA). The ND₅₀ is typically 0.25-1 µg/mL." class="big_thumb" />

212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by GDNF (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AB-212-NA). The ND₅₀is typically 7.5-30 µg/mL." class="big_lightbox"> 212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by GDNF (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AB-212-NA). The ND₅₀is typically 7.5-30 µg/mL." alt="Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by GDNF (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AB-212-NA). The ND₅₀is typically 7.5-30 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="GDNF was detected in immersion fixed WM‑115 Human Malignant Melanoma Cell Line (positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (negative) Cells using Mouse Anti-Human GDNF Monoclonal Antibody (Catalog # MAB2121) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB2122) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF512) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB2122) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF512) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human GDNF protein was serially diluted 2-fold and captured by Mouse Anti-Human GDNF Monoclonal Antibody (MAB2122) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF512) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Goat Anti-Human GDNF Antigen Affinity-purified Polyclonal Antibody (AF512) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Goat Anti-Human GDNF Antigen Affinity-purified Polyclonal Antibody (AF512) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human GDNF protein was serially diluted 2-fold and captured by Mouse Anti-Human GDNF Monoclonal Antibody (Catalog # MAB2122) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human GDNF Antigen Affinity-purified Polyclonal Antibody (AF512) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />