GDNF Antibody

Images

 
GDNF and GFR alpha ‑1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and ...read more
Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH‑SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) in a ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GDNF Antibody Summary

Immunogen
E. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF
Arg109-Ile211
Accession # P39905
Specificity
Detects human and rat GDNF in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GDNF
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.25-1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.3
using
AF-212-NA in the following applications:

Publications
Read Publications using
AF-212-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDNF Antibody

  • Astrocyte-derived trophic factor
  • ATF
  • ATF1
  • ATF2
  • GDNF
  • glial cell derived neurotrophic factor
  • glial cell line derived neurotrophic factor
  • glial cell line-derived neurotrophic factor
  • HFB1-GDNF
  • HGDNF
  • HSCR3

Background

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GDNF Antibody (AF-212-NA)(29)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 8 applications: Affinity Assay, ELISA Development (Detection), ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Affinity Assay
(1)
ELISA Development (Detection)
(1)
ICC
(2)
IHC
(9)
IHC-Fr
(7)
IHC-P
(2)
Neutralization
(4)
Western Blot
(3)
All Applications
Filter By Species
Human
(6)
Mouse
(5)
Rat
(11)
Chicken
(1)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(4)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using AF-212-NA Applications Species
L Quintino, A Namislo, M Davidsson, LS Breger, P Kavanagh, M Avallone, E Elgstrand-, C Isaksson, C Lundberg Destabilizing Domains Enable Long-Term and Inert Regulation of GDNF Expression in the Brain Mol Ther Methods Clin Dev, 2018;11(0):29-39. 2018 [PMID: 30324128] (IHC, Rat) IHC Rat
R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29339495] (Affinity Assay) Affinity Assay
K Nagashima, T Miwa, H Soumiya, D Ushiro, T Takeda-Kaw, N Tamaoki, S Ishiguro, Y Sato, K Miyamoto, T Ohno, M Osawa, T Kunisada, T Shibata, KI Tezuka, S Furukawa, H Fukumitsu Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury Sci Rep, 2017;7(1):13500. 2017 [PMID: 29044129] (ICC, Human) ICC Human
J Lavaur, D Le Nogue, M Lemaire, J Pype, G Farjot, EC Hirsch, PP Michel The Noble Gas Xenon Provides Protection and Trophic Stimulation to Midbrain Dopamine Neurons J. Neurochem., 2017;0(0):. 2017 [PMID: 28398653] (Neutralization, Rat) Neutralization Rat
Y Dong, A Kanda, K Noda, W Saito, S Ishida Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane Sci Rep, 2017;7(0):44266. 2017 [PMID: 28276504] (IHC-P, Human) IHC-P Human
GM Thomsen, M Alkaslasi, JP Vit, G Lawless, M Godoy, G Gowing, O Shelest, CN Svendsen Systemic injection of AAV9-GDNF provides modest functional improvements in the SOD1(G93A) ALS rat but has adverse side effects Gene Ther, 2017;0(0):. 2017 [PMID: 28276446] (IHC, Rat) IHC Rat
SR Stott, S Hayat, T Carnwath, S Garas, JP Sleeman, RA Barker CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson&#039;s disease PLoS ONE, 2017;12(2):e0171748. 2017 [PMID: 28182766] (IHC, Mouse) IHC Mouse
Wang T, Bruggeman K, Sheean R, Turner B, Nisbet D, Parish C Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair. J Biol Chem, 2014;289(21):15044-51. 2014 [PMID: 24700461] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Sarin S, Boivin F, Li A, Lim J, Svajger B, Rosenblum N, Bridgewater D beta-Catenin overexpression in the metanephric mesenchyme leads to renal dysplasia genesis via cell-autonomous and non-cell-autonomous mechanisms. Am J Pathol, 2014;184(5):1395-410. 2014 [PMID: 24637293] (IHC-P, Mouse) IHC-P Mouse
Quintino L, Manfre G, Wettergren E, Namislo A, Isaksson C, Lundberg C Functional neuroprotection and efficient regulation of GDNF using destabilizing domains in a rodent model of Parkinson&#039;s disease. Mol Ther, 2013;21(12):2169-80. 2013 [PMID: 23881415] (Western Blot, Human) Western Blot Human
Show All 29 Publications.

Reviews for GDNF Antibody (AF-212-NA) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using AF-212-NA:
Filter by Applications
IF
(1)
IP
(1)
WB
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence GDNF AF-212-NA
Enlarge
5
reviewed by:
Anonymous
IF Human 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHuman GDNF bound to the surface of HEK293 cells transfected with the GDNF receptor GFRa1
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
  4
reviewed by:
Anonymous
IP Human 12/18/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedN/A
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/
  4
reviewed by:
Anonymous
WB Human 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedRecombinant GDNF binding to 293 cells expressing GDNF receptors or primary hippocampal neurons or brain homogenates
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GDNF Antibody (AF-212-NA) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF212

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Bioinformatics Tool for GDNF Antibody (AF-212-NA)

Discover related pathways, diseases and genes to GDNF Antibody (AF-212-NA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on GDNF.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
12/18/2014
Application: IF
Species: Human

 
Anonymous
12/18/2014
Application: IP
Species: Human

 
Anonymous
12/18/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol GDNF
Uniprot