Recombinant Human FGF-4, Animal-Free Protein

Recombinant HumanFGF-4, Animal-Free activity is determined using the firefly luciferase reporter assay (*) in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-4. Firefly luciferase activity is measured and normalized. EC50 = 4.3 ng/mL (306.5 pM). *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Recombinant human FGF-4 (Catalog # Qk004) migrates as a single band at 14 kDa in non-reducing conditions (NR) and upon reduction (R). No contaminating protein bands are visible.Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta -mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human FGF-4, Animal-Free Protein Summary

• Details of Functionality: No significant difference between EC 50 of reference and test lots
• Source: E. coli-derived human FGF-4 protein
• Accession #: P08620.1
• Protein/Peptide Type: Animal-Free Recombinant Proteins
• Purity: Single species with expected mass
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: Monomeric FGF-4 protein only

Packaging, Storage & Formulations

• Storage: Store lyophilized protein between -20 degreesC and -80 degreesC until the date of expiry. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from HEPES/NaCl/mannitol
• Purity: Single species with expected mass
• Reconstitution Instructions: Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired.

Notes

The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use. This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-4, Animal-Free Protein

• FGF4
• FGF-4
• fibroblast growth factor 4
• HBGF-4
• HBGF-4Transforming protein KS3
• heparin secretory transforming protein 1
• Heparin secretory-transforming protein 1
• Heparin-binding growth factor 4
• HST-1
• HST-1HSTF-1
• HSTF1fibroblast growth factor 4 splice isoform
• HSTFGF-4
• human stomach cancer, transforming factor from FGF-related oncogene
• kaposi sarcoma oncogene
• KFGF
• K-FGF
• KS3
• oncogene HST

Background

FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The human FGF-4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature human FGF-4 (aa 71-206) shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF-4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the mouse trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3-5). Later in mouse development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6-9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10-12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
3. Niswander, L. and G.R. Martin (1992) Development 114:755.
4. Feldman, B. et al. (1995) Science 267:246.
5. Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
6. Sun, X. et al. (2002) Nature 418:501.
7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
8. Yu, K and D.M. Ornitz (2008) Development 135:483.
9. Mariani, F.V. et al. (2008) Nature 453:401.
10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
11. Kunath, T. et al. (2007) Development 134:2895.
12. Mayshar, Y. et al. (2008) Stem Cells 26:767.
13. Hajitou, A. et al. (1998) Oncogene 17:2059.
14. Flynn, A. and T. O’Brien (2008) IDrugs 11:283.

Bioinformatics

• Uniprot:
  • P08620.1()