251-KG), Recombinant Human Noggin (
6057-NG), and Recombinant Human R-Spondin 1 (
4645-RS), along with the other reagents listed in the lung organoid expansion medium recipe in the
lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog # NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (
AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (
AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (
MAB2018; red). All samples were counterstained with DAPI (
5748; blue). " class="big_lightbox">
251-KG), Recombinant Human Noggin (
6057-NG), and Recombinant Human R-Spondin 1 (
4645-RS), along with the other reagents listed in the lung organoid expansion medium recipe in the
lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog # NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (
AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (
AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (
MAB2018; red). All samples were counterstained with DAPI (
5748; blue). " class="big_thumb" />
251-KG), Recombinant Human Noggin (
![SDS-Page: Human FGF-10 Protein [NBP2-34927]](http://images.novusbio.com/fullsize/Human-FGF-10-Protein-SDS-Page-NBP2-34927-img0001.jpg "SDS-Page: Human FGF-10 Protein [NBP2-34927]")
![ELISA: Human FGF-10 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31612] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31612_human-fgf-10-ready-to-use-elisa-kit-colorimetric-206202415301578.png "ELISA: Human FGF-10 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31612] - Standard Curve Reference")
![Western Blot: FGF10 Overexpression Lysate (Adult Normal) [NBP2-07870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGF10.](http://images.novusbio.com/fullsize/FGF10-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07870-img0001.jpg "Western Blot: FGF10 Overexpression Lysate (Adult Normal) [NBP2-07870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGF10.")
![SDS-Page: Rat FGF-10 Protein [NBP2-35261]](http://images.novusbio.com/fullsize/Rat-FGF-10-Protein-SDS-Page-NBP2-35261-img0001.jpg "SDS-Page: Rat FGF-10 Protein [NBP2-35261]")
![Western Blot: FGF-10 Antibody [NBP2-99399] - Anti-FGF-10 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 18 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/FGF-10-Antibody-Western-Blot-NBP2-99399-img0002.jpg "Western Blot: FGF-10 Antibody [NBP2-99399] - Anti-FGF-10 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 18 kDa (We are unsure as to the identity of these extra bands).")
![Proximity Ligation Assay: FGF-10 Antibody (3C7) [H00002255-M05] - Analysis of protein-protein interactions between FGFR2 and FGF10. HeLa cells were stained with anti-FGFR2 rabbit purified polyclonal 1:1200 and anti-FGF10 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction](http://images.novusbio.com/fullsize/FGF-10-Antibody-3C7-Proximity-Ligation-Assay-H00002255-M05-img0001.jpg "Proximity Ligation Assay: FGF-10 Antibody (3C7) [H00002255-M05] - Analysis of protein-protein interactions between FGFR2 and FGF10. HeLa cells were stained with anti-FGFR2 rabbit purified polyclonal 1:1200 and anti-FGF10 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction")
