FGF-10 Products

HAF016). A specific band was detected for FGF-10 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox" target="_blank">

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">

251-KG), Recombinant Human Noggin (6057-NG), and Recombinant Human R-Spondin 1 (4645-RS), along with the other reagents listed in the lung organoid expansion medium recipe in the lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog # NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (MAB2018; red). All samples were counterstained with DAPI (5748; blue). " class="big_lightbox" target="_blank">

345-FG) were either untreated or incubated at 37°C for 9 days in media. The heat-stable (HS) FGF‑10 retained similar bioactivity after incubation compared to the untreated HS protein, indicating that the HS protein has increased thermal stability. In contrast, the wild-type (WT) FGF‑10 protein showed a significant loss of activity following incubation, suggesting less thermal stability." class="big_lightbox" target="_blank">

345-FG) and Heat Stable FGF-10HS (BT-FGF10HS) were incubated in DMEM media at 37°C for 48 or 72 hrs at concentrations of 600 ng/mL. Specific activity was assessed through a SEAP bioassay in HEK293 cells, wherein cells were treated with FGF-10 WT or FGF-10HS for 24 hours. The specific activity % of control was determined, with the control comprising FGF-10 stored at 4°C without any incubation. " class="big_lightbox" target="_blank">

665-FR) was captured on Biacore Sensor Chip CM5 via Recombinant Protein A/G/L (Catalog # NBP2-34985), and binding to Recombinant Human FGF 10 Heat Stable (Catalog # BT-FGF10HS) was measured at a concentration range between 0.0488 nM and 12.5 nM. The double-referenced sensorgram was fit to a two-state reaction binding model to determine the binding kinetics and affinity, with an affinity constant of KD=36.5 pM. (Biacore T200)." class="big_lightbox" target="_blank">

665-FR) was captured on Biacore Sensor Chip CM5 via Recombinant Protein A/G/L (Catalog # NBP2-34985), and binding to Recombinant Human FGF 10 (Catalog # 345-FG) was measured at a concentration range between 0.0488 nM and 12.5 nM. The double-referenced sensorgram was fit to a two-state reaction binding model to determine the binding kinetics and affinity, with an affinity constant of KD=36.2 pM. (Biacore T200)." class="big_lightbox" target="_blank">

345-FG). FGF-10 Heat Stable demonstrates that it can support organoid growth as effectively as wild type in an extracellular matrix. Scale bar indicates 1 mm." class="big_lightbox" target="_blank">

CSH-RUO-01) with either Recombinant Human FGF-10 Heat Stable Protein (HS, Catalog # BT-FGF10HS) or wild type Recombinant Human FGF-10 Protein (WT, Catalog # 345-FG). FGF-10 Heat Stable Protein demonstrates that it can support organoid growth as effectively as wild type in a defined animal-free extracellular matrix. Scale bar indicates 1 mm." class="big_lightbox" target="_blank">