SPRY2 Antibody (1E10)

Images

 
Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - SPRY2 monoclonal antibody (M01), clone 1E10 Analysis of SPRY2 expression in C32.
Immunocytochemistry/ Immunofluorescence: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on HeLa cell. Antibody concentration 25 ug/ml.
Immunohistochemistry-Paraffin: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 3 ug/ml.
Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody (M01), clone 1E10.Lane 1: SPRY2 transfected lysate(34.7 KDa).Lane 2: ...read more
ELISA: SPRY2 Antibody (1e10) [H00010253-M01] - Detection limit for recombinant GST tagged SPRY2 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
1E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SPRY2 Antibody (1E10) Summary

Immunogen
SPRY2 (AAH15745.1, 1 a.a. ~ 315 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEARAQSGNGSQPLLQTPRDGGRQRGEPDPRDALTQQVHVLSLDQIRAIRNTNEYTEGPTVVPRPGLKPAPRPSTQHKHERLHGLPEHRQPPRLQHSQVHSSARAPLSRSISTVSSGSRSSTRTSTSSSSSEQRLLGSSFSSGPVADGIIRVQPKSELKPGELKPLSKEDLGLHAYRCEDCGKCKCKECTYPRPLPSDWICDKQCLCSAQNVIDYGTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT
Localization
Cytoplasm. Cell membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).
Specificity
SPRY2 - sprouty homolog 2 (Drosophila)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SPRY2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publications using H00010253-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPRY2 Antibody (1E10)

  • hSPRY2
  • MGC23039
  • protein sprouty homolog 2
  • sprouty (Drosophila) homolog 2
  • sprouty 2
  • sprouty homolog 2 (Drosophila)
  • SPRY2
  • spry-2

Background

This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPRY2 Antibody (H00010253-M01)(3)

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Video Protocols

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Bioinformatics

Gene Symbol SPRY2
Entrez
OMIM
Uniprot