SPRY2 Antibody (1E10) - Azide and BSA Free

Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - SPRY2 monoclonal antibody (M01), clone 1E10 Analysis of SPRY2 expression in C32.

Immunocytochemistry/Immunofluorescence: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on HeLa cell. Antibody concentration 25 ug/ml.

Immunohistochemistry-Paraffin: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 3 ug/ml.

Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody (M01), clone 1E10.Lane 1: SPRY2 transfected lysate(34.7 KDa).Lane 2: Non-transfected lysate.

ELISA: SPRY2 Antibody (1e10) [H00010253-M01] - Detection limit for recombinant GST tagged SPRY2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SPRY2 Antibody (1E10) - Azide and BSA Free Summary

• Immunogen: SPRY2 (AAH15745.1, 1 a.a. ~ 315 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEARAQSGNGSQPLLQTPRDGGRQRGEPDPRDALTQQVHVLSLDQIRAIRNTNEYTEGPTVVPRPGLKPAPRPSTQHKHERLHGLPEHRQPPRLQHSQVHSSARAPLSRSISTVSSGSRSSTRTSTSSSSSEQRLLGSSFSSGPVADGIIRVQPKSELKPGELKPLSKEDLGLHAYRCEDCGKCKCKECTYPRPLPSDWICDKQCLCSAQNVIDYGTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT
• Localization: Cytoplasm. Cell membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).
• Specificity: SPRY2 - sprouty homolog 2 (Drosophila)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPRY2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPRY2 Antibody (1E10) - Azide and BSA Free

• hSPRY2
• MGC23039
• protein sprouty homolog 2
• sprouty (Drosophila) homolog 2
• sprouty 2
• sprouty homolog 2 (Drosophila)
• SPRY2
• Spry-2

Background

This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPRY2 Antibody (H00010253-M01)(4)

Publications using H00010253-M01

• Lee CT, Chu CA, Wang YM et al. Dual role of sprouty2 as an inhibitor of RAS/ERK-driven proliferation and a promoter of cancer invasion in KRAS wild-type colorectal cancer Molecular carcinogenesis 2023-04-04 [PMID: 37014154] (Western Blot)
• Ordonez-Moran P, Irmisch A, Barbachano A et al. SPROUTY2 is a b-catenin and FOXO3a target gene indicative of poor prognosis in colon cancer. Oncogene. 2013-04-29 [PMID: 23624922]
• Moghaddam SM, Amini A, Wei AQ et al. Initial Report on Differential Expression of Sprouty Proteins 1 and 2 in Human Epithelial Ovarian Cancer Cell Lines. J Oncol. 2012-11-29 [PMID: 23251157]
• Barbachano A, Ordonez-Moran P et al. SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity. Oncogene. 2010-06-14 [PMID: 20543868]

Bioinformatics

• Gene Symbol: SPRY2
• Entrez:
  • 10253(Human)
• OMIM:
  • 602466(Human)
• Uniprot:
  • AAH15745(Human)