Recombinant Human FGF-10 Protein, CF

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1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.
Recombinant Human FGF-10 (Catalog # 345-FG/CF) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50 for this effect is 20‑100 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human FGF-10 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 20-100 ng/mL.
Source
E. coli-derived human FGF-10 protein
Cys37-Ser208 & Gly41-Ser208
Accession #
N-terminal Sequence
Cys37 & Gly41
Protein/Peptide Type
Recombinant Proteins
Gene
FGF10
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
19-22 kDa, reducing conditions
Publications
Read Publications using
345-FG/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-10 Protein, CF

  • FGF10
  • FGF-10
  • fibroblast growth factor 10
  • Keratinocyte growth factor 2
  • KGF-2
  • produced by fibroblasts of urinary bladder lamina propria

Background

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (8, 9), formation of the otic vesicle and chochlea (10), adipogenesis (11), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (12 - 15). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 10, 13 ‑ 15). The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung (8, 9). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF‑10 biology (7, 10, 14, 15). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (16).

  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Igarashi, M. et al. (1998) J. Biol. Chem. 273:13230.
  3. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
  4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Min, H. et al. (1998) Genes Dev. 12:3156.
  7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
  8. Bellusci, S. et al. (1997) Development 124:4867.
  9. Weaver, M. et al. (2000) Development 127:2695.
  10. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
  11. Sakaue, H. et al. (2002) Genes Dev. 16:908.
  12. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
  13. Mailleux, A.A. et al. (2002) Development 129:53.
  14. Makarenkova, H.P. et al. (2000) Development 127:2563.
  15. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
  16. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

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Publications for FGF-10 (345-FG/CF)(44)

We have publications tested in 5 confirmed species: Human, Mouse, Canine, Xenopus, Zebrafish.

We have publications tested in 4 applications: Binding Assay, Bioassay, In Vivo, cell culture.


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Binding Assay
(1)
Bioassay
(40)
In Vivo
(2)
cell culture
(1)
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Human
(29)
Mouse
(12)
Canine
(1)
Xenopus
(1)
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Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using 345-FG/CF Applications Species
S Zhang, C Zhao, S Liu, Y Wang, Y Zhao, W Guan, Z Zhu Characteristics and multi?lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff Mol Med Rep, 2018;0(0):. 2018 [PMID: 29916546] (Bioassay, Canine) Bioassay Canine
H Lahlou, A Lopez-Juar, A Fontbonne, E Nivet, A Zine Modeling human early otic sensory cell development with induced pluripotent stem cells PLoS ONE, 2018;13(6):e0198954. 2018 [PMID: 29902227] (Bioassay, Human) Bioassay Human
X Guo, Y Xu, Z Wang, Y Wu, J Chen, G Wang, C Lu, W Jia, J Xi, S Zhu, Z Jiapaer, X Wan, Z Liu, S Gao, J Kang A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis Cell Stem Cell, 2018;0(0):. 2018 [PMID: 29754779] (Bioassay, Mouse) Bioassay Mouse
B Memon, M Karam, S Al-Khawaga, EM Abdelalim Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1 Stem Cell Res Ther, 2018;9(1):15. 2018 [PMID: 29361979] (Bioassay, Human) Bioassay Human
NV Dorrello, BA Guenthart, JD O'Neill, J Kim, K Cunningham, YW Chen, M Biscotti, T Swayne, HM Wobma, SXL Huang, HW Snoeck, M Bacchetta, G Vunjak-Nov Functional vascularized lung grafts for lung bioengineering Sci Adv, 2017;3(8):e1700521. 2017 [PMID: 28875163] (Bioassay, Human) Bioassay Human
KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors Nat Commun, 2017;8(1):331. 2017 [PMID: 28835709] (Bioassay, Human) Bioassay Human
AJ Jimenez-Ca, R Pillich, W Yang, GR Diaferia, P Meda, L Crisa, V Cirulli ?E-Catenin Is a Positive Regulator of Pancreatic Islet Cell Lineage Differentiation Cell Rep, 2017;20(6):1295-1306. 2017 [PMID: 28793255] (Bioassay, Mouse) Bioassay Mouse
CR Ball, F Oppel, KR Ehrenberg, TD Dubash, SM Dieter, CM Hoffmann, U Abel, F Herbst, M Koch, J Werner, F Bergmann, N Ishaque, M Schmidt, C von Kalle, C Scholl, S Fröhling, B Brors, W Weichert, J Weitz, H Glimm Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts EMBO Mol Med, 2017;0(0):. 2017 [PMID: 28526679] (Bioassay, Human) Bioassay Human
E El Agha, V Kheirollah, A Moiseenko, W Seeger, S Bellusci Ex vivo analysis of the contribution of FGF10(+) cells to airway smooth muscle cell formation during early lung development Dev. Dyn., 2017;0(0):. 2017 [PMID: 28387977] (Bioassay, Mouse) Bioassay Mouse
KB McCauley, F Hawkins, M Serra, DC Thomas, A Jacob, DN Kotton Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28366587] (Bioassay, Human) Bioassay Human
Show All 44 Publications.

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Bioinformatics

Gene Symbol FGF10
Uniprot