Recombinant Human FGF-10 Protein, CF


Recombinant Human FGF-10 (Catalog # 345-FG/CF) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50 for this effect is 20‑100 ng/mL.
1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.
Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and cultured for 20-60 days in lung organoid expansion medium, which includes more

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Applications Bioactivity

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Recombinant Human FGF-10 Protein, CF Summary

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Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 20-100 ng/mL.
E. coli-derived human FGF-10 protein
Cys37-Ser208 & Gly41-Ser208
Accession #
N-terminal Sequence
Cys37 & Gly41
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
19-22 kDa, reducing conditions
Read Publications using
345-FG/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-10 Protein, CF

  • FGF10
  • FGF-10
  • fibroblast growth factor 10
  • Keratinocyte growth factor 2
  • KGF2
  • KGF-2
  • produced by fibroblasts of urinary bladder lamina propria


The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (8, 9), formation of the otic vesicle and chochlea (10), adipogenesis (11), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (12 - 15). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 10, 13 ‑ 15). The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung (8, 9). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF‑10 biology (7, 10, 14, 15). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (16).

  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Igarashi, M. et al. (1998) J. Biol. Chem. 273:13230.
  3. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
  4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Min, H. et al. (1998) Genes Dev. 12:3156.
  7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
  8. Bellusci, S. et al. (1997) Development 124:4867.
  9. Weaver, M. et al. (2000) Development 127:2695.
  10. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
  11. Sakaue, H. et al. (2002) Genes Dev. 16:908.
  12. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
  13. Mailleux, A.A. et al. (2002) Development 129:53.
  14. Makarenkova, H.P. et al. (2000) Development 127:2563.
  15. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
  16. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

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Publications for FGF-10 (345-FG/CF)(84)

We have publications tested in 5 confirmed species: Human, Mouse, Canine, Xenopus, Zebrafish.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, In Vivo.

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Showing Publications 1 - 10 of 84. Show All 84 Publications.
Publications using 345-FG/CF Applications Species
H Wang, B Keepers, Y Qian, Y Xie, M Colon, J Liu, L Qian Cross-lineage potential of Ascl1 uncovered by comparing diverse reprogramming regulatomes Cell Stem Cell, 2022;29(10):1491-1504.e9. 2022-01-01 [PMID: 36206732] (Bioassay, Mouse) Bioassay Mouse
Y Han, L Tan, T Zhou, L Yang, L Carrau, LA Lacko, M Saeed, J Zhu, Z Zhao, BE Nilsson-Pa, FT Lira Neto, C Cahir, AM Giani, JC Chai, Y Li, X Dong, D Moroziewic, NYSCF Glob, D Paull, T Zhang, S Koo, C Tan, R Danziger, Q Ba, L Feng, Z Chen, A Zhong, GJ Wise, JZ Xiang, H Wang, RE Schwartz, BR tenOever, SA Noggle, CM Rice, Q Qi, T Evans, S Chen A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants Cell Stem Cell, 2022;29(10):1475-1490.e6. 2022-01-01 [PMID: 36206731] (Bioassay, Human) Bioassay Human
T Jiang, W Hu, S Zhang, C Ren, S Lin, Z Zhou, H Wu, J Yin, L Tan Fibroblast growth factor 10 attenuates chronic obstructive pulmonary disease by protecting against glycocalyx impairment and endothelial apoptosis Respiratory Research, 2022;23(1):269. 2022-01-01 [PMID: 36183124] (Cell Culture, Human) Cell Culture Human
MC Jaboreck, JL Lühmann, M Mielenz, F Stanke, G Göhring, U Martin, R Olmer, S Merkert Generation of two TMEM16A knockout iPSC clones each from a healthy human iPSC line, from a Cystic Fibrosis patient specific line with p.Phe508del mutation and from the gene corrected iPSC line Stem Cell Research, 2022;64(0):102918. 2022-01-01 [PMID: 36162332] (Bioassay, Human) Bioassay Human
V Lungova, K Wendt, SL Thibeault Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling Oncogene, 2022;0(0):. 2022-01-01 [PMID: 35770504] (Bioassay, Human) Bioassay Human
A Chansaenro, C Adine, S Charoenlap, S Roytrakul, L Sariya, T Osathanon, S Rungarunle, G Urkasemsin, R Chaisupara, S Yodmuang, GR Souza, JN Ferreira Magnetic bioassembly platforms towards the generation of extracellular vesicles from human salivary gland functional organoids for epithelial repair Bioactive materials, 2022;18(0):151-163. 2022-01-01 [PMID: 35387159] (Bioassay, Human) Bioassay Human
S Imamura, K Yoshimoto, S Terada, K Takamuro, KI Kamei In vitro culture at 39��C during hepatic maturation of human ES cells facilitates hepatocyte-like cell functions Scientific Reports, 2022;12(1):5155. 2022-01-01 [PMID: 35338220] (Bioassay, Human) Bioassay Human
AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells Cell Stem Cell, 2021;0(0):. 2021-01-01 [PMID: 34856121] (Bioassay, Human) Bioassay Human
L Johnson Ch, H Lahlou, C Steinacher, S Assou, Y Messat, J Dudás, A Edge, B Crespo, M Crosier, C Sergi, A Schrott-Fi, A Zine Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs International Journal of Molecular Sciences, 2021;22(19):. 2021-01-01 [PMID: 34639189] (Bioassay, Human) Bioassay Human
K Gkatzis, P Panza, S Peruzzo, DY Stainier Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures Elife, 2021;10(0):. 2021-01-01 [PMID: 34586063] (Bioassay, Mouse) Bioassay Mouse
Show All 84 Publications.

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Gene Symbol FGF10