Recombinant Human Dectin-1/CLEC7A Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Dectin-1/CLEC7A Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated Laminarin (1, 3-beta -glucan) in a functional ELISA with an estimated K_D <5 nM.
• Source: Mouse myeloma cell line, NS0-derived human Dectin-1/CLEC7A protein
  Thr66-Met201, with an N-terminal 10-His tag
• Accession #: NP_072092
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: CLEC7A
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 16.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Dectin-1/CLEC7A Protein, CF

• Beta-glucan receptor
• BGR
• CD369
• CLEC7A
• CLECSF12
• CLECSF12DC-associated C-type lectin 1
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12
• C-type lectin domain family 7 member A
• C-type lectin domain family 7, member A
• C-type lectin superfamily member 12
• Dectin1
• Dectin-1
• DECTIN1CANDF4
• Dendritic cell-associated C-type lectin 1
• dendritic cell-associated C-type lectin-1
• hDectin-1
• lectin-like receptor 1

Background

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3 - 5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4⁺ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5 - 7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 - 13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15 - 16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29% - 39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
4. Yokota, K. et al. (2001) Gene 272:51.
5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Publications for Dectin-1/CLEC7A (1859-DC)(9)

Publications using 1859-DC

• E Michaud, L Waeckel, R Gayet, R Goguyer-De, B Chanut, F Jospin, K Bathany, M Monnoye, C Genet, A Prier, C Tokarski, P Gérard, X Roblin, N Rochereau, S Paul Alteration of microbiota antibody-mediated immune selection contributes to dysbiosis in inflammatory bowel diseases Embo Molecular Medicine, 2022-07-04;0(0):e15386. 2022-07-04 [PMID: 35785473] (Bioassay, Human)
• D Yamanaka, K Suzuki, M Kimura, F Oyama, Y Adachi Functionally modified chitotriosidase catalytic domain for chitin detection based on split-luciferase complementation Carbohydrate polymers, 2022-01-12;282(0):119125. 2022-01-12 [PMID: 35123762] (Bioassay, N/A)
• X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence LYSMD3: A mammalian pattern recognition receptor for chitin Cell Reports, 2021-07-20;36(3):109392. 2021-07-20 [PMID: 34289364] (Bioassay, Human)
• C Leclaire, K Lecointe, PA Gunning, S Tribolo, DW Kavanaugh, A Wittmann, D Latousakis, DA MacKenzie, N Kawasaki, N Juge Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins FASEB J., 2018-01-29;0(0):fj201700619R. 2018-01-29 [PMID: 29401627] (Bioassay, Human)
• JS Griffiths, A Thompson, M Stott, A Benny, NA Lewis, PR Taylor, J Forton, S Herrick, SJ Orr, EP McGreal Differential susceptibility of Dectin-1 isoforms to functional inactivation by neutrophil and fungal proteases FASEB J., 2018-01-22;0(0):fj201701145R. 2018-01-22 [PMID: 29401615] (Bioassay, Human)
• AJ Smith, B Graves, R Child, PJ Rice, Z Ma, DW Lowman, HE Ensley, KT Ryter, JT Evans, DL Williams Immunoregulatory Activity of the Natural Product Laminarin Varies Widely as a Result of Its Physical Properties J. Immunol., 2017-12-15;0(0):. 2017-12-15 [PMID: 29246954] (Bioassay)
• KP Bene, DW Kavanaugh, C Leclaire, AP Gunning, DA MacKenzie, A Wittmann, ID Young, N Kawasaki, E Rajnavolgy, N Juge Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors Front Microbiol, 2017-03-07;8(0):321. 2017-03-07 [PMID: 28326063] (Bioassay, Human)
• SC Kerr, GJ Fischer, M Sinha, O McCabe, JM Palmer, T Choera, F Yun Lim, M Wimmerova, SD Carrington, S Yuan, CA Lowell, S Oscarson, NP Keller, JV Fahy FleA Expression in Aspergillus fumigatus Is Recognized by Fucosylated Structures on Mucins and Macrophages to Prevent Lung Infection PLoS Pathog, 2016-04-08;12(4):e1005555. 2016-04-08 [PMID: 27058347] (Flow Cytometry, Fungus)
• Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans. PLoS ONE, 2015-03-18;10(3):e0119244. 2015-03-18 [PMID: 25786219] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CLEC7A
• Uniprot:
  • NP_072092()