Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

Recombinant Human DC‑SIGN/CD209 Fc Chimera (Catalog # 11265-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 0.700-7.00 µg/mL.

2 μg/lane of Recombinant Human DC‑SIGN/CD209 Fc Chimera Protein (Catalog # 11265-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-74 kDa and 120-150 kDa, respectively.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF Summary

• Additional Information: CHO-Expressed
• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED₅₀ for this effect is 0.700-7.00 µg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human DC-SIGN/CD209 protein
  

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Human DC-SIGN/CD209 (Lys62-Ala404) Accession # Q9NNX6.1
  N-terminus			C-terminus

• Accession #: Q9NNX6.1
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 65.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-74 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

• CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
• CD209 molecule
• CD209
• CDSIGNHIV gpl20-binding protein
• CLEC4L
• CLEC4LC-type lectin domain family 4 member L
• DCSIGN
• DC-SIGN
• DC-SIGN1
• DC-SIGN1C-type lectin domain family 4, member L
• DC-SIGNMGC129965
• Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca²⁺-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Publications for DC-SIGN/CD209 (11265-DC)(1)

Publication using 11265-DC

• Eckmair, B;Gao, C;Mehta, AY;Dutkiewicz, Z;Vanbeselaere, J;Cummings, RD;Paschinger, K;Wilson, IBH; Recognition of highly branched N-glycans of the porcine whipworm by the immune system bioRxiv : the preprint server for biology 2023-09-21 [PMID: 37790353] (Bioassay, N/A)

Bioinformatics

• Uniprot:
  • Q9NNX6.1()