DC-SIGN/CD209 Antibodies: Novus Biologicals

MAB161

DC-SIGN/CD209 Antibody (120507) [Unconjugated]

(1 Review)

100 μg

MAB161

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

DC-SIGN was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 0.5 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." alt="DC-SIGN was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 0.5 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

DC-SIGN was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 0.5 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

DC-SIGN was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 0.5 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cyoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

Human DC-SIGN and DC-SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # <a class=

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." class="big_lightbox">

Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # <a class=

FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." class="big_thumb" />

<b>Biological Strategies Validation. </b>Infectivity of KSHV is enhanced in the presence of DC-SIGN and DC-SIGNR.A) 293T cells were transfected with empty pcDNA3 vector or expression constructs for DC-SIGN or DC-SIGNR. After 24 hours, cells were infected with 20 µl Bac16 delta K3 delta K5 or left uninfected as controls. Cells were harvested after additional 24 hours and surface stained with a DC-SIGN/R antibody (H-200) and analyzed by flow cytometry. Top three panels show transfected cells stained for DC-SIGN/R followed by PE- (DC-SIGN), FITC- (DC-SIGNR) or both (vector) conjugated secondary antibodies. Bottom panels shows KSHV infection of 293T cells transiently expressing DC-SIGN or DC-SIGNR. B, left panels) 293 cell lines stably expressing a vector construct, DC-SIGN or DC-SIGNR were fluorescently stained for surface expression of DC-SIGN or DC-SIGNR. The mean channel fluorescence is indicated in the upper right hand corner. Open histograms – secondary antibody alone; shaded histograms – DC-SIGN or DC-SIGNR staining. B, right panel) 293 pcDNA3, DC-SIGN or DC-SIGNR stable cell lines were pre-incubated with a control antibody (anti-ICAM1, 7 µg/ml), with mannan (100 µg/ml) or a monoclonal antibody specific for DC-SIGN (MAB161; 7 µg/ml) for 30 minutes on ice. These cells were then infected with wild type KSHV (Bac16 or rKSHV.219) at an MOI of 0.01. After 72 hours cells were harvested and evaluated for infection by flow cytometry measuring GFP expression. Infection rates were normalized to 293 pcDNA3 cells treated with the control antibody. The fold increase in relative infectivity is indicated. Data are representative of four independent experiments with two performed in triplicate. C) 293 pcDNA3, DC-SIGN or DC-SIGNR stable lines were infected with 50 µl of concentrated Bac16 wildtype (wt), or mutants with deletion of K3 only ( delta K3), K5 only ( delta K5), or deletion of both K3 and K5 ( delta K3 delta K5) as indicated. At 72 hours post-infection, the cells were stained for surface expression of DC-SIGN, DC-SIGNR or MHC class I. GFP fluore

Rapid induction and persistence of DC-SIGN, S100 and CD68 post-vaccination in lymphoid tissue.Galleries of representative fields of A DC-SIGN and B CD68 staining in MLN at 3, 7, 10, 21, 125 d.p.i. C Representative fields of DC-SIGN signal tracking through the spleen 3–125 d.p.i. maximal from d3 in red pulp (rp) and marginal zones (mg) D Representative fields of splenic S100 levels illustrate similar staining intensity 3–125 d.p.i. predominantly in the germinal centres and red pulp. Staining intensities scoring were; pale yellow (no staining, −); yellow (very low, +); dark yellow (low, ++), red (medium, +++) and magenta (high, ++++). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25162725), licensed under a CC-BY license. Not internally tested by R&D Systems.

Infection of DC-SIGN+ cells in the lung.(A) Infection of the DC-SIGNhi, DC-SIGNlo and DC-SIGN- cells in BAL from MV-infected macaques, determined by detection of EGFP in flow cytometry at day 2–5 d.p.i. Each dot represents an individual animal. Lines indicate geometric means. (B) Macroscopic images from EGFP+ lung slices collected 3 d.p.i., cultured for additional 3,5,7 or 10 days. (C) Phenotype of cells migrating from the ex vivo cultured lung slice, collected from supernatant after 5 days of culturing (D) Phenotype of EGFP+ cells collected from lung slice medium. (E-F) DC-SIGN expression on lung sections from uninfected macaques (E) or 2.d.p.i. (F) Asterisks indicate DC-SIGN reactivity. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23227146), licensed under a CC-BY license. Not internally tested by R&D Systems.

K3 and K5 affect the stability of DC-SIGN and DC-SIGNR in a RING-CH domain-dependent mechanism.A) 293 cells stably expressing wild-type K3 or K5, or the RING-CH mutant of either viral protein were transiently transfected with 2 µg DC-SIGN or DC-SIGNR constructs. At ∼48 hpt,cells were lysed in RIPA buffer and 30 µg of normalized lysate were loaded per sample. Protein levels of DC-SIGN or DC-SIGNR were determined by WB, and then blots were reprobed for lamin B as a loading control. Data is representative of at least three independent experiments. B) THP-1 cell stably expressing the indicated K5 constructs or empty vector were stained for cell surface levels of DC-SIGN. Solid histogram, empty vector; grey histogram, K5 construct; dotted histogram, isotype control. C) The same THP-1 cell lines used in Panel B, were lysed and subjected to western blotting with either a DC-SIGN (H-200) or GAPDH (O411) antibody, as loading control. Data is representative of at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23460925), licensed under a CC-BY license. Not internally tested by R&D Systems.

DC-SIGN is expressed by DCs and a subset of macrophages in lymph nodes.(A) TBLN cells were stained for DC-SIGN in combination with DC and macrophage markers and analyzed by flow cytometry. Gray areas show negative controls (DC-SIGN single staining). Percentages of positive cells expressing the markers are annotated in the upper right corner. (B) DC-SIGN expression of CD11c+ and CD83+ cells in TBLNs. (C-D) Dual immunofluorescence staining of DC-SIGN (green) and MAC387 (red) in lung sections 2 or 3 d.p.i. (C i-iv) and axillary lymphoid tissue 4 d.p.i. (D). Nuclei are stained blue with Hoechst. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23227146), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB161U-100UG
Alexa Fluor 405	FAB161V-100UG
Alexa Fluor 488	FAB161G-100UG
Alexa Fluor 532	FAB161X
Alexa Fluor 594	FAB161T-100UG
Alexa Fluor 647	FAB161R-100UG
Alexa Fluor 700	FAB161N-100UG
Alexa Fluor 750	FAB161S-100UG
Alexa Fluor Plus 405	FAB161AFP405-100UG
Alexa Fluor Plus 488	FAB161AFP488-100UG
Alexa Fluor Plus 555	FAB161AFP555-100UG
Alexa Fluor Plus 594	FAB161AFP594-100UG
Alexa Fluor Plus 647	FAB161AFP647-100UG
Alexa Fluor Plus 680	FAB161AFP680-100UG
Allophycocyanin	FAB161A-025
Allophycocyanin	FAB161A-100
Allophycocyanin/Cy7	FAB161APCCY7
Biotin	FAB161B
CoraFluor 1	FAB161CL1
DyLight 350	FAB161D
DyLight 405	FAB161E
DyLight 488	FAB161K
DyLight 550	FAB161L
DyLight 594	FAB161M
DyLight 650	FAB161W
DyLight 680	FAB161Y
DyLight 755	FAB161Z
Fluorescein	FAB161F-025
Fluorescein	FAB161F-100
HRP	FAB161H
Janelia Fluor 525	FAB161JF525
Janelia Fluor 549	FAB161I
Janelia Fluor 646	FAB161J
Janelia Fluor 669	FAB161JF669
PE/Atto594	FAB161PEATT594
PE/Cy5.5	FAB161PECY55
PE/Cy7	FAB161PECY7
Phycoerythrin	FAB161P-100
Phycoerythrin	FAB161P-025
mFluor Violet 450 SE	FAB161MFV450
mFluor Violet 500 SE	FAB161MFV500
mFluor Violet 610 SE	FAB161MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB161N-100UG
Carrier Free	MAB161-SP
Carrier Free	MAB161-500
Carrier Free	FAB161P-100
Carrier Free	FAB161P-025
Carrier Free	FAB161F-100
Carrier Free	FAB161F-025
Carrier Free	FAB161A-100
Carrier Free	FAB161A-025
Carrier Free	FAB161V-100UG
Carrier Free	FAB161U-100UG
Carrier Free	FAB161T-100UG
Carrier Free	FAB161S-100UG
Carrier Free	FAB161R-100UG
Carrier Free	FAB161G-100UG

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DDX0207

DC-SIGN/CD209 Antibody (109H12.03) - Azide and BSA Free

(8 Publications)

0.1 mg

DDX0207

Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis of Alexa Fluor (R) 647 conjugate of DDX0207. FACS staining of CD14+ CD209+ cells. (CD14+ cells cultured 5 days with GM CSF and IL-10)

Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0207. A) Staining of DC-SIGN transfected HeLa cells. B) IF staining of DC-SIGN transfected cells with 109H12.03.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0207A488
Alexa Fluor 546	DDX0207A546
Alexa Fluor 647	DDX0207A647
Biotin	DDX0207B

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DDX0202P-100

DC-SIGN/CD209 Antibody (102E11.06) - Azide and BSA Free

(5 Publications)

0.1 mg

DDX0202P-100

Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (102E11.06) [DDX0202P-100] - Human tonsil frozen section

Flow Cytometry: DC-SIGN/CD209 Antibody (102E11.06) [DDX0202P-100]

Reactivity:

Hu, Mu, Ca

Species Glossary

Reactivity: Hu, Mu, Ca

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0202A488-100
Alexa Fluor 546	DDX0202A546-100
Alexa Fluor 647	DDX0202A647-100
Biotin	DDX0202B-100

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NBP2-27408

DC-SIGN/CD209 Antibody

0.1 mg

NBP2-27408

Reactivity:

Hu, Mu, Pm, Pm, Pm-Or, RM

Species Glossary

Reactivity: Hu, Mu, Pm, Pm, Pm-Or, RM

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6806A

Alt Catalog# IMG-6806A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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DDX0209

DC-SIGN/CD209 Antibody (111H2.02) - Azide and BSA Free

(5 Publications)

0.1 mg

DDX0209

Flow Cytometry: DC-SIGN/CD209 Antibody (111H2.02) [DDX0209] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0209. A) Staining of NIH3T3 transfected cells with 111H2.02. B) Endogenous expression revealed by Flow staining with 111H2.02.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0209A488
Alexa Fluor 647	DDX0209A647
Biotin	DDX0209B

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NBP1-77284

DC-SIGN/CD209 Antibody - BSA Free

0.1 mg

NBP1-77284

Immunohistochemistry: DC-SIGN/CD209 Antibody - BSA Free [NBP1-77284] - Immunohistochemistry of DC-SIGN/CD209 in human lymph node tissue with DC-SIGN/CD209 antibody at 10 ug/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5563

Alt Catalog# IMG-5563

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77284AF350
Alexa Fluor 405	NBP1-77284AF405
Alexa Fluor 488	NBP1-77284AF488
Alexa Fluor 532	NBP1-77284AF532
Alexa Fluor 594	NBP1-77284AF594
Alexa Fluor 647	NBP1-77284AF647
Alexa Fluor 700	NBP1-77284AF700
Alexa Fluor 750	NBP1-77284AF750
Alexa Fluor Plus 405	NBP1-77284AFP405
Alexa Fluor Plus 488	NBP1-77284AFP488
Alexa Fluor Plus 555	NBP1-77284AFP555
Alexa Fluor Plus 594	NBP1-77284AFP594
Alexa Fluor Plus 647	NBP1-77284AFP647
Alexa Fluor Plus 680	NBP1-77284AFP680
Allophycocyanin	NBP1-77284APC
Biotin	NBP1-77284B
CoraFluor 1	NBP1-77284CL1
DyLight 350	NBP1-77284UV
DyLight 405	NBP1-77284V
DyLight 488	NBP1-77284G
DyLight 550	NBP1-77284R
DyLight 594	NBP1-77284DL594
DyLight 650	NBP1-77284C
DyLight 680	NBP1-77284FR
DyLight 755	NBP1-77284IR
FITC	NBP1-77284F
HRP	NBP1-77284H
Janelia Fluor 525	NBP1-77284JF525
Janelia Fluor 549	NBP1-77284JF549
Janelia Fluor 646	NBP1-77284JF646
Janelia Fluor 669	NBP1-77284JF669
PE	NBP1-77284PE
PerCP	NBP1-77284PCP
mFluor Violet 450 SE	NBP1-77284MFV450
mFluor Violet 500 SE	NBP1-77284MFV500
mFluor Violet 610 SE	NBP1-77284MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77284-0.025mg

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DDX0205P-100

DC-SIGN/CD209 Antibody (102F10.04) - Azide and BSA Free

(3 Publications)

0.1 mg

DDX0205P-100

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0205A488-100
Alexa Fluor 546	DDX0205A546-100
Alexa Fluor 647	DDX0205A647-100

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NBP2-53275

DC-SIGN/CD209 Antibody (C209/1781)

100 μg

NBP2-53275

Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) [NBP2-53275] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD209 Monoclonal Antibody (C209/1781).

Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (C209/1781) [NBP2-53275] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD209 Monoclonal Antibody (C209/1781).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54351AF350
Alexa Fluor 405	NBP2-54351AF405
Alexa Fluor 488	NBP2-54351AF488
Alexa Fluor 532	NBP2-54351AF532
Alexa Fluor 594	NBP2-54351AF594
Alexa Fluor 647	NBP2-54351AF647
Alexa Fluor 700	NBP2-54351AF700
Alexa Fluor 750	NBP2-54351AF750
Alexa Fluor Plus 405	NBP2-54351AFP405
Alexa Fluor Plus 488	NBP2-54351AFP488
Alexa Fluor Plus 555	NBP2-54351AFP555
Alexa Fluor Plus 594	NBP2-54351AFP594
Alexa Fluor Plus 647	NBP2-54351AFP647
Alexa Fluor Plus 680	NBP2-54351AFP680
Allophycocyanin	NBP2-54351APC
Biotin	NBP2-54351B
CoraFluor 1	NBP2-54351CL1
DyLight 350	NBP2-54351UV
DyLight 405	NBP2-54351V
DyLight 488	NBP2-54351G
DyLight 550	NBP2-54351R
DyLight 594	NBP2-54351DL594
DyLight 650	NBP2-54351C
DyLight 680	NBP2-54351FR
DyLight 755	NBP2-54351IR
FITC	NBP2-54351F
HRP	NBP2-54351H
Janelia Fluor 525	NBP2-54351JF525
Janelia Fluor 549	NBP2-54351JF549
Janelia Fluor 646	NBP2-54351JF646
Janelia Fluor 669	NBP2-54351JF669
PE	NBP2-54351PE
mFluor Violet 450 SE	NBP2-54351MFV450
mFluor Violet 500 SE	NBP2-54351MFV500
mFluor Violet 610 SE	NBP2-54351MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54351-100ug

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AF161

DC-SIGN/CD209 Antibody [Unconjugated]

100 μg

AF161

HAF019). A specific band was detected for DC-SIGN/CD209 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of human dendritic cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human DC-SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for DC-SIGN/CD209 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human dendritic cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human DC-SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DC-SIGN/CD209 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human DC‑SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human immature dendritic cells." class="big_lightbox">

<b>Left:</b> Simple Western lane view shows lysates of human immature dendritic cells, loaded at 0.4 mg/ml. A specific band was detected for DC‑SIGN/CD209 at approximately 59 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Sheep Anti-Human DC‑SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human DC‑SIGN/CD209 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF161) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human immature dendritic cells." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF161U-100UG
Alexa Fluor 405	AF161V-100UG
Alexa Fluor 488	AF161G-100UG
Alexa Fluor 532	AF161X-100UG
Alexa Fluor 594	AF161T-100UG
Alexa Fluor 647	AF161R-100UG
Alexa Fluor 700	AF161N-100UG
Alexa Fluor 750	AF161S-100UG
Alexa Fluor Plus 405	AF161AFP405-100UG
Alexa Fluor Plus 488	AF161AFP488-100UG
Alexa Fluor Plus 555	AF161AFP555-100UG
Alexa Fluor Plus 594	AF161AFP594-100UG
Alexa Fluor Plus 647	AF161AFP647-100UG
Alexa Fluor Plus 680	AF161AFP680-100UG
Allophycocyanin	AF161A
Biotin	AF161B
CoraFluor 1	AF161CL1
DyLight 350	AF161D
DyLight 405	AF161E
DyLight 488	AF161K
DyLight 550	AF161L
DyLight 594	AF161M
DyLight 650	AF161W
DyLight 680	AF161Y
DyLight 755	AF161Z
FITC	AF161F
HRP	AF161H
Janelia Fluor 525	AF161JF525
Janelia Fluor 549	AF161I
Janelia Fluor 646	AF161J
Janelia Fluor 669	AF161JF669
PE	AF161P
PerCP	AF161C
mFluor Violet 450 SE	AF161MFV450
mFluor Violet 500 SE	AF161MFV500
mFluor Violet 610 SE	AF161MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF161-SP

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MAB83451

DC-SIGN/CD209 Antibody (MMD3) [Unconjugated]

(1 Publication)

100 μg

MAB83451

F0101B)." class="big_lightbox">

CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209 was stained with Mouse Anti-Mouse DC-SIGN/CD209 Monoclonal Antibody (Catalog # MAB83451, filled histogram) or isotype control antibody Mouse IgG<sub>2C</sub>, followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

F0101B)." alt="CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209 was stained with Mouse Anti-Mouse DC-SIGN/CD209 Monoclonal Antibody (Catalog # MAB83451, filled histogram) or isotype control antibody Mouse IgG_2C, followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB83451U-100UG
Alexa Fluor 405	FAB83451V-100UG
Alexa Fluor 488	FAB83451G-100UG
Alexa Fluor 532	FAB83451X
Alexa Fluor 594	FAB83451T-100UG
Alexa Fluor 647	FAB83451R-100UG
Alexa Fluor 700	FAB83451N-100UG
Alexa Fluor 750	FAB83451S-100UG
Alexa Fluor Plus 405	FAB83451AFP405-100UG
Alexa Fluor Plus 488	FAB83451AFP488-100UG
Alexa Fluor Plus 555	FAB83451AFP555-100UG
Alexa Fluor Plus 594	FAB83451AFP594-100UG
Alexa Fluor Plus 647	FAB83451AFP647-100UG
Alexa Fluor Plus 680	FAB83451AFP680-100UG
Allophycocyanin	FAB83451A-025
Allophycocyanin/Cy7	FAB83451APCCY7
Biotin	FAB83451B
CoraFluor 1	FAB83451CL1
DyLight 350	FAB83451D
DyLight 405	FAB83451E
DyLight 488	FAB83451K
DyLight 550	FAB83451L
DyLight 594	FAB83451M
DyLight 650	FAB83451W
DyLight 680	FAB83451Y
DyLight 755	FAB83451Z
FITC	FAB83451F
HRP	FAB83451H
Janelia Fluor 525	FAB83451JF525
Janelia Fluor 549	FAB83451I
Janelia Fluor 646	FAB83451J
Janelia Fluor 669	FAB83451JF669
PE	FAB83451P
PE/Atto594	FAB83451PEATT594
PE/Cy5.5	FAB83451PECY55
PE/Cy7	FAB83451PECY7
PerCP	FAB83451C
mFluor Violet 450 SE	FAB83451MFV450
mFluor Violet 500 SE	FAB83451MFV500
mFluor Violet 610 SE	FAB83451MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB83451-SP
Carrier Free	FAB83451A-025
Carrier Free	FAB83451V-100UG
Carrier Free	FAB83451U-100UG
Carrier Free	FAB83451T-100UG
Carrier Free	FAB83451S-100UG
Carrier Free	FAB83451R-100UG
Carrier Free	FAB83451N-100UG
Carrier Free	FAB83451G-100UG

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NBP1-43328

DC-SIGN/CD209 Antibody (LWC06) - BSA Free

(1 Publication)

0.1 mg

NBP1-43328

Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the Allophycocyanin conjugate of NBP1-43328. Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.125 ug of Rat IgG2a kappa Isotype Control APC (blue histogram) or 0.125 ug of Anti-Mouse CD209 (DC-SIGN) APC (purple histogram).

Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the FITC conjugate of NBP1-43328. Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.5 ug of Rat IgG2a kappa Isotype Control FITC (open histogram) or 0.5 ug of Anti-Mouse CD209 (DC-SIGN) FITC (filled histogram).

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-43328AF350
Alexa Fluor 405	NBP1-43328AF405
Alexa Fluor 488	NBP1-43328AF488
Alexa Fluor 532	NBP1-43328AF532
Alexa Fluor 594	NBP1-43328AF594
Alexa Fluor 647	NBP1-43328AF647
Alexa Fluor 700	NBP1-43328AF700
Alexa Fluor 750	NBP1-43328AF750
Alexa Fluor Plus 405	NBP1-43328AFP405
Alexa Fluor Plus 488	NBP1-43328AFP488
Alexa Fluor Plus 555	NBP1-43328AFP555
Alexa Fluor Plus 594	NBP1-43328AFP594
Alexa Fluor Plus 647	NBP1-43328AFP647
Alexa Fluor Plus 680	NBP1-43328AFP680
Allophycocyanin	NBP1-43328APC
Allophycocyanin/Cy7	NBP1-43328APCCY7
Biotin	NBP1-43328B
CoraFluor 1	NBP1-43328CL1
DyLight 350	NBP1-43328UV
DyLight 405	NBP1-43328V
DyLight 488	NBP1-43328G
DyLight 550	NBP1-43328R
DyLight 594	NBP1-43328DL594
DyLight 650	NBP1-43328C
DyLight 680	NBP1-43328FR
DyLight 755	NBP1-43328IR
FITC	NBP1-43328F
HRP	NBP1-43328H
Janelia Fluor 525	NBP1-43328JF525
Janelia Fluor 549	NBP1-43328JF549
Janelia Fluor 646	NBP1-43328JF646
Janelia Fluor 669	NBP1-43328JF669
PE	NBP1-43328PE
PE/Atto594	NBP1-43328PEATT594
PE/Cy5.5	NBP1-43328PECY55
PE/Cy7	NBP1-43328PECY7
PerCP	NBP1-43328PCP
mFluor Violet 450 SE	NBP1-43328MFV450
mFluor Violet 500 SE	NBP1-43328MFV500
mFluor Violet 610 SE	NBP1-43328MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-43328-0.025mg

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MAB8345

DC-SIGN/CD209 Antibody (902404) [Unconjugated]

100 μg

MAB8345

MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_lightbox">

CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209 was stained with Rat Anti-Mouse DC-SIGN/CD209 Monoclonal Antibody (Catalog # MAB8345, filled histogram) or isotype control antibody (Catalog # <a class=

MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." alt="CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209 was stained with Rat Anti-Mouse DC-SIGN/CD209 Monoclonal Antibody (Catalog # MAB8345, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB8345U-100UG
Alexa Fluor 405	FAB8345V-100UG
Alexa Fluor 488	FAB8345G-100UG
Alexa Fluor 532	FAB8345X
Alexa Fluor 594	FAB8345T-100UG
Alexa Fluor 647	FAB8345R-100UG
Alexa Fluor 700	FAB8345N-100UG
Alexa Fluor 750	FAB8345S-100UG
Alexa Fluor Plus 405	FAB8345AFP405-100UG
Alexa Fluor Plus 488	FAB8345AFP488-100UG
Alexa Fluor Plus 555	FAB8345AFP555-100UG
Alexa Fluor Plus 594	FAB8345AFP594-100UG
Alexa Fluor Plus 647	FAB8345AFP647-100UG
Alexa Fluor Plus 680	FAB8345AFP680-100UG
Allophycocyanin	FAB8345A
Allophycocyanin/Cy7	FAB8345APCCY7
Biotin	FAB8345B
CoraFluor 1	FAB8345CL1
DyLight 350	FAB8345D
DyLight 405	FAB8345E
DyLight 488	FAB8345K
DyLight 550	FAB8345L
DyLight 594	FAB8345M
DyLight 650	FAB8345W
DyLight 680	FAB8345Y
DyLight 755	FAB8345Z
FITC	FAB8345F
HRP	FAB8345H
Janelia Fluor 525	FAB8345JF525
Janelia Fluor 549	FAB8345I
Janelia Fluor 646	FAB8345J
Janelia Fluor 669	FAB8345JF669
PE/Atto594	FAB8345PEATT594
PE/Cy5.5	FAB8345PECY55
PE/Cy7	FAB8345PECY7
PerCP	FAB8345C
Phycoerythrin	FAB8345P-025
mFluor Violet 450 SE	FAB8345MFV450
mFluor Violet 500 SE	FAB8345MFV500
mFluor Violet 610 SE	FAB8345MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB8345-SP
Carrier Free	FAB8345P-025
Carrier Free	FAB8345V-100UG
Carrier Free	FAB8345U-100UG
Carrier Free	FAB8345T-100UG
Carrier Free	FAB8345S-100UG
Carrier Free	FAB8345R-100UG
Carrier Free	FAB8345N-100UG
Carrier Free	FAB8345G-100UG

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

DC-SIGN/CD209 Antibodies

DC-SIGN/CD209 Antibody (120507) [Unconjugated]

DC-SIGN/CD209 Antibody (109H12.03) - Azide and BSA Free

DC-SIGN/CD209 Antibody (102E11.06) - Azide and BSA Free

DC-SIGN/CD209 Antibody

DC-SIGN/CD209 Antibody (111H2.02) - Azide and BSA Free

DC-SIGN/CD209 Antibody - BSA Free

DC-SIGN/CD209 Antibody (102F10.04) - Azide and BSA Free

DC-SIGN/CD209 Antibody (C209/1781)

DC-SIGN/CD209 Antibody [Unconjugated]

DC-SIGN/CD209 Antibody (MMD3) [Unconjugated]

DC-SIGN/CD209 Antibody (LWC06) - BSA Free

DC-SIGN/CD209 Antibody (902404) [Unconjugated]