CD83 Antibody (HB15e) [Unconjugated]

Images

 
Human mature dendritic cells were stained with Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated ...read more
CD83 was detected in immersion fixed human dendritic cells using 10 µg/mL Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774) for 3 hours at room temperature. Cells were stained with the NorthernLights™ ...read more
CD83 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774) at 8 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Daudi cells were stained with Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
HB15e
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD83 Antibody (HB15e) [Unconjugated] Summary

Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cells transfected with human CD83
Specificity
Detects human CD83.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD83
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB1774 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD83 Antibody (HB15e) [Unconjugated]

  • B-cell activation protein
  • BL11
  • BL11CD83 antigen
  • CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)
  • CD83 molecule
  • CD83
  • Cell surface protein HB15
  • cell-surface glycoprotein
  • HB15
  • HB15hCD83

Background

Human CD83 is a 40‑50 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). Although in vitro studies suggest CD83 may form membrane-bound covalent homodimers, in vivo this does not appear to be the case (1, 4). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4, 5). Relative to human, mouse CD83 is 11 aa shorter in its extracellular domain and is expressed as a 30‑35 kDa protein (1, 4, 5). Human CD83 is active in the mouse system (4). One alternate splice form has been reported. This leads to a small monomeric soluble form of 74 aa that includes aa 20‑52 and aa 164‑205 (6, 7). In human, proteolytic cleavage and solubilization of CD83 has also been suggested, and this could lead to dimeric circulating CD83 (4, 6). CD83 is a primary marker for dendritic cells (3, 6, 8). It is also found on B cells (6, 9), neutrophils (10), monocytes and macrophages (11). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells (12). Membrane CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (13, 14). Soluble CD83, however, appears to be immunosuppressive and blocks T cell activation (15, 16). On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype (13). A lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (17).

  1. Zhou, L-J. et al. (1992) J. Immunol. 149:735.
  2. Kozlow, E.J. et al. (1993) Blood 81:454.
  3. Fujimoto, Y and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
  4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
  5. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
  6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
  7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
  8. Velten, F.W. et al. (2007) Mol. Immunol. 44:1544.
  9. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
  10. Yamashiro, S. et al. (2000) Blood 96:3958.
  11. Cao, W. et al. (2005) Biochem. J. 385:85.
  12. Scholler, N. et al. (2001) J. Immunol. 166:3865.
  13. Scholler, N. et al. (2002) J. Immunol. 168:2599.
  14. Hirano, N. et al. (2006) Blood 107:1528.
  15. Kotzor, N. et al. (2004) Immunobiology 209:129.
  16. Zinser, E. et al. (2006) Immunobiology 211:449.
  17. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD83 Antibody (MAB1774)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC-Fr, IHC-P, Neutralization.


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IHC-Fr
(1)
IHC-P
(2)
Neutralization
(1)
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Human
(4)
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Showing Publications 1 - 6 of 6.
Publications using MAB1774 Applications Species
Morten Hjuler Nielsen, Rikke Bæk, Malene Moller Jorgensen, Maiken Mellergaard, Aase Handberg Increased extracellular vesicles (EVs) related to T cell-mediated inflammation and vascular function in familial hypercholesterolemia Atherosclerosis Plus 2023-09-01 [PMID: 37637934]
SHI-XIA LIAO, TING DING, XI-MIN RAO, DE-SHENG SUN, PENG-PENG SUN, YA-JUN WANG et al. Cigarette smoke affects dendritic cell maturation in the small airways of patients with chronic obstructive pulmonary disease Molecular Medicine Reports 2015-01-01 [PMID: 25338516]
Izquierdo-Useros N, Lorizate M, Puertas M, Rodriguez-Plata M, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler O, Telenti A, Krausslich H, Martinez-Picado J Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides. PLoS Biol, 2012-12-18;10(12):e1001448. 2012-12-18 [PMID: 23271952] (Neutralization, Human) Neutralization Human
Tucci M, Quatraro C, Lombardi L, Pellegrino C, Dammacco F, Silvestris F Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18. Arthritis Rheum., 2008-01-01;58(1):251-62. 2008-01-01 [PMID: 18163476] (IHC-P, Human) IHC-P Human
Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua. J. Pathol., 2008-02-01;214(3):328-36. 2008-02-01 [PMID: 18069648] (IHC-Fr, Human) IHC-Fr Human
Tucci M, Quatraro C, Dammacco F, Silvestris F Interleukin-18 overexpression as a hallmark of the activity of autoimmune inflammatory myopathies. Clin. Exp. Immunol., 2006-10-01;146(1):21-31. 2006-10-01 [PMID: 16968394] (IHC-P, Human) IHC-P Human

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Bioinformatics

Gene Symbol CD83