DC-SIGN/CD209 Antibody (120507) [Unconjugated]


Human DC‑SIGN and DC‑SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody ...read more
Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready

Order Details

DC-SIGN/CD209 Antibody (120507) [Unconjugated] Summary

Additional Information
Clone 120507 was used by HLDA to establish CD designation
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
Dendritic Cell Marker
Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Adhesion Blockade 5.0 ug/mL
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
MAB161 in the following applications:

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MAB161 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-SIGN/CD209 Antibody (120507) [Unconjugated]

  • CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
  • CD209 molecule
  • CD209
  • CDSIGNHIV gpl20-binding protein
  • CLEC4L
  • CLEC4LC-type lectin domain family 4 member L
  • DC-SIGN1
  • DC-SIGN1C-type lectin domain family 4, member L
  • DC-SIGNMGC129965
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 1


Human DC-Sign (dendritic cell-specific ICAM-3 grabbing nonintegrin; also CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).

  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Su, S.V. et al. (2004) J. Biol. Chem. 279:19122.
  5. Cambi, A. et al. (2005) Cell. Microbiol. 7:481.
  6. Serrano-Gomez, D. et al. (2004) J. Immunol. 173:5635.
  7. Geijtenbeek, T.B.H. and Y. van Kooyk (2003) Curr. Top. Microbiol. Immunol. 276:32.
  8. Baribaud, F. et al. (2001) J. Virol. 75:10281.
  9. Wu, L. et al. (2002) J. Virol. 76:5905.
  10. Baribaud, F. et al. (2002) J. Virol.76:9135.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DC-SIGN/CD209 Antibody (MAB161)(40)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 10 applications: Blocking, ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.

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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using MAB161 Applications Species
N Palmerin, F Aalam, R Nabiee, M Muniraju, JG Ogembo, J Totonchy Suppression of DC-SIGN and gH Reveals Complex, Subset-Specific Mechanisms for KSHV Entry in Primary B Lymphocytes Viruses, 2021-07-31;13(8):. 2021-07-31 [PMID: 34452377] (Neutralization, Human) Neutralization Human
P Zhao, LD Xu, Y Zhang, H Cao, R Chen, B Wang, YW Huang Expression of the human or porcine C-type lectins DC-SIGN/L-SIGN confers susceptibility to porcine epidemic diarrhea virus entry and infection in otherwise refractory cell lines Microbial pathogenesis, 2021-05-19;157(0):104956. 2021-05-19 [PMID: 34022357] (IHC, Hamster) IHC Hamster
C Herrera, MD McRaven, KG Laing, J Dennis, TJ Hope, RJ Shattock Early Colorectal Responses to HIV-1 and Modulation by Antiretroviral Drugs Vaccines, 2021-03-08;9(3):. 2021-03-08 [PMID: 33800213] (IHC, Human) IHC Human
B Hu, Z Wang, H Zeng, Y Qi, Y Chen, T Wang, J Wang, Y Chang, Q Bai, Y Xia, Y Wang, L Liu, Y Zhu, B Dai, J Guo, L Xu, W Zhang, J Xu Blockade of DC-SIGN+ tumor-associated macrophages reactivates anti-tumor immunity and improves immunotherapy in muscle-invasive bladder cancer Cancer Res., 2020-02-14;0(0):. 2020-02-14 [PMID: 32060149] (Neutralization, Human) Neutralization Human
MB Vogt, A Lahon, RP Arya, JL Spencer Cl, R Rico-Hesse Dengue viruses infect human megakaryocytes, with probable clinical consequences PLoS Negl Trop Dis, 2019-11-25;13(11):e0007837. 2019-11-25 [PMID: 31765380] (Flow Cytometry, Human) Flow Cytometry Human
M Xie, H Leroy, R Mascarau, M Woottum, M Dupont, C Ciccone, A Schmitt, B Raynaud-Me, C Vérollet, J Bouchet, L Bracq, S Benichou Cell-to-Cell Spreading of HIV-1 in Myeloid Target Cells Escapes SAMHD1 Restriction MBio, 2019-11-19;10(6):. 2019-11-19 [PMID: 31744918] (Flow Cytometry, Human) Flow Cytometry Human
S Márquez, JJ Fernández, C Mancebo, C Herrero-Sá, S Alonso, TA Sandoval, M Rodríguez, JR Cubillos-R, O Montero, N Fernández, M Sánchez Cr Tricarboxylic Acid Cycle Activity and Remodeling of Glycerophosphocholine Lipids Support Cytokine Induction in Response to Fungal Patterns Cell Rep, 2019-04-09;27(2):525-536.e4. 2019-04-09 [PMID: 30970255] (ICC, Human) ICC Human
KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-? Eur. J. Immunol, 2017-03-01;0(0):. 2017-03-01 [PMID: 28105653] (Flow Cytometry, Human) Flow Cytometry Human
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro PLoS ONE, 2016-11-29;11(11):e0167410. 2016-11-29 [PMID: 27898740] (Neutralization, Human) Neutralization Human
Metformin Uniquely Prevents Thrombosis by Inhibiting Platelet Activation and mtDNA Release Sci Rep, 2016-11-02;6(0):36222. 2016-11-02 [PMID: 27805009] (Neutralization, Rat) Neutralization Rat
Show All 40 Publications.

Review for DC-SIGN/CD209 Antibody (MAB161) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB161:
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Images Ratings Applications Species Date Details
Flow Cytometry DC-SIGN/CD209 MAB161
reviewed by:
Verified Customer
Flow Human 01/05/2018


ApplicationFlow Cytometry
Sample TestedDCSIGN Transfected HEK293T


CommentsHEK293T cells were transfected with pcDNA3.DC-SIGN, 48hrs post-transfection cells were analyzed by flow cytometry. A 1/500 dilution of antibody was used to stain transfected cells (~2E6 cells).

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Recent Reviews


Verified Customer
Application: Flow
Species: Human


Gene Symbol CD209