DC-SIGN/CD209 Antibody (120507)

Images

 
Human DC‑SIGN and DC‑SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody ...read more
Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 ...read more
DC-SIGN was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, AdBlk, CyTOF-ready, ICC
Clone
120507
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

DC-SIGN/CD209 Antibody (120507) Summary

Additional Information
Clone 120507 was used by HLDA to establish CD designation
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
Marker
Dendritic Cell Marker
Specificity
Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD209
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Adhesion Blockade 5.0 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB161 in the following applications:

Publications
Read Publications using
MAB161 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-SIGN/CD209 Antibody (120507)

  • CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
  • CD209 molecule
  • CD209
  • CDSIGNHIV gpl20-binding protein
  • CLEC4L
  • CLEC4LC-type lectin domain family 4 member L
  • DCSIGN
  • DC-SIGN
  • DC-SIGN1
  • DC-SIGN1C-type lectin domain family 4, member L
  • DC-SIGNMGC129965
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

Human DC-Sign (dendritic cell-specific ICAM-3 grabbing nonintegrin; also CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DC-SIGN/CD209 Antibody (MAB161)(33)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 10 applications: Blocking, ELISA Development, Flow, Functional Assay, ICC, IHC, IHC Frozen, IHC Paraffin-embedded, Neut, WB.


Filter By Application
Blocking
(2)
ELISA Development
(1)
Flow
(11)
Functional Assay
(1)
ICC
(4)
IHC
(1)
IHC Frozen
(2)
IHC Paraffin-embedded
(2)
Neut
(6)
WB
(2)
All Applications
Filter By Species
Human
(28)
Mouse
(1)
Rat
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using MAB161 Applications Species
KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-? Eur. J. Immunol, 2017;0(0):. 2017 [PMID: 28105653] (Flow,, Human) Flow Human
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro PLoS ONE, 2016;11(11):e0167410. 2016 [PMID: 27898740] (Neut,, Human) Neut Human
Metformin Uniquely Prevents Thrombosis by Inhibiting Platelet Activation and mtDNA Release Sci Rep, 2016;6(0):36222. 2016 [PMID: 27805009] (Neut,, Rat) Neut Rat
Clearance of autophagy-associated dying retinal pigment epithelial cells - a possible source for inflammation in age-related macular degeneration Cell Death Dis, 2016;7(9):e2367. 2016 [PMID: 27607582] (Flow,, Human) Flow Human
HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses Sci Rep, 2016;6(0):29447. 2016 [PMID: 27385120] (Functional Assay,, Human) Functional Assay Human
Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production. PLoS ONE, 2015;10(3):e0120948. 2015 [PMID: 25793979] (Blocking,, Human) Blocking Human
El-Awady, Ahmed R, Miles, Brodie, Scisci, Elizabet, Kurago, Zoya B, Palani, Chithra, Arce, Roger M, Waller, Jennifer, Genco, Caroline, Slocum, Connie, Manning, Matthew, Schoenlein, Patricia, Cutler, Christop Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk. PLoS Pathog, 2015;10(2):e1004647. 2015 [PMID: 25679217] (Flow,, Human) Flow Human
Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection. PLoS Pathog, 2014;10(12):e1004548. 2014 [PMID: 25474532] (Blocking,, Human) Blocking Human
Rennick L, de Vries R, Carsillo T, Lemon K, van Amerongen G, Ludlow M, Nguyen D, Yuksel S, Verburgh R, Haddock P, McQuaid S, Duprex W, de Swart R Live-attenuated measles virus vaccine targets dendritic cells and macrophages in muscle of nonhuman primates. J Virol, 2015;89(4):2192-200. 2015 [PMID: 25473055]
Ferguson D, Mattiuzzo G, Ham C, Stebbings R, Li B, Rose N, Mee E, Smith D, Page M, Cranage M, Almond N, Towers G, Berry N Early biodistribution and persistence of a protective live attenuated SIV vaccine elicits localised innate responses in multiple lymphoid tissues. PLoS ONE, 2014;9(8):e104390. 2014 [PMID: 25162725] (IHC Paraffin-embedded,, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) IHC Paraffin-embedded Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Show All 33 Publications.

Review for DC-SIGN/CD209 Antibody (MAB161) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB161:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry DC-SIGN/CD209 MAB161
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reviewed by:
Anonymous
Flow Human 01/05/2018
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Summary

ApplicationFlow Cytometry
Sample TestedDCSIGN Transfected HEK293T
SpeciesHuman
Lot1460467

Comments

CommentsHEK293T cells were transfected with pcDNA3.DC-SIGN, 48hrs post-transfection cells were analyzed by flow cytometry. A 1/500 dilution of antibody was used to stain transfected cells (~2E6 cells).

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Recent Reviews

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5
1
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0
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1
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Anonymous
01/05/2018
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD209
Entrez