DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated]

Images

 
Human DC‑SIGNR/CD299 and DC‑SIGN transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGNR/CD299 Monoclonal Antibody (Catalog # MAB162, filled histograms) or isotype control ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
120604
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated] Summary

Additional Information
Clone 120604 was used by HLDA to establish CD designation
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Accession # Q9H2X3
Specificity
Detects human DC‑SIGNR/CD299.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CLEC4M
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Adhesion Blockade 2.5 ug/mL
  • CyTOF-ready
Publications
Read Publications using
MAB162 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated]

  • CD209L
  • CD209L1
  • CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2
  • CD299 antigenMGC129964
  • CD299
  • CLEC4M
  • C-type lectin domain family 4, member M
  • DC-SIGN2
  • DC-SIGN2CD209 antigen-like protein 1
  • DCSIGNR
  • DC-SIGNR
  • DC-SIGNRDC-SIGN-related protein
  • DCSIGNRMGC47866
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 2
  • HP10347
  • liver/lymph node-specific ICAM-3 grabbing non-integrin
  • Liver/lymph node-specific ICAM-3-grabbing non-integrin
  • LSIGN
  • L-SIGN
  • LSIGNC-type lectin domain family 4 member M
  • mannose binding C-type lectin DC-SIGNR

Background

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

  1. Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
  2. Pohlmann, S. et al. (2003) J. Virol. 77:4070.
  3. Simmons, L.G. et al. (2003) J. Virol. 77:1337.
  4. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
  5. Feinberg, H. et al. (2001) Science 294:2163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DC-SIGNR/CD299/CLEC4M Antibody (MAB162)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Neutralization.


Filter By Application
Flow Cytometry
(3)
ICC
(1)
IHC
(2)
IHC-Fr
(1)
Neutralization
(3)
All Applications
Filter By Species
Human
(10)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB162 Applications Species
MB Vogt, A Lahon, RP Arya, JL Spencer Cl, R Rico-Hesse Dengue viruses infect human megakaryocytes, with probable clinical consequences PLoS Negl Trop Dis, 2019;13(11):e0007837. 2019 [PMID: 31765380] (Flow Cytometry, Human) Flow Cytometry Human
LL Swystun, JD Lai, C Notley, I Georgescu, AS Paine, J Mewburn, K Nesbitt, K Schledzews, C Géraud, J Kzhyshkows, S Goerdt, W Hopman, RR Montgomery, PD James, D Lillicrap The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity J. Clin. Invest., 2018;128(9):4057-4073. 2018 [PMID: 30124466] (Flow Cytometry, Human) Flow Cytometry Human
Johnson TR, McLellan JS, Graham BS Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. J. Virol., 2012;86(3):1339-47. 2012 [PMID: 22090124] (Neutralization, Human) Neutralization Human
Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. J. Hepatol., 2009;51(4):734-49. 2009 [PMID: 19703720] (IHC, Human) IHC Human
Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells. J. Virol., 2009;83(21):10908-21. 2009 [PMID: 19692463] (Neutralization, Human) Neutralization Human
Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pohlmann S DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets. J. Virol., 2006;80(18):8951-60. 2006 [PMID: 16940507] (Neutralization, Human) Neutralization Human
Falkowska E, Durso R, Gardner J, Cormier E, Arrigale R, Ogawa R, Donovan G, Maddon P, Olson W, Dragic T L-SIGN (CD209L) isoforms differently mediate trans-infection of hepatoma cells by hepatitis C virus pseudoparticles. J Gen Virol, 0;87(0):2571-6. 0 [PMID: 16894195] (Flow Cytometry, Human) Flow Cytometry Human
Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction. Gut, 2006;56(2):243-52. 2006 [PMID: 16840505] (IHC-Fr, Human) IHC-Fr Human
Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles. Am. J. Pathol., 2006;169(1):200-8. 2006 [PMID: 16816373] (IHC, Human) IHC Human
Ludwig IS, Lekkerkerker AN, Depla E, Bosman F, Musters RJ, Depraetere S, van Kooyk Y, Geijtenbeek TB Hepatitis C virus targets DC-SIGN and L-SIGN to escape lysosomal degradation. J. Virol., 2004;78(15):8322-32. 2004 [PMID: 15254204] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol CLEC4M
Uniprot