DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated] Summary
Clone 120604 was used by HLDA to establish CD designation
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Accession # Q9H2X3
Detects human DC‑SIGNR/CD299.
Protein A or G purified from hybridoma culture supernatant
Test in a species/application not listed above to receive a full credit towards a future purchase.
Flow Cytometry 2.5 ug/10^6 cells Adhesion Blockade 2.5 ug/mL CyTOF-ready
Packaging, Storage & Formulations
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for DC-SIGNR/CD299/CLEC4M Antibody (120604) [Unconjugated]
CD209L CD209L1 CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2 CD299 antigenMGC129964 CD299 CLEC4M C-type lectin domain family 4, member M DC-SIGN2 DC-SIGN2CD209 antigen-like protein 1 DCSIGNR DC-SIGNR DC-SIGNRDC-SIGN-related protein DCSIGNRMGC47866 Dendritic cell-specific ICAM-3-grabbing non-integrin 2 HP10347 liver/lymph node-specific ICAM-3 grabbing non-integrin Liver/lymph node-specific ICAM-3-grabbing non-integrin LSIGN L-SIGN LSIGNC-type lectin domain family 4 member M mannose binding C-type lectin DC-SIGNR Background
Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).
et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670. Pohlmann, S.
et al. (2003) J. Virol. 77:4070. Simmons, L.G.
et al. (2003) J. Virol. 77:1337. Bahirova, A.A.
et al. (2001) J. Exp. Med. 193:671. Feinberg, H. et al. (2001) Science 294:2163. Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Applications: WB, Flow, IHC, AdBlk, CyTOF-ready, ICC
Species: Hu, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Po
Applications: Flow, ICC/IF, IF
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ha
Applications: WB, Simple Western, Flow, IHC, IP, Block
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Applications: WB, Block, ICC
Applications: IHC, IHC-P
Species: Hu, Mu, Bv, Fi
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Fe
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
Species: Hu, Mu, Gt, Pm, Sh
Applications: WB, DB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Applications: WB, ICC/IF, IHC, IHC-P
Publications for DC-SIGNR/CD299/CLEC4M Antibody (MAB162)(10)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Neutralization.
Submit a Publication
Filter By Application
Filter By Species
Showing Publications 1 -
Publications using MAB162
MB Vogt, A Lahon, RP Arya, JL Spencer Cl, R Rico-Hesse Dengue viruses infect human megakaryocytes, with probable clinical consequences PLoS Negl Trop Dis, 2019;13(11):e0007837. 2019 [PMID: 31765380] (Flow Cytometry, Human) Flow Cytometry
LL Swystun, JD Lai, C Notley, I Georgescu, AS Paine, J Mewburn, K Nesbitt, K Schledzews, C Géraud, J Kzhyshkows, S Goerdt, W Hopman, RR Montgomery, PD James, D Lillicrap The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity J. Clin. Invest., 2018;128(9):4057-4073. 2018 [PMID: 30124466] (Flow Cytometry, Human) Flow Cytometry
Johnson TR, McLellan JS, Graham BS Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. J. Virol., 2012;86(3):1339-47. 2012 [PMID: 22090124] (Neutralization, Human) Neutralization
Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. J. Hepatol., 2009;51(4):734-49. 2009 [PMID: 19703720] (IHC, Human) IHC
Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells. J. Virol., 2009;83(21):10908-21. 2009 [PMID: 19692463] (Neutralization, Human) Neutralization
Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pohlmann S DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets. J. Virol., 2006;80(18):8951-60. 2006 [PMID: 16940507] (Neutralization, Human) Neutralization
Falkowska E, Durso R, Gardner J, Cormier E, Arrigale R, Ogawa R, Donovan G, Maddon P, Olson W, Dragic T L-SIGN (CD209L) isoforms differently mediate trans-infection of hepatoma cells by hepatitis C virus pseudoparticles. J Gen Virol, 0;87(0):2571-6. 0 [PMID: 16894195] (Flow Cytometry, Human) Flow Cytometry
Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction. Gut, 2006;56(2):243-52. 2006 [PMID: 16840505] (IHC-Fr, Human) IHC-Fr
Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles. Am. J. Pathol., 2006;169(1):200-8. 2006 [PMID: 16816373] (IHC, Human) IHC
Ludwig IS, Lekkerkerker AN, Depla E, Bosman F, Musters RJ, Depraetere S, van Kooyk Y, Geijtenbeek TB Hepatitis C virus targets DC-SIGN and L-SIGN to escape lysosomal degradation. J. Virol., 2004;78(15):8322-32. 2004 [PMID: 15254204] (ICC, Human) ICC
DC-SIGNR/CD299/CLEC4M Antibody (MAB162) (0)
There are no reviews for DC-SIGNR/CD299/CLEC4M Antibody (MAB162).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for DC-SIGNR/CD299/CLEC4M Antibody (MAB162) (0)
Other Available Formats
Additional DC-SIGNR/CD299/CLEC4M Products
Bioinformatics Tool for DC-SIGNR/CD299/CLEC4M Antibody (MAB162)
Discover related pathways, diseases and genes to DC-SIGNR/CD299/CLEC4M Antibody (MAB162). Need help?
Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on DC-SIGNR/CD299/CLEC4M