Recombinant Human CNTF Protein, CF

Images

 
1 μg/lane of Recombinant Human CNTF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 26 kDa.
Recombinant Human CNTF (Catalog #257‑NT/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.03-0.18 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human CNTF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.03-0.18 μg/mL.
The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR) .
Source
E. coli-derived human CNTF protein
Ala2-Met200
Accession #
N-terminal Sequence
Ala2
Structure / Form

This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2-22.8 kDa.

Protein/Peptide Type
Recombinant Proteins
Gene
CNTF
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23-26 kDa, reducing conditions
Publications
Read Publications using
257-NT/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CNTF Protein, CF

  • ciliary neurotrophic factor
  • CNTF
  • HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

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Publications for CNTF (257-NT/CF)(44)

We have publications tested in 5 confirmed species: Human, Mouse, Canine, Chicken, Mousee.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(32)
Cell Culture
(7)
ELISA (Capture)
(1)
In Vivo
(2)
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Human
(33)
Mouse
(2)
Canine
(1)
Chicken
(7)
Mousee
(1)
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Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using 257-NT/CF Applications Species
H Ozaki, H Suga, M Sakakibara, M Soen, N Miyake, T Miwata, S Taga, T Nagai, M Kano, K Mitsumoto, T Miyata, T Kobayashi, M Sugiyama, T Onoue, H Takagi, D Hagiwara, S Iwama, R Banno, G Iguchi, Y Takahashi, K Muguruma, H Inoue, H Arima Differentiation of human induced pluripotent stem cells into hypothalamic vasopressin neurons with minimal exogenous signals and partial conversion to the naive state Scientific Reports, 2022;12(1):17381. 2022 [PMID: 36253431] (Cell Culture, Human) Cell Culture Human
T Patel, J Hammelman, S Aziz, S Jang, M Closser, TL Michaels, JA Blum, DK Gifford, H Wichterle Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro Nature Communications, 2022;13(1):5427. 2022 [PMID: 36109497] (Cell Culture, Mouse) Cell Culture Mouse
MK Eltahir, M Nakamori, S Hattori, T Kimura, H Mochizuki, S Nagano Pharmacotherapy alleviates pathological changes in human direct reprogrammed neuronal cell model of myotonic dystrophy type 1 PLoS ONE, 2022;17(7):e0269683. 2022 [PMID: 35776705] (Bioassay, Human) Bioassay Human
M Lindkvist, MM Zegeye, M Grenegård, LU Ljungberg Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells International Journal of Molecular Sciences, 2022;23(3):. 2022 [PMID: 35163371] (Bioassay, Human) Bioassay Human
WM Seo, J Yoon, JH Lee, Y Lee, H Lee, D Geum, W Sun, MR Song Modeling axonal regeneration by changing cytoskeletal dynamics in stem cell-derived motor nerve organoids Scientific Reports, 2022;12(1):2082. 2022 [PMID: 35136073] (Bioassay, Human) Bioassay Human
Y Sonobe, J Aburas, G Krishnan, AC Fleming, G Ghadge, P Islam, EC Warren, Y Gu, MW Kankel, AEX Brown, E Kiskinis, TF Gendron, FB Gao, RP Roos, P Kratsios A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation Nature Communications, 2021;12(1):6025. 2021 [PMID: 34654821] (Bioassay, Human) Bioassay Human
C Lin, Å Ekblad-Nor, J Michaëlsso, C Götherströ, CC Hsu, H Ye, J Johansson, A Rising, E Sundström, E Åkesson In Vitro Study of Human Immune Responses to Hyaluronic Acid Hydrogels, Recombinant Spidroins and Human Neural Progenitor Cells of Relevance to Spinal Cord Injury Repair Cells, 2021;10(7):. 2021 [PMID: 34359882] (Bioassay, Human) Bioassay Human
PY Shih, M Kreir, D Kumar, F Seibt, F Pestana, B Schmid, B Holst, C Clausen, R Steeg, B Fischer, J Pita-Almen, A Ebneth, A Cabrera-So Development of a fully human assay combining NGN2-inducible neurons co-cultured with iPSC-derived astrocytes amenable for electrophysiological studies Stem Cell Research, 2021;54(0):102386. 2021 [PMID: 34229210] (Cell Culture, Human) Cell Culture Human
K Russ, G Teku, L Bousset, V Redeker, S Piel, E Savchenko, Y Pomeshchik, J Savistchen, TC Stummann, C Azevedo, A Collin, S Goldwurm, K Fog, E Elmer, M Vihinen, R Melki, L Roybon TNF-&amp;alpha and &amp;alpha-synuclein fibrils differently regulate human astrocyte immune reactivity and impair mitochondrial respiration Cell Reports, 2021;34(12):108895. 2021 [PMID: 33761362] (Bioassay, Human) Bioassay Human
D Hagiwara, M Tochiya, Y Azuma, T Tsumura, Y Hodai, Y Kawaguchi, T Miyata, T Kobayashi, M Sugiyama, T Onoue, H Takagi, Y Ito, S Iwama, H Suga, R Banno, H Arima Arginine vasopressin-Venus reporter mice as a tool for studying magnocellular arginine vasopressin neurons Peptides, 2021;139(0):170517. 2021 [PMID: 33647312] (Bioassay, Mouse) Bioassay Mouse
Show All 44 Publications.

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Bioinformatics

Gene Symbol CNTF
Uniprot