Recombinant Human CNTF Protein, CF

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1 μg/lane of Recombinant Human CNTF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 26 kDa.
Recombinant Human CNTF (Catalog #257‑NT/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.03-0.18 μg/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CNTF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.03-0.18 μg/mL.
The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR) . Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED50 for this effect is 1-3 ng/mL.
Source
E. coli-derived human CNTF protein
Ala2-Met200
Accession #
N-terminal Sequence
Ala2
Structure / Form

This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2-22.8 kDa.

Protein/Peptide Type
Recombinant Proteins
Gene
CNTF
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23-26 kDa, reducing conditions
Publications
Read Publications using
257-NT/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CNTF Protein, CF

  • ciliary neurotrophic factor
  • CNTF
  • HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

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Publications for CNTF (257-NT/CF)(20)

We have publications tested in 3 confirmed species: Human, Canine, Chicken.

We have publications tested in 4 applications: Binding Study, Bioassay, ELISA capture, In Vivo.


Filter By Application
Binding Study
(1)
Bioassay
(15)
ELISA capture
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(14)
Canine
(1)
Chicken
(5)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 257-NT/CF Applications Species
N Rodriguez-, A Parkhitko, T Grass, RM Gibbs, EM Norabuena, N Perrimon, R Singh, LL Rubin Blocking p62/SQSTM1-dependent SMN degradation ameliorates Spinal Muscular Atrophy disease phenotypes J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29672276] (Bioassay, Human) Bioassay Human
MJ Ziller, JA Ortega, KA Quinlan, DP Santos, H Gu, EJ Martin, C Galonska, R Pop, S Maidl, A Di Pardo, M Huang, HY Meltzer, A Gnirke, CJ Heckman, A Meissner, E Kiskinis Dissecting the Functional Consequences of De Novo DNA Methylation Dynamics in Human Motor Neuron Differentiation and Physiology Cell Stem Cell, 2018;0(0):. 2018 [PMID: 29551301] (Bioassay, Human) Bioassay Human
C Coste, V Neirinckx, A Sharma, G Agirman, B Rogister, J Foguenne, F Lallemend, A Gothot, S Wislet Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues PLoS ONE, 2017;12(7):e0177962. 2017 [PMID: 28683107] (Bioassay, Human) Bioassay Human
PH Vincent, E Benedikz, P Uhlen, O Hovatta, E Sundström Expression of Pluripotency Markers in Non-pluripotent Human Neural Stem and Progenitor Cells Stem Cells Dev., 2017;0(0):. 2017 [PMID: 28351210] (Bioassay, Human) Bioassay Human
Q Wang, Y Liu, C Zhang, F Guo, C Feng, X Li, H Shi, Z Su High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration Protein Expr. Purif, 2017;133(0):152-159. 2017 [PMID: 28323167] (Human) Human
Merkle F, Maroof A, Wataya T, Sasai Y, Studer L, Eggan K, Schier A Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells. Development, 2015;142(4):633-43. 2015 [PMID: 25670790] (Bioassay, Human) Bioassay Human
Devlin, Anna-Cla, Burr, Karen, Borooah, Shyamang, Foster, Joshua D, Cleary, Elaine M, Geti, Imbisaat, Vallier, Ludovic, Shaw, Christop, Chandran, Siddhart, Miles, Gareth B Human iPSC-derived motoneurons harbouring TARDBP or C9ORF72 ALS mutations are dysfunctional despite maintaining viability. Nat Commun, 2015;6(0):5999. 2015 [PMID: 25580746] (Bioassay, Human) Bioassay Human
Shi L, Ko M, Huang C, Park S, Hong M, Wu C, Ko G Chicken embryos as a potential new model for early onset type I diabetes. J Diabetes Res, 2014;2014(0):354094. 2014 [PMID: 25133191] (Bioassay, Chicken) Bioassay Chicken
Barmada, Sami J, Serio, Andrea, Arjun, Arpana, Bilican, Bilada, Daub, Aaron, Ando, D Michae, Tsvetkov, Andrey, Pleiss, Michael, Li, Xingli, Peisach, Daniel, Shaw, Christop, Chandran, Siddhart, Finkbeiner, Steven Autophagy induction enhances TDP43 turnover and survival in neuronal ALS models. Nat Chem Biol, 2014;10(8):677-85. 2014 [PMID: 24974230] (Bioassay, Human) Bioassay Human
Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H Involvement of ER stress in dysmyelination of Pelizaeus-Merzbacher Disease with PLP1 missense mutations shown by iPSC-derived oligodendrocytes. Stem Cell Reports, 2014;2(5):648-61. 2014 [PMID: 24936452] (Bioassay, Human) Bioassay Human
Show All 20 Publications.

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Bioinformatics

Gene Symbol CNTF
Entrez
Uniprot