Recombinant Human CNTF Protein, CF

Images

 
1 μg/lane of Recombinant Human CNTF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 26 kDa.
Recombinant Human CNTF (Catalog #257‑NT/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.03-0.18 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CNTF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.03-0.18 μg/mL.
The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR) .
Source
E. coli-derived human CNTF protein
Ala2-Met200
Accession #
N-terminal Sequence
Ala2
Structure / Form

This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2-22.8 kDa.

Protein/Peptide Type
Recombinant Proteins
Gene
CNTF
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23-26 kDa, reducing conditions
Publications
Read Publications using
257-NT/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CNTF Protein, CF

  • ciliary neurotrophic factor
  • CNTF
  • HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

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Publications for CNTF (257-NT/CF)(34)

We have publications tested in 5 confirmed species: Human, Mouse, Canine, Chicken, Mousee.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), In Vivo.


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(25)
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(4)
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Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using 257-NT/CF Applications Species
PY Shih, M Kreir, D Kumar, F Seibt, F Pestana, B Schmid, B Holst, C Clausen, R Steeg, B Fischer, J Pita-Almen, A Ebneth, A Cabrera-So Development of a fully human assay combining NGN2-inducible neurons co-cultured with iPSC-derived astrocytes amenable for electrophysiological studies Stem Cell Research, 2021;54(0):102386. 2021 [PMID: 34229210] (Cell Culture, Human) Cell Culture Human
K Russ, G Teku, L Bousset, V Redeker, S Piel, E Savchenko, Y Pomeshchik, J Savistchen, TC Stummann, C Azevedo, A Collin, S Goldwurm, K Fog, E Elmer, M Vihinen, R Melki, L Roybon TNF-&amp;alpha and &amp;alpha-synuclein fibrils differently regulate human astrocyte immune reactivity and impair mitochondrial respiration Cell Reports, 2021;34(12):108895. 2021 [PMID: 33761362] (Bioassay, Human) Bioassay Human
D Hagiwara, M Tochiya, Y Azuma, T Tsumura, Y Hodai, Y Kawaguchi, T Miyata, T Kobayashi, M Sugiyama, T Onoue, H Takagi, Y Ito, S Iwama, H Suga, R Banno, H Arima Arginine vasopressin-Venus reporter mice as a tool for studying magnocellular arginine vasopressin neurons Peptides, 2021;139(0):170517. 2021 [PMID: 33647312] (Bioassay, Mouse) Bioassay Mouse
E Solomon, K Davis-Ande, B Hovde, S Micheva-Vi, JF Harris, S Twary, R Iyer Global transcriptome profile of the developmental principles of in vitro iPSC-to-motor neuron differentiation BMC molecular and cell biology, 2021;22(1):13. 2021 [PMID: 33602141] (Bioassay, Human) Bioassay Human
V Mashanov, A Alwan, MW Kim, D Lai, A Poerio, YM Ju, JH Kim, JJ Yoo Synergistic effect of CNTF and GDNF on directed neurite growth in chick embryo dorsal root ganglia PLoS ONE, 2020;15(10):e0240235. 2020 [PMID: 33017447] (Bioassay, Chicken) Bioassay Chicken
Y Men, L Ye, RD Risgaard, V Promes, X Zhao, M Paukert, Y Yang Astroglial FMRP deficiency cell-autonomously up-regulates miR-128 and disrupts developmental astroglial mGluR5 signaling Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):. 2020 [PMID: 32958647] (Bioassay, Human) Bioassay Human
KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri Modulation of Human Adipose Stem Cells&#039; Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 2020;9(9):. 2020 [PMID: 32839392] (Cell Culture, Human) Cell Culture Human
M Grosch, S Ittermann, E Rusha, T Greisle, C Ori, DJ Truong, AC O'Neill, A Pertek, GG Westmeyer, M Drukker Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation BMC Biol., 2020;18(1):42. 2020 [PMID: 32321486] (Cell Culture, Human) Cell Culture Human
M Chumarina, K Russ, C Azevedo, A Heuer, M Pihl, A Collin, EÅ Frostner, E Elmer, P Hyttel, G Cappellett, M Zini, S Goldwurm, L Roybon Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene Acta Neuropathol Commun, 2019;7(1):208. 2019 [PMID: 31843010] (Bioassay, Human) Bioassay Human
JC Butts, N Iyer, N White, R Thompson, S Sakiyama-E, TC McDevitt V2a interneuron differentiation from mouse and human pluripotent stem cells Nat Protoc, 2019;0(0):. 2019 [PMID: 31628445] (Bioassay, Human) Bioassay Human
Show All 34 Publications.

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Bioinformatics

Gene Symbol CNTF
Uniprot