Recombinant Human CD31/PECAM-1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD31/PECAM-1 Protein, CF Summary

• Details of Functionality: Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5 x 10 4 cells/well are added to rhCD31 coated plates (3.3 μg/mL, 100 μL/well) in the presence of 20 ng.mL PMA, approximately 50-65% will adhere after 30 minutes at 37degreesC.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CD31/PECAM-1 protein Gln28-Lys601
• Accession #: P16284
• N-terminal Sequence: No results obtained: Gln28 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: PECAM1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 64.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 150 μg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD31/PECAM-1 Protein, CF

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Publications for CD31/PECAM-1 (ADP6)(2)

Publications using ADP6

• Spleen Tyrosine Kinase Is Involved in the CD38 Signal Transduction Pathway in Chronic Lymphocytic Leukemia PLoS ONE, 2016-12-30;11(12):e0169159. 2016-12-30 [PMID: 28036404] (Bioassay, Human)
• Wei H, Song J, Fang L, Li G, Chatterjee S Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+ mobilization. Biochem. Biophys. Res. Commun., 2004-08-06;320(4):1228-35. 2004-08-06 [PMID: 15249221] (Western Blot)

Bioinformatics

• Gene Symbol: PECAM1
• Uniprot:
  • P16284()