Recombinant Human CCL5/RANTES Protein, CF

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1 µg/lane of Recombinant Human CCL5/RANTES was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.
Recombinant Human CCL5/RANTES (Catalog # 278-RN/CF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5. The ED50 for this effect is 1‑5 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CCL5/RANTES Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 1‑5 ng/mL.
Source
E. coli-derived human CCL5/RANTES protein
Ser24-Ser91
Accession #
N-terminal Sequence
Ser24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL5
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
278-RN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL5/RANTES Protein, CF

  • CCL5
  • chemokine (C-C motif) ligand 5
  • D17S136Enormally T-expressed, and presumably secreted
  • EoCP
  • Eosinophil chemotactic cytokine
  • RANTES
  • SISd
  • SIS-delta
  • small inducible cytokine A5 (RANTES)
  • small inducible cytokine subfamily A (Cys-Cys), member 5
  • Small-inducible cytokine A5
  • T cell-specific protein P228
  • T-cell specific protein p288
  • TCP228T-cell-specific protein RANTES

Background

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature human CCL5 shares 75%-84% aa seqeuence identity with canine, cotton rat, feline, mouse, and rat CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11-13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14-16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

  1. Schall, T.J. et al. (1990) Nature 347:669.
  2. Bacon, K.B. et al. (1995) Science 269:1727.
  3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
  4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
  5. Schall, T.J. et al. (1988) J. Immunol. 141:1018.
  6. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
  7. Levy, J.A. (2009) J. Immunol. 182:3945.
  8. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
  9. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
  10. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  11. Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
  12. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885.
  13. von Hundelshausen, P. et al. (2005) Blood 105:924.
  14. von Hundelshausen, P. et al. (2001) Circulation 103:1772.
  15. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
  16. Baltus, T. et al. (2003) Blood 102:1985.
  17. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.

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Publications for CCL5/RANTES (278-RN/CF)(49)

We have publications tested in 5 confirmed species: Human, Rat, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA (Standard), Hemofiltration Procedure.


Filter By Application
Binding Assay
(3)
Bioassay
(34)
ELISA (Standard)
(11)
Hemofiltration Procedure
(1)
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Filter By Species
Human
(33)
Rat
(2)
N/A
(11)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Virus
(1)
All Species
Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using 278-RN/CF Applications Species
G An, F Wu, S Huang, L Feng, J Bai, S Gu, X Zhao Effects of CCL5 on the biological behavior of breast cancer and the mechanisms of its interaction with tumor?associated macrophages Oncol. Rep., 2019;0(0):. 2019 [PMID: 31578575] (Bioassay, Human) Bioassay Human
G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5 Cell Death Dis, 2019;10(4):264. 2019 [PMID: 30890699] (Bioassay, Human) Bioassay Human
Y Nam, SM Jung, YA Rim, H Jung, K Lee, N Park, J Kim, Y Jang, YB Park, SH Park, JH Ju Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis PLoS ONE, 2018;13(6):e0198740. 2018 [PMID: 29879214] (Bioassay, Human) Bioassay Human
YC Wu, YC Shen, JW Chang, JJ Hsieh, Y Chu, CH Wang Autocrine CCL5 promotes tumor progression in esophageal squamous cell carcinoma in vitro Cytokine, 2018;110(0):94-103. 2018 [PMID: 29705397] (Bioassay, Human) Bioassay Human
HJ Cha, HY Kim, HS Kim Sulfatase 1 mediates the attenuation of Ang II-induced hypertensive effects by CCL5 in vascular smooth muscle cells from spontaneously hypertensive rats Cytokine, 2018;110(0):1-8. 2018 [PMID: 29684635] (Bioassay, Rat) Bioassay Rat
JG Chen, X Liu, M Munshi, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, GG Chan, CJ Patterson, KE Meid, J Gustine, T Dubeau, P Severns, JJ Castillo, ZR Hunter, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism Blood, 2018;0(0):. 2018 [PMID: 29496671] (Bioassay, Human) Bioassay Human
S Urata, K Izumi, K Hiratsuka, A Maolake, A Natsagdorj, K Shigehara, H Iwamoto, S Kadomoto, T Makino, R Naito, Y Kadono, WJ Lin, G Wufuer, K Narimoto, A Mizokami C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment Cancer Sci., 2018;109(3):724-731. 2018 [PMID: 29288523] (Bioassay, Human) Bioassay Human
A Venuti, C Pastori, G Siracusano, R Pennisi, A Riva, M Tommasino, MT Sciortino, L Lopalco The Abrogation of Phosphorylation Plays a Relevant Role in the CCR5 Signalosome Formation with Natural Antibodies to CCR5 Viruses, 2017;10(1):. 2017 [PMID: 29283386] (Bioassay, Human) Bioassay Human
D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017;7(1):8536. 2017 [PMID: 28819198] (Bioassay, Human) Bioassay Human
Y Yang, S Hu, J Liu, Y Cui, Y Fan, T Lv, L Liu, J Li, Q He, W Han, W Yu, Y Sun, J Jin CD8+ T cells promote proliferation of benign prostatic hyperplasia epithelial cells under low androgen level via modulation of CCL5/STAT5/CCND1 signaling pathway Sci Rep, 2017;7(0):42893. 2017 [PMID: 28216616] (Bioassay, Human) Bioassay Human
Show All 49 Publications.

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Bioinformatics

Gene Symbol CCL5
Uniprot