CCL5/RANTES Antibodies

278-RN) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) for this effect is typically 80.0-800 ng/mL in the presence of 10 ng/mL Recombinant Human CCL5/RANTES." class="big_lightbox"> 278-RN) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) for this effect is typically 80.0-800 ng/mL in the presence of 10 ng/mL Recombinant Human CCL5/RANTES." class="big_thumb" />

278-RN) was serially diluted and captured by Mouse Anti-Human/Primate CCL5/RANTES Monoclonal Antibody (Catalog # MAB678) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 278-RN) was serially diluted and captured by Mouse Anti-Human/Primate CCL5/RANTES Monoclonal Antibody (Catalog # MAB678) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

478-MR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.025 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL5/ RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478). The ND₅₀ is typically 0.07-0.3 µg/mL." class="big_lightbox"> 478-MR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.025 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL5/ RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478). The ND₅₀ is typically 0.07-0.3 µg/mL." alt="Recombinant Mouse CCL5/RANTES (478-MR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.025 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL5/ RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478). The ND₅₀ is typically 0.07-0.3 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

478-MR) was serially diluted and captured by Rat Anti-Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB4781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 478-MR) was serially diluted and captured by Rat Anti-Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB4781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

278-RN), Recombinant Mouse CCL5/RANTES (478-MR), Recombinant Human CCL3/MIP-1a Isoform LD78a (270-LD), and Recombinant Human CCL4/MIP-1 beta (271-BME). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CCL5/RANTES at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 278-RN), Recombinant Mouse CCL5/RANTES (478-MR), Recombinant Human CCL3/MIP-1a Isoform LD78a (270-LD), and Recombinant Human CCL4/MIP-1 beta (271-BME). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CCL5/RANTES at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human CCL5/RANTES (278-RN), Recombinant Mouse CCL5/RANTES (478-MR), Recombinant Human CCL3/MIP-1a Isoform LD78a (270-LD), and Recombinant Human CCL4/MIP-1 beta (271-BME). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CCL5/RANTES at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_thumb" />

278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA). The ND₅₀ is typically 0.1‑0.4 µg/mL." class="big_lightbox"> 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA). The ND₅₀ is typically 0.1‑0.4 µg/mL." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

278-RN) was serially diluted and captured by Mouse Anti-Human/Primate CCL5/RANTES Monoclonal Antibody (Catalog # MAB678) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 278-RN) was serially diluted and captured by Mouse Anti-Human/Primate CCL5/RANTES Monoclonal Antibody (Catalog # MAB678) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CCL5/RANTES was detected in immersion fixed mouse splenocytes using Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

478-MR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.025 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_lightbox"> 478-MR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.025 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_thumb" />

278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox"> 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

478-MR) was serially diluted and captured by Rat Anti-Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB4781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 478-MR) was serially diluted and captured by Rat Anti-Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB4781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse CCL5/RANTES (Catalog # 478-MR) was serially diluted and captured by Rat Anti-Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB4781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF478) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

278-RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB2781). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_lightbox"> 278-RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB2781). The ND₅₀ is typically 0.2-0.8 µg/mL." alt="Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB2781). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_thumb" />

3819-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline CCL5/ RANTES (80 ng/mL) is neutral-ized (green line) by increasing concen-trations of Sheep Anti-Feline CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3819). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_lightbox"> 3819-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline CCL5/ RANTES (80 ng/mL) is neutral-ized (green line) by increasing concen-trations of Sheep Anti-Feline CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3819). The ND₅₀ is typically 0.5-2.5 µg/mL." alt="Recombinant Feline CCL5/ RANTES (Catalog # 3819-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline CCL5/ RANTES (80 ng/mL) is neutral-ized (green line) by increasing concen-trations of Sheep Anti-Feline CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3819). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_thumb" />

278-RN) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through to the membrane was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL5/ RANTES (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Polyclonal Antibody (Catalog # AB-278-NA). The ND₅₀ is typically 10-30 µg/mL." class="big_lightbox"> 278-RN) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through to the membrane was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL5/ RANTES (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Polyclonal Antibody (Catalog # AB-278-NA). The ND₅₀ is typically 10-30 µg/mL." alt="Recombinant Human CCL5/ RANTES (Catalog # 278-RN) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through to the membrane was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL5/ RANTES (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Polyclonal Antibody (Catalog # AB-278-NA). The ND₅₀ is typically 10-30 µg/mL." class="big_thumb" />

1010-CR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL5/RANTES (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1010). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 1010-CR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL5/RANTES (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1010). The ND₅₀ is typically 1-4 µg/mL." alt="Recombinant Cotton Rat CCL5/RANTES (Catalog # 1010-CR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL5/RANTES (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1010). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CCL5/RANTES was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Canine CCL5/RANTES Monoclonal Antibody (Catalog # MAB60451) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />