Recombinant Human CCL19/MIP-3 beta Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CCL19/MIP-3 beta Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.1-0.3 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 3-15 ng/mL.
Source
E. coli-derived human CCL19/MIP-3 beta protein
Gly22-Ser98
Accession #
N-terminal Sequence
Gly22
Protein/Peptide Type
Recombinant Proteins
Gene
CCL19
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
361-MI/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL19/MIP-3 beta Protein, CF

  • beta chemokine exodus-3
  • Beta-chemokine exodus-3
  • CC chemokine ligand 19
  • C-C motif chemokine 19
  • CCL19
  • chemokine (C-C motif) ligand 19
  • CKb11
  • EBI1-ligand chemokine
  • ELC
  • ELCMIP-3-beta
  • Epstein-Barr virus-induced molecule 1 ligand chemokine
  • exodus-3
  • Macrophage inflammatory protein 3 beta
  • macrophage inflammatory protein 3-beta
  • MGC34433
  • MIP3 beta
  • MIP-3 beta
  • MIP-3b
  • MIP3BCK beta-11
  • SCYA19EBI1 ligand chemokine
  • small inducible cytokine subfamily A (Cys-Cys), member 19
  • Small-inducible cytokine A19

Background

MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is one of many novel beta chemokines identified through bioinformatics. MIP-3 beta cDNA encodes a 98 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is cleaved to form the 77 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity) and the gene for MIP-3 beta  has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for cultured human lymphocytes. MIP-3 beta has also been shown to be a unique functional ligand for CCR-7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. EBI1 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

  1. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

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Publications for CCL19/MIP-3 beta (361-MI/CF)(28)

We have publications tested in 3 confirmed species: Human, N/A, Virus.

We have publications tested in 3 applications: Binding Assay, Bioassay, ELISA Standard.


Filter By Application
Binding Assay
(1)
Bioassay
(26)
ELISA Standard
(1)
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Human
(26)
N/A
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 361-MI/CF Applications Species
H Plešingero, P Janovská, A Mishra, L Smy?ková, L Poppová, A Libra, K Plevová, P Ovesná, L Radová, M Doubek, Š Pavlová, Š Pospíšilov, V Bryja Expression of COBLL1 encoding novel ROR1 binding partner is robust predictor of survival in chronic lymphocytic leukemia Haematologica, 2017;0(0):. 2017 [PMID: 29122990] (Bioassay, Human) Bioassay Human
D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017;7(1):8536. 2017 [PMID: 28819198] (Bioassay, Human) Bioassay Human
JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use J Immunol Res, 2017;2017(0):1975902. 2017 [PMID: 28785596] (Bioassay, Human) Bioassay Human
B Xu, M Zhou, W Qiu, J Ye, Q Feng CCR7 mediates human breast cancer cell invasion, migration by inducing epithelial-mesenchymal transition and suppressing apoptosis through AKT pathway Cancer Med, 2017;0(0):. 2017 [PMID: 28378417] (Bioassay, Human) Bioassay Human
RA Raposo, M de Mulder, D Paquin-Pro, PM Brailey, VD Cabido, PM Zdinak, AS Thomas, SH Huang, GA Beckerle, RB Jones, DF Nixon IFITM1 targets HIV-1 latently infected cells for antibody-dependent cytolysis JCI Insight, 2017;2(1):e85811. 2017 [PMID: 28097226] (Bioassay, Human) Bioassay Human
Dengue virus infection induces interferon-lambda1 to facilitate cell migration Sci Rep, 2016;6(0):24530. 2016 [PMID: 27456172] (Bioassay, Human) Bioassay Human
Human breast cancer-derived soluble factors facilitate CCL19-induced chemotaxis of human dendritic cells Sci Rep, 2016;6(0):30207. 2016 [PMID: 27451948] (Bioassay, Human) Bioassay Human
RB Jones, S Mueller, R O'Connor, K Rimpel, DD Sloan, D Karel, HC Wong, EK Jeng, AS Thomas, JB Whitney, SY Lim, C Kovacs, E Benko, S Karandish, SH Huang, MJ Buzon, M Lichterfel, A Irrinki, JP Murry, A Tsai, H Yu, R Geleziunas, A Trocha, MA Ostrowski, Irvi A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes PLoS Pathog, 2016;12(4):e1005545. 2016 [PMID: 27082643] (Bioassay, Human) Bioassay Human
Janovska P, Poppova L, Plevova K, Plesingerova H, Behal M, Kaucka M, Ovesna P, Hlozkova M, Borsky M, Stehlikova O, Brychtova Y, Doubek M, Machalova M, Baskar S, Kozubik A, Pospisilova S, Pavlova S, Bryja V Autocrine Signaling by Wnt-5a Deregulates Chemotaxis of Leukemic Cells and Predicts Clinical Outcome in Chronic Lymphocytic Leukemia. Clin Cancer Res, 2016;22(2):459-69. 2016 [PMID: 26240275] (Bioassay, Human) Bioassay Human
Kaucka M, Petersen J, Janovska P, Radaszkiewicz T, Smyckova L, Daulat A, Borg J, Schulte G, Bryja V Asymmetry of VANGL2 in migrating lymphocytes as a tool to monitor activity of the mammalian WNT/planar cell polarity pathway. Cell Commun Signal, 2015;13(0):2. 2015 [PMID: 25627785] (Bioassay, Human) Bioassay Human
Show All 28 Publications.

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Bioinformatics

Gene Symbol CCL19
Entrez
Uniprot