Recombinant Human CCL16/HCC-4 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CCL16/HCC-4 Protein Summary

Details of Functionality
Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. Hedrick, J. et al. (1998) Blood 91:4242. The ED50 for this effect is 0.5‑2 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 0.15‑0.75 µg/mL.
Source
E. coli-derived human CCL16/HCC-4 protein
Gln24-Gln120
Accession #
N-terminal Sequence
Gln24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL16
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
802-HC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL16/HCC-4 Protein

  • C-C motif chemokine 16
  • CCL16
  • chemokine (C-C motif) ligand 16
  • Chemokine CC-4
  • Chemokine LEC
  • CKb12
  • HCC4
  • HCC-4
  • HCC-4Liver-expressed chemokine
  • LCC-1Lymphocyte and monocyte chemoattractant
  • LEC
  • liver CC chemokine-1
  • LMC
  • LMCSmall-inducible cytokine A16
  • monotactin-1
  • Mtn-1
  • NCC-4
  • NCC4IL-10-inducible chemokine
  • NCC-4ILINCK
  • new CC chemokine 4
  • SCYA16MGC117051
  • SCYL4
  • small inducible cytokine subfamily A (Cys-Cys), member 16

Background

Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered.

Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells.

  1. Shoudai, K. et al. (1998) Biochim. Biophys. Acta 1396:273.
  2. Hedrick, J. et al. (1998) Blood 91:4242.
  3. Youn, B-S. et al. (1998) BBRC 247:217.

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Publications for CCL16/HCC-4/LEC (802-HC)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Virus.

We have publications tested in 2 applications: Binding Assay, Bioassay.


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Binding Assay
(1)
Bioassay
(4)
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Human
(3)
Mouse
(1)
Virus
(1)
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Bioinformatics

Gene Symbol CCL16
Uniprot