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Recombinant Human BMP-4 Protein, CF


1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. more
Recombinant Human BMP-4 (Catalog #314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 2.5-15 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-4 Protein, CF Summary

Details of Functionality
Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.
Mouse myeloma cell line, NS0-derived human BMP-4 protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
  • Bioactivity2
Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
19-24 kDa, reducing conditions
35-41 kDa, non-reducing conditions
Read Publications using
314-BP/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50-200 μg/mL in sterile 4 mM HCl.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-4 Protein, CF

  • BMP2B
  • BMP-2B
  • BMP2B1
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • MCOPS6
  • OFC11
  • ZYME


BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7
disulfide-linked heterodimer (7-9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).


  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Wozney, J. et al. (1988) Science 242:1528.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

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Publications for BMP-4 (314-BP/CF)(427)

We have publications tested in 15 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Equine, Northern White Rhinoceros, Ovine, Porcine, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Rabbit, Xenopus, Zebrafish.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, In Ovo, In Vivo, Surface Plasmon Resonance, Tissue Culture, Western Blot.

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Binding Assay
Cell Culture
In Ovo
In Vivo
Surface Plasmon Resonance
Tissue Culture
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All Applications
Filter By Species
Northern White Rhinoceros
Primate - Callitrix jacchus (Common Marmoset)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
All Species
Showing Publications 1 - 10 of 427. Show All 427 Publications.
Publications using 314-BP/CF Applications Species
Z Wang, HP McWilliams, H Reza, JR Ostberg, W Chen, X Wang, C Huynh, V Vyas, WC Chang, R Starr, JR Wagner, B Aguilar, X Yang, X Wu, J Wang, W Chen, E Koelker-Wo, CS Seet, A Montel-Hag, GM Crooks, SJ Forman, CE Brown 3D-organoid culture supports differentiation of human CAR+ iPSCs into highly functional CAR T�cells Cell Stem Cell, 2022;0(0):. 2022 [PMID: 35278370] (Bioassay, Human) Bioassay Human
V Zywitza, E Rusha, D Shaposhnik, J Ruiz-Orera, N Telugu, V Rishko, M Hayashi, G Michel, L Wittler, J Stejskal, S Holtze, F Göritz, R Hermes, J Wang, Z Izsvák, S Colleoni, G Lazzari, C Galli, TB Hildebrand, K Hayashi, S Diecke, M Drukker Na�ve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction Scientific Reports, 2022;12(1):3100. 2022 [PMID: 35260583] (Bioassay, Northern White Rhinoceros) Bioassay Northern White Rhinoceros
K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids Stem Cell Reports, 2022;0(0):. 2022 [PMID: 35245440] (Bioassay, Human) Bioassay Human
X Hu, C Lin, N Ruan, Z Huang, Y Zhang, X Hu Operation of the Atypical Canonical Bone Morphogenetic Protein Signaling Pathway During Early Human Odontogenesis Frontiers in Physiology, 2022;13(0):823275. 2022 [PMID: 35211032] (Cell Culture, Human) Cell Culture Human
MC Ribeiro, JM Rivera-Arb, C Cofiño-Fab, V Schwach, RH Slaats, SA Ten Den, K Vermeul, A van den Be, JM Pérez-Poma, LI Segerink, JA Guadix, R Passier A New Versatile Platform for Assessment of Improved Cardiac Performance in Human-Engineered Heart Tissues Journal of personalized medicine, 2022;12(2):. 2022 [PMID: 35207702] (Bioassay, Human) Bioassay Human
JJ Kathiriya, C Wang, M Zhou, A Brumwell, M Cassandras, CJ Le Saux, M Cohen, KD Alysandrat, B Wang, P Wolters, M Matthay, DN Kotton, HA Chapman, T Peng Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells Nature Cell Biology, 2021;0(0):. 2021 [PMID: 34969962] (Bioassay, Human) Bioassay Human
N Yucer, R Ahdoot, MJ Workman, AH Laperle, MS Recouvreux, K Kurowski, DJ Naboulsi, V Liang, Y Qu, JT Plummer, SA Gayther, S Orsulic, BY Karlan, CN Svendsen Human iPSC-derived fallopian tube organoids with BRCA1 mutation recapitulate early-stage carcinogenesis Cell Reports, 2021;37(13):110146. 2021 [PMID: 34965417] (Bioassay, Human) Bioassay Human
AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34856121] (Bioassay, Human) Bioassay Human
F Xu, C Deng, Z Ren, L Sun, Y Meng, W Liu, J Wan, G Chen Lysophosphatidic acid shifts metabolic and transcriptional landscapes to induce a distinct cellular state in human pluripotent stem cells Cell Reports, 2021;37(9):110063. 2021 [PMID: 34852227] (Bioassay, Human) Bioassay Human
AF de Souza, FF Bressan, NCG Pieri, RC Botigelli, T Revay, SK Haddad, DT Covas, ES Ramos, WA King, FV Meirelles Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients Cells, 2021;10(11):. 2021 [PMID: 34831322] (Bioassay, Human) Bioassay Human
Show All 427 Publications.

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Gene Symbol BMP4