Recombinant Human BMP-4 Protein, CF


Recombinant Human BMP‑4 (Catalog # 314-BP/CF) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL.
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. more

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Applications Bioactivity

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Recombinant Human BMP-4 Protein, CF Summary

Details of Functionality
Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.
Mouse myeloma cell line, NS0-derived human BMP-4 protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
  • Bioactivity2
Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
19-24 kDa, reducing conditions
35-41 kDa, non-reducing conditions
Read Publications using
314-BP/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50-200 μg/mL in sterile 4 mM HCl.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-4 Protein, CF

  • BMP2B
  • BMP-2B
  • BMP2B1
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • MCOPS6
  • OFC11
  • ZYME


BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7
disulfide-linked heterodimer (7-9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).


  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Wozney, J. et al. (1988) Science 242:1528.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

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Publications for BMP-4 (314-BP/CF)(480)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Equine, Northern White Rhinoceros, Ovine, Porcine, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Rabbit, Rhinoceros, Xenopus, Zebrafish.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, In Ovo, In Vivo, Surface Plasmon Resonance, Tissue Culture, Western Blot.

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Binding Assay
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In Ovo
In Vivo
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Filter By Species
Northern White Rhinoceros
Primate - Callitrix jacchus (Common Marmoset)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
All Species
Showing Publications 1 - 10 of 480. Show All 480 Publications.
Publications using 314-BP/CF Applications Species
Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung&#039;s disease Cell Stem Cell, 2023;30(3):264-282.e9. 2023 [PMID: 36868194] (Bioassay, Human) Bioassay Human
M Shono, K Kishimoto, O Hikabe, M Hayashi, K Semi, Y Takashima, E Sasaki, K Kato, K Hayashi Induction of primordial germ cell-like cells from common marmoset embryonic stem cells by inhibition of WNT and retinoic acid signaling Scientific Reports, 2023;13(1):3186. 2023 [PMID: 36823310] (Bioassay, Human) Bioassay Human
EF Cohn, BLL Clayton, M Madhavan, S Yacoub, Y Federov, K Paul-Fried, TJ Shafer, PJ Tesar Pervasive environmental chemicals impair oligodendrocyte development bioRxiv : the preprint server for biology, 2023;0(0):. 2023 [PMID: 36798415] (Bioassay, Mouse) Bioassay Mouse
L Feige, T Kozaki, G Dias de Me, V Guillemot, F Larrous, F Ginhoux, H Bourhy Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses Viruses, 2022;15(1):. 2022 [PMID: 36680128] (Bioassay, Human) Bioassay Human
Q Liu, Z Liu, H Gu, Y Ge, X Wu, F Zuo, Q Du, Y Lei, Z Wang, H Lin Comparative study of differentiating human pluripotent stem cells into vascular smooth muscle cells in hydrogel-based culture methods Regenerative Therapy, 2023;22(0):39-49. 2023 [PMID: 36618488] (Bioassay, Human) Bioassay Human
N Wang, J He, X Feng, S Liao, Y Zhao, F Tang, K Kee Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8 Elife, 2023;12(0):. 2023 [PMID: 36602025] (Bioassay, Human) Bioassay Human
T Oba, S Okamoto, Y Ueno, M Matsuo, T Tadokoro, S Kobayashi, K Yasumura, S Kagimoto, Y Inaba, H Taniguchi In vitro elastic cartilage reconstruction using human auricular perichondrial chondroprogenitor cell-derived micro 3D spheroids Journal of tissue engineering, 2022;13(0):2041731422114. 2022 [PMID: 36582939]
S Yoo, EJ Lee, NX Thang, H La, H Lee, C Park, DW Han, SJ Uhm, H Song, JT Do, Y Choi, K Hong INO80 Is Required for the Cell Cycle Control, Survival, and Differentiation of Mouse ESCs by Transcriptional Regulation International Journal of Molecular Sciences, 2022;23(23):. 2022 [PMID: 36499727] (Bioassay, Mouse) Bioassay Mouse
M Hayashi, V Zywitza, Y Naitou, N Hamazaki, F Goeritz, R Hermes, S Holtze, G Lazzari, C Galli, J Stejskal, S Diecke, TB Hildebrand, K Hayashi Robust induction of primordial germ cells of white rhinoceros on the brink of extinction Science Advances, 2022;8(49):eabp9683. 2022 [PMID: 36490332] (Bioassay, Rhinoceros) Bioassay Rhinoceros
JE Kaserman, RB Werder, F Wang, T Matte, MI Higgins, M Dodge, J Lindstrom-, P Bawa, A Hinds, E Bullitt, IS Caballero, X Shi, RE Gerszten, N Brunetti-P, M Liesa, C Villacorta, AN Hollenberg, DN Kotton, AA Wilson Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity Cell Reports, 2022;41(10):111775. 2022 [PMID: 36476855] (Bioassay, Human) Bioassay Human
Show All 480 Publications.

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Gene Symbol BMP4