BMP-4 Antibodies

314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND₅₀ is typically 1-3 µg/mL in the presence of heparin (2 µg/mL)." class="big_lightbox"> 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND₅₀ is typically 1-3 µg/mL in the presence of heparin (2 µg/mL)." alt="Recombinant Human BMP‑4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND₅₀ is typically 1-3 µg/mL in the presence of heparin (2 µg/mL)." class="big_thumb" />

314-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF757). The ND₅₀ is typically 0.5-2.0 µg/mL in the presence of heparin (2 µg/mL)." class="big_lightbox"> 314-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF757). The ND₅₀ is typically 0.5-2.0 µg/mL in the presence of heparin (2 µg/mL)." alt="Recombinant Human BMP-4 (Catalog # 314-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF757). The ND₅₀ is typically 0.5-2.0 µg/mL in the presence of heparin (2 µg/mL)." class="big_thumb" />

5020-BP) and lysates of mouse embryo tissue. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB5020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for BMP-4 at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 5020-BP) and lysates of mouse embryo tissue. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB5020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for BMP-4 at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows Recombinant Mouse BMP-4 (Catalog # 5020-BP) and lysates of mouse embryo tissue. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB5020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for BMP-4 at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastrointestinal tract cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastrointestinal tract cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

5020-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP-4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB50201). The ND₅₀ is typically 0.4-2.4 µg/mL." class="big_lightbox"> 5020-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP-4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB50201). The ND₅₀ is typically 0.4-2.4 µg/mL." alt="Recombinant Mouse BMP-4 (Catalog # 5020-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP-4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB50201). The ND₅₀ is typically 0.4-2.4 µg/mL." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. " class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. " alt="BMP-4 was detected in immersion fixed HepG2 human hepatocytoma cell line (positive) or K562 human myelogenous leukemia cell line (negative) using Rabbit Anti-Human BMP-4 Monoclonal Antibody (Catalog # MAB7572) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. " class="big_thumb" />