BMP-4 Antibody (66119) [Unconjugated]

Recombinant Human BMP‑4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND50 is typically 1-3 µg/mL in the presence of heparin (2 µg/mL).

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 66119
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

BMP-4 Antibody (66119) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BMP‑4
  Ser293-Arg408
  Accession # P12644
• Specificity: Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP‑2.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMP4
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Neutralization 1-3 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-4 Antibody (66119) [Unconjugated]

• BMP2B
• BMP-2B
• BMP2B1
• BMP2BMCOPS6
• BMP4
• BMP-4
• Bone morphogenetic protein 2B
• bone morphogenetic protein 4
• DVR4
• MCOPS6
• OFC11
• ZYME

Background

BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).

1. Zhang, P. et al., 2008, Blood 111:1933.
2. Gambaro, K. et al., 2006, Cell Death Differ. 13:1075.
3. Simic, P.  and Vukicevic, S., 2005, Cytokine Growth Factor Rev. 16:299.
4. Sadlon, T.J. et al., 2004, Stem Cells 22:457.
5. Frank, D.B. et al., 2005, Circ. Res. 97:496.
6. Wozney, J. et al., 1988, Science 242:1528.
7. Cui, Y. et al., 1998, EMBO J. 17:4735.
8. Cui, Y. et al., 2001, Genes Dev. 15:2797.
9. Aono, A. et al., 1995, Biochem. Biophys. Res. Commun. 210:670.
10. Nishimatsu, S. and G.H. Thomsen, 1998, Mech. Dev. 74:75.
11. Chen, D. et al., 2004, Growth Factors 22:233.
12. Lavery, K. et al., 2008, J. Biol. Chem. 283:20948.
13. Rosen, V., 2006, Ann. N.Y. Acad. Sci. 1068:19.
14. Jones, C.M. and J.C. Smith, 1998, Dev. Biol. 194:12.
15. Takada, T. et al., 2003, J. Biol. Chem. 278:43229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMP-4 Antibody (MAB757)(12)

Publications using MAB757

• L Dupays, N Towers, S Wood, A David, DJ Stuckey, T Mohun Furin, a transcriptional target of NKX2-5, has an essential role in heart development and function PLoS ONE, 2019;14(3):e0212992. 2019 [PMID: 30840660] (Western Blot, Mouse)
• AK Patel, K Vipparthi, V Thatikonda, I Arun, S Bhattachar, R Sharan, P Arun, S Singh A subtype of cancer-associated fibroblasts with lower expression of alpha-smooth muscle actin suppresses stemness through BMP4 in oral carcinoma Oncogenesis, 2018;7(10):78. 2018 [PMID: 30287850] (Neutralization, Human)
• N Blanchette, D Kita, A Konstorum, L Tesfay, D Lemler, P Hegde, KP Claffey, FM Torti, SV Torti Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer Oncogene, 2018;37(29):4013-4032. 2018 [PMID: 29695834] (Neutralization, Human)
• C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28747434] (Immunoprecipitation, Human)
• Herrera B, Inman GJ, A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. BMC Cell Biol., 2009;10(0):20. 2009 [PMID: 19298647] (Neutralization, Human)
• Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling. Blood, 2008;112(8):3154-63. 2008 [PMID: 18664625] (Neutralization, Human)
• Kane R, Godson C, O'Brien C Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis. Mol. Vis., 2008;14(0):1138-48. 2008 [PMID: 18587495] (Western Blot, Human)
• Zode GS, Clark AF, Wordinger RJ Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells. Invest. Ophthalmol. Vis. Sci., 2007;48(11):5058-67. 2007 [PMID: 17962458] (Western Blot, Human)
• Fuller ML, DeChant AK, Rothstein B, Caprariello A, Wang R, Hall AK, Miller RH Bone morphogenetic proteins promote gliosis in demyelinating spinal cord lesions. Ann. Neurol., 2007;62(3):288-300. 2007 [PMID: 17696121] (IHC-Fr, Rat)
• Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach. J. Pathol., 2006;209(2):190-7. 2006 [PMID: 16550632] (IHC-P, Human)
• Moser M, Binder O, Wu Y, Aitsebaomo J, Ren R, Bode C, Bautch VL, Conlon FL, Patterson C BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation. Mol. Cell. Biol., 2003;23(16):5664-79. 2003 [PMID: 12897139] (Neutralization, Human)
• van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP Sonic hedgehog regulates gastric gland morphogenesis in man and mouse. Gastroenterology, 2001;121(2):317-28. 2001 [PMID: 11487541] (IHC-P, Human)

Review for BMP-4 Antibody (MAB757) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 04/04/2020

Summary

• Application: Western Blot
• Sample Tested: breast cancer MCF7 cells,BT-20 human breast cancer cell line
• Species: Human
• Lot: CEO011901B

Bioinformatics

• Gene Symbol: BMP4
• Uniprot:
  • P12644()