BMP-4 Antibody (66119) [Unconjugated]

Images

 
Recombinant Human BMP‑4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clone
66119
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

BMP-4 Antibody (66119) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMP‑4
Ser293-Arg408
Accession # P12644
Specificity
Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP‑2.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
BMP4
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Neutralization 1-3 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB757 in the following applications:

Publications
Read Publications using
MAB757 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-4 Antibody (66119) [Unconjugated]

  • BMP2B
  • BMP-2B
  • BMP2B1
  • BMP2BMCOPS6
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • MCOPS6
  • OFC11
  • ZYME

Background

BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).
  1. Zhang, P. et al., 2008, Blood 111:1933.
  2. Gambaro, K. et al., 2006, Cell Death Differ. 13:1075.
  3. Simic, P.  and Vukicevic, S., 2005, Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al., 2004, Stem Cells 22:457.
  5. Frank, D.B. et al., 2005, Circ. Res. 97:496.
  6. Wozney, J. et al., 1988, Science 242:1528.
  7. Cui, Y. et al., 1998, EMBO J. 17:4735.
  8. Cui, Y. et al., 2001, Genes Dev. 15:2797.
  9. Aono, A. et al., 1995, Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen, 1998, Mech. Dev. 74:75.
  11. Chen, D. et al., 2004, Growth Factors 22:233.
  12. Lavery, K. et al., 2008, J. Biol. Chem. 283:20948.
  13. Rosen, V., 2006, Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith, 1998, Dev. Biol. 194:12.
  15. Takada, T. et al., 2003, J. Biol. Chem. 278:43229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BMP-4 Antibody (MAB757)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: IHC-Fr, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
IHC-Fr
(1)
IHC-P
(2)
Immunoprecipitation
(1)
Neutralization
(5)
Western Blot
(3)
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Filter By Species
Human
(10)
Mouse
(1)
Rat
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB757 Applications Species
L Dupays, N Towers, S Wood, A David, DJ Stuckey, T Mohun Furin, a transcriptional target of NKX2-5, has an essential role in heart development and function PLoS ONE, 2019;14(3):e0212992. 2019 [PMID: 30840660] (Western Blot, Mouse) Western Blot Mouse
AK Patel, K Vipparthi, V Thatikonda, I Arun, S Bhattachar, R Sharan, P Arun, S Singh A subtype of cancer-associated fibroblasts with lower expression of alpha-smooth muscle actin suppresses stemness through BMP4 in oral carcinoma Oncogenesis, 2018;7(10):78. 2018 [PMID: 30287850] (Neutralization, Human) Neutralization Human
N Blanchette, D Kita, A Konstorum, L Tesfay, D Lemler, P Hegde, KP Claffey, FM Torti, SV Torti Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer Oncogene, 2018;37(29):4013-4032. 2018 [PMID: 29695834] (Neutralization, Human) Neutralization Human
C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28747434] (Immunoprecipitation, Human) Immunoprecipitation Human
Herrera B, Inman GJ, A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. BMC Cell Biol., 2009;10(0):20. 2009 [PMID: 19298647] (Neutralization, Human) Neutralization Human
Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling. Blood, 2008;112(8):3154-63. 2008 [PMID: 18664625] (Neutralization, Human) Neutralization Human
Kane R, Godson C, O'Brien C Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis. Mol. Vis., 2008;14(0):1138-48. 2008 [PMID: 18587495] (Western Blot, Human) Western Blot Human
Zode GS, Clark AF, Wordinger RJ Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells. Invest. Ophthalmol. Vis. Sci., 2007;48(11):5058-67. 2007 [PMID: 17962458] (Western Blot, Human) Western Blot Human
Fuller ML, DeChant AK, Rothstein B, Caprariello A, Wang R, Hall AK, Miller RH Bone morphogenetic proteins promote gliosis in demyelinating spinal cord lesions. Ann. Neurol., 2007;62(3):288-300. 2007 [PMID: 17696121] (IHC-Fr, Rat) IHC-Fr Rat
Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach. J. Pathol., 2006;209(2):190-7. 2006 [PMID: 16550632] (IHC-P, Human) IHC-P Human
Show All 12 Publications.

Review for BMP-4 Antibody (MAB757) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB757:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot BMP-4 MAB757
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5
reviewed by:
Anonymous
WB Human 04/04/2020
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Summary

ApplicationWestern Blot
Sample Testedbreast cancer MCF7 cells,BT-20 human breast cancer cell line
SpeciesHuman
LotCEO011901B

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Recent Reviews

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5
1
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1
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Anonymous
04/04/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol BMP4
Uniprot