Recombinant Human Angiopoietin-1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Angiopoietin-1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J. et al. (1999) FEBS Letters 448:249. The ED50 for this effect is 10-40 ng/mL in the presence of 5 µg/mL of a
cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Mouse myeloma cell line, NS0-derived human Angiopoietin-1 protein
Ser20-Phe498, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser20
Structure / Form
Oligomer
Protein/Peptide Type
Recombinant Proteins
Gene
ANGPT1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70 kDa, reducing conditions
Publications
Read Publications using
923-AN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-1 Protein, CF

  • AGP1
  • AGPT
  • Ang1
  • ANG-1
  • angiopoietin 1
  • Angiopoietin-1
  • ANGPT1
  • KIAA0003

Background

Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system (1, 2). It contains a coiled-coil region and a C-terminal fibrinogen-like domain separated by a short flexible region (3, 4). Mature human Angiopoietin-1 shares 97% amino acid sequence identity with mouse and rat Angiopoietin-1. It is expressed by vascular smooth muscle cells and pericytes as an approximately 70 kDa molecule that associates into
disulfide-linked homotrimers, tetramers, and pentamers (3, 5). Angiopoietin-1 binds and activates the receptor tyrosine kinase Tie-2, and its association into tetramers is important for full Tie-2 activation (3, 4). Angiopoietin-1 ligation of Tie-2 on vascular endothelial cells (EC) induces the development and branching of blood vessels (6, 7). In sub-confluent EC (i.e. during angiogenesis), Angiopoietin-1 promotes EC motility and Tie-2 localization at the trailing edge of the cell (8). In confluent EC (i.e. in homeostasis), multimeric Angiopoietin-1 enhances vascular integrity by promoting the in trans homotypic association of Tie-2 between EC or with the substratum (8, 9). In addition, Angiopoietin-1 suppresses several VEGF-induced effects on the vasculature including endothelial permeability, stretch-induced release of Angiopoietin-2, and up-regulation of the leukocyte adhesion molecules VCAM-1, ICAM-1, and E-Selectin (10-12). Angiopoietin-1 also interacts with a variety of integrins and the extracellular matrix independently of Tie-2 (13, 14). These interactions support the adhesion, migration and stress resistance of EC, fibroblasts, and myocytes (13, 14). Angiopoietin-1 can protect against pulmonary arterial hypertension (5), reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium (15), and enhance tumor progression and metastasis (16).
  1. Koh, G.Y. (2012) Trends Mol. Med. 19:31.
  2. Suri, C. et al. (1996) Cell 87:1171.
  3. Davis, S. et al. (1996) Cell 87:1161.
  4. Kim, K.-T. et al. (2005) J. Biol. Chem. 280:20126.
  5. Kugathasan, L. et al. (2009) J. Exp. Med. 206:2221.
  6. Suri, C. et al. (1998) Science 282:468.
  7. Jeansson, M. et al. (2011) J. Clin. Invest. 121:2278.
  8. Saharinen, P. et al. (2008) Nat. Cell. Biol. 10:527.
  9. Fukuhara, S. et al. (2008) Nat. Cell Biol. 10:513.
  10. Jho, D. et al. (2005) Circ. Res. 96:1282.
  11. Korff, T. et al. (2012) Cardiovasc. Res. 94:510.
  12. Kim, I. et al. (2001) Circ. Res. 89:477.
  13. Carlson, T.R. et al. (2001) J. Biol. Chem. 276:26516.
  14. Dallabrida, S.M. et al. (2005) Cardiovasc. Res. 96:e8.
  15. Samuel, S.M. et al. (2010) Diabetes 59:51.
  16. Holopainen, T. et al. (2009) Cancer Res. 69:4656.

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Publications for Angiopoietin-1 (923-AN/CF)(64)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, N/A.

We have publications tested in 7 applications: Bioassay, Cell Culture, Control, ELISA (Capture), ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Bioassay
(50)
Cell Culture
(1)
Control
(1)
ELISA (Capture)
(1)
ELISA (Standard)
(3)
In Vivo
(6)
Western Blot
(1)
All Applications
Filter By Species
Human
(41)
Mouse
(14)
Rat
(2)
Bovine
(2)
N/A
(4)
All Species
Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using 923-AN/CF Applications Species
LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions PloS Pathogens, 2022;18(1):e1010280. 2022 [PMID: 35100311] (Bioassay, Human) Bioassay Human
T Shimazaki, N Noro, K Hagikura, T Matsumoto, C Yoshida-No Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy Biology, 2021;10(11):. 2021 [PMID: 34827205] (Bioassay, Human) Bioassay Human
R Luck, A Karakatsan, B Shah, G Schermann, H Adler, J Kupke, N Tisch, HW Jeong, MK Back, F Hetsch, A D'Errico, M De Palma, E Wiedtke, D Grimm, A Acker-Palm, J von Engelh, RH Adams, HG Augustin, C Ruiz de Al The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner Cell Reports, 2021;36(7):109522. 2021 [PMID: 34407407] (Bioassay, Mouse) Bioassay Mouse
Y Koya, H Nara, S Yagi, C Ueno, M Kamohara An engineered tetra-valent antibody fully activates the Tie2 receptor with comparable potency to its natural ligand angiopoietin-1 Scientific Reports, 2021;11(1):14021. 2021 [PMID: 34234265] (Bioassay, Human) Bioassay Human
S Fujiwara-S, Y Ueda, M Fujikawa, M Osaki, N Yamanaka, T Matsumoto Mucopolysaccharide polysulfate promotes microvascular stabilization and barrier integrity of dermal microvascular endothelial cells via activation of the angiopoietin-1/Tie2 pathway Journal of dermatological science, 2021;0(0):. 2021 [PMID: 34148739] (Bioassay, Human) Bioassay Human
I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk Nature Neuroscience, 2021;0(0):. 2021 [PMID: 33510480] (Bioassay, Mouse) Bioassay Mouse
S Harel, V Sanchez-Go, R Echavarria, D Mayaki, SN Hussain Roles of miR-640 and Zinc Finger Protein 91 (ZFP91) in Angiopoietin-1-Induced In Vitro Angiogenesis Cells, 2020;9(7):. 2020 [PMID: 32630670] (Cell Culture, Human) Cell Culture Human
S Gadomski, SK Singh, S Singh, T Sarkar, KD Klarmann, M Berenschot, S Seaman, B Jakubison, KO Gudmundsso, S Lockett, JR Keller Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration Cell Rep, 2020;31(4):107572. 2020 [PMID: 32348770] (Bioassay, Mouse) Bioassay Mouse
M Singhal, N Gengenbach, S La Porta, S Gehrs, J Shi, M Kamiyama, DM Bodenmille, A Fischl, B Schieb, E Besemfelde, S Chintharla, HG Augustin Preclinical validation of a novel metastasis-inhibiting Tie1 function-blocking antibody EMBO Mol Med, 2020;0(0):e11164. 2020 [PMID: 32302470] (Bioassay, Human) Bioassay Human
EA Thackaberr, Y Zhou, CL Zuch de Za, G Fuh, CV Lee, S Sanowar, JB Ridgway, AM Kusi, C Farman, H Booler, D Sheinson, CA Rasmussen, PE Miller, E Wakshull, M Yan, V Bantseev Rapid Development of Glaucoma Via ITV Nonselective ANGPT 1/2 Antibody: A Potential Role for ANGPT/TIE2 Signaling in Primate Aqueous Humor Outflow Invest. Ophthalmol. Vis. Sci., 2019;60(13):4097-4108. 2019 [PMID: 31574535] (Bioassay, Human) Bioassay Human
Show All 64 Publications.

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Bioinformatics

Gene Symbol ANGPT1
Uniprot