Recombinant Human 5T4 His-tag Avi-tag Protein, CF

Images

 
When Human 5T4 Antibody (Catalog # MAB49751) is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human 5T4 His-tag Avi-tag (Catalog # AVI10290) binds with an ED50 of 10-80 ng/mL.
2 μg/lane of Recombinant Human 5T4 His-tag Avi-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-80 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human 5T4 His-tag Avi-tag Protein, CF Summary

Additional Information
Biotinylated
Details of Functionality
Measured by its binding ability in a functional ELISA. When Human 5T4 Antibody (Catalog # MAB49751) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human 5T4 His-tag Avi-tag (Catalog # AVI10290) binds with an ED50 of 10-80 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human 5T4 protein
Human 5T4
(Ser31-Ser355)
Accession # Q13641.1
HHHHHH Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Ser31
Structure / Form
Biotinylated via Avi-tag
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-80 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human 5T4 His-tag Avi-tag Protein, CF

  • 5T4 oncofetal trophoblast glycoprotein
  • 5T4 oncotrophoblast glycoprotein
  • 5T4
  • 5T4AG
  • 5T4-AG
  • 5T4M6P1,5T4 oncofetal antigen
  • M6P1
  • TPBG
  • trophoblast glycoprotein

Background

5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling (1). Distinct regions on the 5T4 ectodomain which contains eight leucine-rich repeats are thought to antagonize Wnt signaling (2). 5T4 has limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival and prognosis (3-5). Human 5T4 is a 420 amino acid (aa) protein that includes a 31 aa signal peptide, a 324 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD, human 5T4 shares 78% and 77% aa sequence identity with mouse and rat 5T4, respectively. It has been identified that TIP-2/GIPC, a common interacting protein involved in cancer, interacts with 5T4 through its PDZ domain (6). 5T4 inhibits Wnt/ beta -catenin signaling by binding to LRP6 and inhibit Wnt-induced LRP6 internalization into endocytic vesicles (1). Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1 (1). In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled-8.
  1. Kagermeier-Schenk, B. et al. (2011) Dev. Cell. 21:1129.
  2. Zhao, Y. et al. (2014) Structure. 22:612.
  3. Starzynska, T. et al. (1994) Br. J. Cancer. 69:899.
  4. Naganuma, H. et al. (2002) Anticancer Res. 22:1033.
  5. Harris, J.L. et al. (2018) Int. J. Biochem. Cell Biol. 99:28.
  6. Awan, A. et al. (2002) BBRC 290:1030.

Customers Who Viewed This Item Also Viewed...

DLP00
Species: Hu
Applications: ELISA
MAB1790
Species: Hu
Applications: IHC, Simple Western, WB
NBP2-03886
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NBP1-83976
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-48309
Species: Hu, Mu, Rt
Applications: Flow-IC, Flow, ICC, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
AF972
Species: Hu
Applications: ELISA, IHC, KO, Simple Western, WB
NBP3-25538
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
AF748
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
MAB6896
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
AF1356
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
H00135138-D01P
Species: Hu, Mu
Applications: WB
AF1759
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
AF5070
Species: Hu
Applications: Simple Western, WB
268-N4/CF
Species: Hu
Applications: BA
NBP3-14348
Species: Hu
Applications: IHC, IHC-P
NBP2-48480
Species: Hu
Applications: Flow-CS, Flow, IHC, IHC-Fr, IHC-P
2845-GP
Species: Hu
Applications: Bind
NB500-517
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-85645
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AVI10290
Species: Hu
Applications: Bioactivity

Publications for 5T4 (AVI10290) (0)

There are no publications for 5T4 (AVI10290).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 5T4 (AVI10290) (0)

There are no reviews for 5T4 (AVI10290). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for 5T4 (AVI10290) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human 5T4 His-tag Avi-tag Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot