Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (Catalog # 10504-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 1.25-8 µg/mL.

2 μg/lane of Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (10504-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-54 kDa.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED₅₀ for this effect is 1.25-8 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey DC-SIGN/CD209 protein
  

  Hemagglutinin Tag (YPYDVPDYA)	Cynomolgus Monkey DC-SIGN/CD209 (Lys62-Glu406) Accession # XP_015296329.1
  N-terminus	C-terminus

• Accession #: XP_015296329.1
• N-terminal Sequence: Tyr of HA tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 44-54 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

• CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
• CD209 molecule
• CD209
• CDSIGNHIV gpl20-binding protein
• CLEC4L
• CLEC4LC-type lectin domain family 4 member L
• DCSIGN
• DC-SIGN
• DC-SIGN1
• DC-SIGN1C-type lectin domain family 4, member L
• DC-SIGNMGC129965
• Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 40 kDa, 344 amino acid (aa) type II transmembrane protein, with the extracellular domain (ECD) containing a Ca2+-dependent carbohydrate-binding lectin domain (2). Within the ECD, cynomolgus DC-SIGN/CD209 shares 85% sequence identity with human DC-SIGN/CD209. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self-glycoproteins including ICAMs (2, 3). DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (4,5).

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
3. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
4. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
5. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Bioinformatics

• Uniprot:
  • XP_015296329.1()