Recombinant Canine IL-17A Protein

• Reactivity: Ca
• Applications: Bioactivity

Recombinant Canine IL-17A Protein Summary

• Details of Functionality: Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED 50 for this effect is 0.3-1.5 ng/mL.
• Source: E. coli-derived canine IL-17/IL-17A protein Gly81-Ala210
• Accession #: XP_538958.2
• N-terminal Sequence: Gly81
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL17A
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 14 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Canine IL-17A Protein

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin 17 (IL-17; also IL-17A and CTLA-8) is a 17 kDa member of the IL-17 family of cytokines (1). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (2-4). Based on the amino acid (aa) sequence alignment with human IL-17, canine IL-17 is 130 aa in length. It is secreted as a 35 kDa disulfide-linked homodimer and as a 40 kDa disulfide-linked heterodimer with IL-17F (5). Canine IL-17 is 81% identical on the aa level to human IL-17. IL-23 drives Th17 lymphocytes to produce IL-17 (6-8). IL-17’s production has also been demonstrated in gamma δ T cells (9), CD8 + memory T cells (10, 11), eosinophils (12), neutrophils (10), and monocytes (13). Studies have identified that the widely expressed receptors IL-17RA and IL-17RC form a heterodimer for the binding of IL-17 (6, 14-15). The predominant function of IL-17 is thought to be as a proinflammatory mediator through a variety of mechanisms (16). Locally, IL-17 stimulates production of IL-6, prostaglandin E and nitric oxide (16-19), and synergy with other inflammatory cytokines such as TNF-alpha , IL-1 beta and IFN -gamma leads to up-regulation of gene expression and progression and amplification of local inflammation (16, 20-22). IL-17 also mediates chemotaxis of neutrophils and monocytes to sites of inflammation through the chemoattractant mediators IL-8, Gro-alpha , and MCP-1 (16, 22-25) while augmenting production of hematopoietic growth factors, such as G-CSF and GM-CSF (16, 26, 27), which promote the growth and maturation of the recruited myeloid cells. In addition, IL-17 serves as a bridge between innate and adaptive immune responses by enhancing the induction of co-stimulatory molecules such as ICAM-1 and other cytokines (16, 22, 28), thereby supporting T cell activation. IL-17 expression has been associated with many inflammatory diseases, such as rheumatoid arthritis, multiple sclerosis, asthma, systemic lupus erythematosus and allograft rejection (15).

1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
2. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
3. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
4. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
5. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
7. Steinman, L. (2007) Nat. Med. 13:139.
8. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
9. Lockhart, E. et al. (2006) J. Immunol. 177:4662.
10. Ferretti, S. et al. (2003) J. Immunol. 170:2106.
11. Shin, H.C. et al. (1999) Cytokine 11:257.
12. Molet, S. et al. (2001) J. Allergy Clin. Immunol. 108:430.
13. Zhou, Q. et al. (2005) Infect. Immun. 73:935.
14. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
15. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
16. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
17. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
18. Yao, Z. et al. (1995) Immunity 3:811.
19. Attur, M.G. et al. (1997) Arthritis Rheum. 40:1050.
20. Ruddy, M.J. et al. (2004) J. Biol. Chem. 279:2559.
21. Albanesi, C. et al. (1999) J. Immunol. 162:494.
22. Witowski, J. et al. (2000) J. Immunol. 165:5814.
23. Miyamoto, M. et al. (2003) J. Immunol. 170:4665.
24. Ye, P. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:335.
25. Laan, M. et al. (2001) Br. J. Pharmacol. 133:200.
26. Starnes, T. et al. (2002) J. Immunol. 169:642.
27. Jones, C.E. et al. (2002) Am. J. Respir. Cell Mol. Biol. 26:748.
28. Yao, Z. et al. (1995) J. Immunol. 155:5483.

Publications for IL-17/IL-17A (5848-CL)(1)

Publication using 5848-CL

• M Pelst, C Höbart, H de Rooster, B Devriendt, E Cox Immortalised canine buccal epithelial cells&#039; CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol Veterinary research, 2022-09-13;53(1):72. 2022-09-13 [PMID: 36100942] (Bioassay, Canine)

FAQs for IL-17/IL-17A (5848-CL). (Showing 1 - 2 of 2 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A
• Uniprot:
  • XP_538958.2()