RCOR1/CoREST Antibody

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Western Blot: RCOR1/CoREST Antibody [NB600-240] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CoREST antibody used for WB at 0.66 ...read more
Immunohistochemistry: RCOR1/CoREST Antibody [NB600-240] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-CoREST used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining ...read more
Western Blot: RCOR1/CoREST Antibody [NB600-240] - Detection of Human CoREST by Western Blot. Samples: Nuclear extract (5 and 25 mcg) from HeLa cells. Antibody: Affinity purified rabbit anti-CoREST antibody (NB600-240) ...read more
Immunohistochemistry-Paraffin: RCOR1/CoREST Antibody [NB600-240] - Human metastatic bone marrow. Affinity purified rabbit anti-CoREST used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry ...read more
Proximity Ligation Assay: RCOR1/CoREST Antibody [NB600-240] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HP1-beta (NB100-79774) and rabbit anti-human CoREST (NB600-240). Image merged from DAPI ...read more
Proximity Ligation Assay: RCOR1/CoREST Antibody [NB600-240] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human CoREST (NB600-240). Image merged from DAPI ...read more
Proximity Ligation Assay: RCOR1/CoREST Antibody [NB600-240] - Image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

RCOR1/CoREST Antibody Summary

Immunogen
A synthetic peptide that maps to a region between residues 425 and the C-terminus (residue 482) of human REST (RE1-silencing transcription factor) corepressor using the numbering given in entry NP_055971.1 (GeneID 23186)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RCOR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500-1:15000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
HeLa Lysate (NB800-PC9)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RCOR1/CoREST Antibody

  • CoREST
  • KIAA0071CORESTRCORREST corepressor
  • Protein CoREST
  • RCOR1
  • RCOR1/CoREST
  • REST corepressor 1

Background

Brain type II sodium channels are important in neuronal phenotyping and are only seen at high levels in neurons. Studies have shown that CoREST functions as a corepressor for REST. This interaction between REST and CoREST is accomplished by means of a single zinc finger binding motif originating from REST. Together, the REST-CoREST complex mediates long term repression of the type II sodium channel gene (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RCOR1
Entrez
Uniprot