ZNF217 Antibody (OTI3G5)

Western Blot: ZNF217 Antibody (3G5) [NBP2-46424] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ZNF217 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti ZNF217.(1:2000)

Immunohistochemistry-Paraffin: ZNF217 Antibody (3G5) [NBP2-46424] - Staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ZNF217 mouse monoclonal antibody. (Heat induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)

Immunohistochemistry-Paraffin: ZNF217 Antibody (3G5) [NBP2-46424] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti ZNF217 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry-Paraffin: ZNF217 Antibody (3G5) [NBP2-46424] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti ZNF217 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry-Paraffin: ZNF217 Antibody (3G5) [NBP2-46424] - Staining of paraffin-embedded Human lung tissue within the normal limits using anti ZNF217 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150)

Immunohistochemistry-Paraffin: ZNF217 Antibody (3G5) [NBP2-46424] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ZNF217 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI3G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ZNF217 Antibody (OTI3G5) Summary

• Description: Novus Biologicals Mouse ZNF217 Antibody (OTI3G5) (NBP2-46424) is a monoclonal antibody validated for use in IHC and WB. Anti-ZNF217 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 730-1048 of human ZNF217(NP_006517) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF217
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 115.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZNF217 Antibody (OTI3G5)

• DKFZp781O2147
• KIAA0335
• MGC125707
• MGC125710
• zinc finger protein 518
• zinc finger protein 518A
• ZNF518

Background

ZNF217 binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF217 Antibody (NBP2-46424)(1)

Publication using NBP2-46424

• Nate J. Fry, Brittany A. Law, Olga R. Ilkayeva, Kristen R. Carraway, Christopher L. Holley, Kyle D. Mansfield N 6 -methyladenosine contributes to cellular phenotype in a genetically-defined model of breast cancer progression Oncotarget 2018-07-27 [PMID: 30131850]

Bioinformatics

• Gene Symbol: ZNF217