RBP4/Retinol-Binding Protein 4 Antibody (008) - Azide and BSA Free

Flow Cytometry: RBP4/Retinol-Binding Protein 4 Antibody (008) [NBP2-90461] - Flow cytometric analysis of Rat CD59 expression on SD rat bone marrow cells. Cells were stained with purified anti-Rat CD59, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry/ Immunofluorescence: RBP4/Retinol-Binding Protein 4 Antibody (008) [NBP2-90461] - Immunofluorescence staining of RBP4/Retinol-Binding Protein 4 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90461 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).

• Reactivity: Mu
• Applications: Flow, ICC/IF
• Clone: 008
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RBP4/Retinol-Binding Protein 4 Antibody (008) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse RBP4/Retinol-Binding Protein 4 (Accession#: NP_035385.1; Met1-Leu201).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RBP4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for RBP4/Retinol-Binding Protein 4 Antibody (008) - Azide and BSA Free

• interstitial
• Plasma retinol-binding protein
• RBP4
• retinol binding protein 4, plasma
• RetinolBinding Protein 4
• retinol-binding protein 4, plasma

Background

RBP4 is a specific carrier for retinol in the blood. It is a member of the lipocalin protein family. Under physiological conditions, RBP binds transthyretin (TTR) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The RBP/TTR molar ration provides an indirect way to indicate marginal vitamin A deficiency. RBP4 may also play a role in insulin resistance in diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBP4