RanGAP1 Antibody

Images

 
Western Blot: RanGAP1 Antibody [NB100-1384] - (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: RanGAP1 Antibody [NB100-1384] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RanGAP1 Antibody Summary

Immunogen
Peptide with sequence ASEDIAKLAETLAK-C corresponding to N-Terminus according to NP_002874.1.
Localization
Cytoplasmic
Specificity
Please note that in mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.
Predicted Species
Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RANGAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.5 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human brain and 3T3 lysates (predicted MW of 68 kDa band according to NP_002874). IHC-P: Human liver shows vesicular staining in the cytoplasm of hepatocytes.
Control
NIH 3T3 Whole Cell Lysate
RanGAP1 Overexpression Lysate
Publications
Read Publication using NB100-1384.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RanGAP1 Antibody

  • Fug1
  • KIAA1835Fug1
  • MGC20266
  • Ran GTPase activating protein 1
  • ran GTPase-activating protein 1
  • RanGAP1
  • SD
  • segregation distorter homolog (Drosophila)
  • segregation distorter homolog
  • segregation distortion

Background

Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RanGAP1 Antibody (NB100-1384)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RANGAP1
Entrez
Uniprot