RanGAP1 Antibody

Images

 
Western Blot: RanGAP1 Antibody [NB100-1384] - (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: RanGAP1 Antibody [NB100-1384] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RanGAP1 Antibody Summary

Immunogen
Peptide with sequence ASEDIAKLAETLAK-C corresponding to N-Terminus according to NP_002874.1.
Localization
Cytoplasmic
Specificity
Please note that in mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.
Predicted Species
Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RANGAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.5 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human brain and 3T3 lysates (predicted MW of 68 kDa band according to NP_002874). IHC-P: Human liver shows vesicular staining in the cytoplasm of hepatocytes.
Control
NIH 3T3 Whole Cell Lysate
RanGAP1 Overexpression Lysate
Publications
Read Publication using NB100-1384.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RanGAP1 Antibody

  • Fug1
  • KIAA1835Fug1
  • MGC20266
  • Ran GTPase activating protein 1
  • ran GTPase-activating protein 1
  • RanGAP1
  • SD
  • segregation distorter homolog (Drosophila)
  • segregation distorter homolog
  • segregation distortion

Background

Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
210-TA
Species: Hu
Applications: BA
H00005555-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
AF5415
Species: Hu
Applications: ICC, IHC, Simple Western, WB
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
291-G1
Species: Hu
Applications: BA
D6050
Species: Hu
Applications: ELISA
H00003845-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IP, WB
AF1638
Species: Hu, Mu, Rt
Applications: ICC, Simple Western, WB
AF1067
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
AF6989
Species: Mu
Applications: IHC
AF1067
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
H00342184-M07
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP1-90927
Species: Hu
Applications: IHC, IHC-P, WB
NB300-164
Species: Ca, Hu, Mu, Po, Pm, Rt(-)
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
DCRP00
Species: Hu
Applications: ELISA
NBP1-89953
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
DVE00
Species: Hu
Applications: ELISA
NBP1-30027
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for RanGAP1 Antibody (NB100-1384)(1)

Reviews for RanGAP1 Antibody (NB100-1384) (0)

There are no reviews for RanGAP1 Antibody (NB100-1384). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RanGAP1 Antibody (NB100-1384) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional RanGAP1 Products

Bioinformatics Tool for RanGAP1 Antibody (NB100-1384)

Discover related pathways, diseases and genes to RanGAP1 Antibody (NB100-1384). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RanGAP1 Antibody (NB100-1384)

Discover more about diseases related to RanGAP1 Antibody (NB100-1384).
 

Pathways for RanGAP1 Antibody (NB100-1384)

View related products by pathway.

PTMs for RanGAP1 Antibody (NB100-1384)

Learn more about PTMs related to RanGAP1 Antibody (NB100-1384).
 

Research Areas for RanGAP1 Antibody (NB100-1384)

Find related products by research area.

Blogs on RanGAP1

There are no specific blogs for RanGAP1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RanGAP1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol RANGAP1
Entrez
Uniprot