RanBP3 Antibody

Western Blot: RanBP3 Antibody [NB120-2939] - Analysis of RanBP3 from HeLa cytosol.

Immunohistochemistry: RanBP3 Antibody [NB120-2939] - Figure 1: IHC staining of RanBP3 in NRK cells. Figure 2: WB of RanBP3 from HeLa cytosol.

Product Discontinued

RanBP3 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues M(1) A D L A N E E K P A L A(13) C of human RanBP3.
• Specificity: Detects Ran binding protein 3 (RanBP 3) from human and rat cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RANBP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 10 ug/ml
• Application Notes: WB: Detects an approx. 60 kDa protein representing RanBP 3 from HeLa cell extracts. IF: Staining of RanBP 3 in NRK cells results in diffuse nuclear staining.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RanBP3 Antibody

• DKFZp586I1520
• RAN binding protein 3
• ran-binding protein 3
• RAN-binding protein-3
• RanBP3

Background

Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC, associated transport proteins and GTP. The GTPase Ran is a small protein that belongs to the RAS protein superfamily and is found associated with the nuclear membrane. Ran GTPase is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. Ran binding proteins (RanBPs) belong to a family of proteins that bind Ran GTPase and help to stimulate its GTPase activity. Members of the RanBP family show weak similarity to importin beta, a protein involved in the transport of proteins to the nuclear membrane. RanBP3 functions as a cofactor for CRM1-mediated nuclear export and stimulates export by enhancing the affinity of CRM1 for RanGTP and substrate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RANBP3
• Entrez:
  • 8498(Human)
  • 501281(Rat)